Theory and calibration of speckle dynamics of phase object

The optical system consisting of a laser, a thin three-dimensional diffuser, lens and image plane of the diffuser was considered. Theoretically the dynamics of speckle, which arises when you make a dynamic phase object between the diffuser and the lens was investigated. An expressions for the time correlation function and time spectral function of intensity of the scattered radiation was obtained. It is shown that output of the correlation function on the plateau, the peak position and width of the spectral function are the values by which one can control the parameters of the dynamic phase object. In the experiment, the dynamics of speckles by moving the glass plate having surface roughness Ra = 0,01 μm was created. The correctness of the formulas for the time autocorrelation function was tested. It is shown that variations in the optical path lengths found from the plateau value and roughness differ by 5%.?he possibility of applying the theoretical results for the control of biological and technical objects are discussed. © 2012 SPIE

A speckle-interferometric device for studying the cell biological activity

A modernized device intended for diagnosing the temperature-dependent activity of cells is described. The activity of cells was determined by finding the correlation coefficient of optical signals in speckle patterns recorded at different moments of time. It is shown that, as the temperature increases from 26 to 36Y°C, variations in the optical paths in cells increase from 16 to 26 nm. This is probably associated with the physiological activity of cells-trapping of large molecules or their conglomerates. In contrast to the pre- vious variant of the device design, instead of a lipid membrane, a cell monolayer was used, which was culti- vated on a plane-parallel glass plate. In order to reduce variations in the optical path lengths in a nutrient solution, a 1.7-mm-thick plane-parallel glass plate was additionally inserted into the cuvette. The modern- ization of the device also includes heating of the cuvette with hot air flows. © Pleiades Publishing, Ltd., 2012