Detection of peptide-based nanoparticles in blood plasma by ELISA

Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions

Individual, family and offence characteristics of high risk childhood offenders: comparing non-offending, one-time offending and re-offending Dutch-Moroccan migrant children in the Netherlands

<p>Abstract</p> <p>Background</p> <p>Childhood offenders are at an increased risk for developing mental health, social and educational problems later in life. An early onset of offending is a strong predictor for future persistent offending. Childhood offenders from ethnic minority groups are a vulnerable at-risk group. However, up until now, no studies have focused on them.</p> <p>Aims</p> <p>To investigate which risk factors are associated with (re-)offending of childhood offenders from an ethnic minority.</p> <p>Method</p> <p>Dutch-Moroccan boys, who were registered by the police in the year 2006-2007, and their parents as well as a control group (n = 40) were interviewed regarding their individual and family characteristics. Two years later a follow-up analysis of police data was conducted to identify one-time offenders (n = 65) and re-offenders (n = 35).</p> <p>Results</p> <p>All groups, including the controls, showed substantial problems. Single parenthood (OR 6.0) and financial problems (OR 3.9) distinguished one-time offenders from controls. Reading problems (OR 3.8), having an older brother (OR 5.5) and a parent having Dutch friends (OR 4.3) distinguished re-offenders from one-time offenders. First offence characteristics were not predictive for re-offending. The control group reported high levels of emotional problems (33.3%). Parents reported not needing help for their children but half of the re-offender's families were known to the Child Welfare Agency, mostly in a juridical framework.</p> <p>Conclusion</p> <p>The Moroccan subgroup of childhood offenders has substantial problems that might hamper healthy development. Interventions should focus on reaching these families tailored to their needs and expectations using a multi-system approach.</p

Advances in InGaAsP-based avalanche diode single photon detectors

In this Topical Review, we survey the state-of-the-art of single photon detectors based on avalanche diodes fabricated in the InGaAsP materials system for photon counting at near infrared wavelengths in the range from 0.9–1.6 mm. The fundamental trade-off between photon detection efficiency and dark count rate can now be managed with performance that adequately serves many applications, with low dark count rates of ~ 1 kHz having been demonstrated at photon detection efficiencies of 20% for 25 mm diameter fiber-coupled devices with thermoelectric cooling. Timing jitter of less than 50 ps has been achieved, although device uniformity is shown to be essential in obtaining good jitter performance. Progress is also reported towards resolving the limitations imposed on photon counting rate by afterpulsing, with at least 50MHz repetition frequencies demonstrated for 1 ns gated operation with afterpulsing limited to the range of 1–5%. We also present a discussion of future trends and challenges related to these devices organized according to the hierarchy of materials properties, device design concepts, signal processing and electronic circuitry, and multiplexing concepts. Whereas the materials properties of these devices may pose significant challenges for the foreseeable future, there has been considerable progress in device concepts and circuit solutions towards the present imperatives for higher counting rates and simpler device operation

Biodegradable nano- and microparticles with controlled surface properties

Methods suitable for formation of polyester nano- and microspheres with controlled surface properties are described. The title nano-and microspheres composed of polylactides or poly(F-caprolactone) were obtained either directly, during dispersion polymerization of corresponding monomers, or by self-assembly of block copolymers containing polyester blocks. Surface properties of the particles were controlled by using surfactants with required functional groups for dispersion polymerization, by modification of interfacial layer of synthesized particles, and by using for self-assembly copolymers with appropriate functional groups. Particles with alkyl, amino, hydroxyl, carboxyl, and 4-(phenyl-azo)phenyl groups in interfacial layer were obtained. Stability of suspensions of particles and particle's integrity did depend on the nature of functional groups in their interfacial layer and on external stimuli such as pH of suspending medium and irradiation with UV ligh

Single photon avalanche diodes (SPADs) for 1.5 µm photon counting applications

The paper reports on the design and characterization of InGaAs/InP single photon avalanche diodes (SPADs) for photon counting applications at wavelengths near 1.5 mm. It is shown how lower internal electric field amplitudes can lead to reduced dark count rates, but at the expense of degraded afterpulsing behaviour and larger timing jitter. Dark count rate behaviour provides evidence of thermally assisted tunnelling with an average thermal activation energy of ~0.14 eV between 150K and 220 K. Afterpulsing behaviour exhibits a structure-dependent afterpulsing activation energy, which quantifies how carrier de-trapping varies with temperature. SPAD performance simultaneously exhibits a dark count rate of 10 kHz at a detection efficiency of 20% with timing jitter of 100 ps at 200 K, and with appropriate performance tradeoffs, we demonstrate a 200K dark count rate as low as 3 kHz, a detection efficiency as high as 45%, and a timing jitter as low as 30 ps