DNA-Mediated Electron Transfer in Naphthalene-Modified Oligonucleotides

Naphthalene-modified oligonucleotides have been synthesized and characterized with respect to electron transfer chemistry. Using the Sonogashira coupling reaction, naphthalene can be covalently anchored onto a modified uridine through an ethynyl linkage. This tethering allows for effective electronic coupling with the DNA bases, resulting in a significant red shift of the absorption bands of the naphthalenic chromophore. Modification with this chromophore does not appear to affect the overall stability and structure of the DNA. Upon selective irradiation of the naphthalene moiety at 340 nm, photoreduction of a distal electron trap, 5-bromouridine, embedded in the DNA base stack occurs. This DNA-mediated reduction from a distance was found to be significantly more efficient with substitution of 5-bromouridine toward the 5′-end than toward the 3′-end. These results support a general preference for electron transfer through DNA toward the 5′-end, irrespective of the donor. In addition, differences in efficiency of photoreduction through intrastrand and interstrand pathways are observed. For DNA-mediated reduction, as with DNA-mediated oxidation, significant differences in the charge transfer reaction are apparent that depend upon subtle differences in coupling into the DNA base stack

The African Lungfish (\u3cem\u3eProtopterus dolloi\u3c/em\u3e): Ionoregulation and Osmoregulation in a Fish out of Water

Although urea production and metabolism in lungish have been thoroughly studied, we have little knowledge of how internal osmotic and electrolyte balance are controlled during estivation or in water. We tested the hypothesis that, compared with the body surface of teleosts, the slender African lungfish (Protopterus dolloi) body surface was relatively impermeable to water, Na+ and Cl- due to its greatly reduced gills. Accordingly, we measured the tritiated water (3H-H2O) flux in P. dolloi in water and during air exposure. In water, 3H-H2O efflux was comparable with the lowest measurements reported in freshwater teleosts, with a rate constant (K) of 17.6% body water h-1. Unidirectional ion fluxes, measured using 22Na+ and 36Cl-, indicated that Na+ and Cl- influx was more than 90% lower than values reported in most freshwater teleosts. During air exposure, a cocoon formed within 1 wk that completely covered the dorsolateral body surface. However, there were no disturbances to blood osmotic or ion (Na+, Cl-) balance, despite seven- to eightfold increases in plasma urea after 20 wk. Up to 13-fold increases in muscle urea (on a dry-weight basis) were the likely explanation for the 56% increase in muscle water content observed after 20 wk of air exposure. The possibility that muscle acted as a “water reservoir” during air exposure was supported by the 20% decline in body mass observed during subsequent reimmersion in water. This decline in body mass was equivalent to 28 mL water in a 100-g animal and was very close to the calculated net water gain (approximately 32 mL) observed during the 20-wk period of air exposure. Tritiated water and unidirectional ion fluxes on air-exposed lungfish revealed that the majority of water and ion exchange was via the ventral body surface at rates that were initially similar to aquatic rates. The 3H-H2O flux declined over time but increased upon reimmersion. We conclude that the slender lungfish body surface, including the gills, has relatively low permeability to water and ions but that the ventral surface is an important site of osmoregulation and ionoregulation. We further propose that an amphibian-like combination of ventral skin water and ion permeability, plus internal urea accumulation during air exposure, allows P. dolloi to extract water from its surroundings and to store water in the muscle when the water supply becomes limited

Association Between the COVID-19 Pandemic and Early Childhood Development

コロナ禍で5歳児に約4か月の発達の遅れ --3歳、5歳ともに発達の個人差拡大--. 京都大学プレスリリース. 2023-07-11.[Importance] Although a growing number of studies have reported negative associations of the COVID-19 pandemic with academic performance among school-aged children, less is known about the pandemic’s association with early childhood development. [Objective] To examine the association between the COVID-19 pandemic and early childhood development. [Design, Setting, and Participants] In this cohort study conducted in all accredited nursery centers in a Japanese municipality, baseline surveys of children aged 1 and 3 years (1000 and 922, respectively) were conducted between 2017 and 2019, and participants were followed up for 2 years. [Exposure] Children’s development was compared at age 3 or 5 years between cohorts that were exposed to the pandemic during the follow-up and a cohort that was not. [Main Outcome and Measure] Children’s developmental age was measured by nursery teachers using the Kinder Infant Development Scale (KIDS). Data were analyzed between December 8, 2022, and May 6, 2023. [Results] A total of 447 children (201 girls [45.0%] and 246 boys [55.0%]) aged 1 year at baseline were followed up to age 3 years, and 440 children (200 girls [45.5%] and 240 boys [54.5%]) aged 3 years at baseline were followed up to age 5 years. During the follow-up, the cohorts that were exposed to the pandemic were 4.39 months behind in development at age 5 compared with the cohort that was not (coefficient, −4.39; 95% credible interval, −7.66 to −1.27). Such a negative association was not observed in development at age 3 years (coefficient, 1.32; 95% credible interval, −0.44 to 3.01). Variations in development were greater during the pandemic than before the pandemic regardless of age. Additionally, the quality of care at nursery centers was positively associated with development at age 3 years during the pandemic (coefficient, 2.01; 95% credible interval, 0.58-3.44), while parental depression appeared to amplify the association between the pandemic and delayed development at age 5 (coefficient of interaction, −2.62; 95% credible interval, −4.80 to −0.49; P = .009). [Conclusions and Relevance] The findings of this study showed an association between exposure to the pandemic and delayed childhood development at age 5 years. Variations in development widened during the pandemic regardless of age. It is important to identify children with developmental delays associated with the pandemic and provide them with support for learning, socialization, physical and mental health, and family support

Geochemistry and Sm‐Nd chronology of a Stannern‐group eucrite, Northwest Africa 7188

We report the results of a detailed study of the basaltic eucrite Northwest Africa (NWA) 7188, including its mineralogical and bulk geochemical characteristics, oxygen isotopic composition, and 147,146Sm‐143,142Nd mineral isochron ages. The texture and chemical composition of pyroxene and plagioclase demonstrate that NWA 7188 is a monomict eucrite with a metamorphic grade of type 4. The oxygen isotopic composition and the Fe/Mn ratios of pyroxene confirmed that NWA 7188 belongs to the howardite–eucrite–diogenite meteorite suite, generally considered to originate from asteroid 4 Vesta. Whole‐rock TiO2, La, and Hf concentrations and a CI chondrite‐normalized rare earth element pattern are in good agreement with those of representative Stannern‐group eucrites. The 147,146Sm‐143,142Nd isochrons for NWA 7188 yielded ages of 4582 ± 190 and 4554 +17/−19 Ma, respectively. The closure temperature of the Sm‐Nd system for different fractions of NWA 7188 was estimated to be >865 °C, suggesting that the Sm‐Nd decay system has either been resistant to reheating at ~800 °C during the global metamorphism or only partially reset. Therefore, the 146Sm‐142Nd age of NWA 7188 corresponds to the period of initial crystallization of basaltic magmas and/or global metamorphism on the parent body, and is unlikely to reflect Sm‐Nd disturbance by late reheating and impact events. In either case, NWA 7188 is a rare Stannern‐group eucrite that preserves the chronological information regarding the initial crustal evolution of Vesta

Noble Gases in the Chelyabinsk Meteorites

The Chelyabinsk meteorite fell in Russia on February 15, 2013 and was classified as LL5 chondrite. The diameter before it entered the atmosphere has been estimated to be about 20 m [1]. Up to now, numerous fragments weighing much greater than 100 kg in total have been collected. In this study, all noble gases were measured for 13 fragments to investigate the exposure history of the Chelyabinsk meteorite and the thermal history of its parent asteroid

The Subaru/XMM-Newton Deep Survey (SXDS). IV. Evolution of Lya Emitters from z=3.1 to 5.7 in the 1 deg^2 Field: Luminosity Functions and AGN

We present luminosity functions (LFs) and various properties of Lya emitters (LAEs) at z=3.1, 3.7, and 5.7, in a 1 deg^2 sky of the Subaru/XMM-Newton Deep Survey (SXDS) Field. We obtain a photometric sample of 858 LAE candidates based on deep Subaru/Suprime-Cam imaging data, and a spectroscopic sample of 84 confirmed LAEs from Subaru/FOCAS and VLT/VIMOS spectroscopy in a survey volume of ~10^6 Mpc^3 with a limiting Lya luminosity of ~3x10^42 erg/s. We derive the LFs of Lya and UV-continuum (~1500 \AA) for each redshift, taking into account the statistical error and the field-to-field variation. We find that the apparent Lya LF shows no significant evolution between z=3.1 and 5.7 within factors of 1.8 and 2.7 in L* and phi*, respectively. On the other hand, the UV LF of LAEs increases from z=3.1 to 5.7, indicating that galaxies with Lya emission are more common at earlier epochs. We identify six LAEs with AGN activities from our spectra combined with VLA, Spitzer, and XMM-Newton data. Among the photometrically selected LAEs at z=3.1 and 3.7, only ~1 % show AGN activities, while the brightest LAEs with logL(Lya) >~ 43.4-43.6 erg/s appear to always host AGNs. Our LAEs are bluer in UV-continuum color than dropout galaxies, suggesting lower extinction and/or younger stellar populations. Our stacking analyses provide upper limits to the radio luminosity and the f(HeII)/f(Lya) line fraction, and constrain the hidden star formation (+low-luminosity AGN) and the primordial population in LAEs.Comment: 75 pages, 27 figures; ApJS in press. High resolution version at http://www.ociw.edu/~ouchi/work/astroph/sxds_LAEs/ouchi_SXDSLAE_ApJS.pd