42 research outputs found
Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum
<p>Abstract</p> <p>Background</p> <p>Two sets of overlapping genes, <it>lacLMReu </it>and <it>lacLMAci</it>, encoding heterodimeric β-galactosidases from <it>Lactobacillus reuteri </it>and <it>Lactobacillus acidophilus</it>, respectively, have previously been cloned and expressed using the pSIP vector system and <it>Lactobacillus plantarum </it>WCSF1 as host. Despite the high similarity between these <it>lacLM </it>genes and the use of identical cloning and expression strategies, strains harboring <it>lacLMReu </it>produced about twenty-fold more β-galactosidase than strains containing <it>lacLMAci</it>.</p> <p>Results</p> <p>In this study, the plasmid copy numbers (PCN) of expression vectors pEH9R (<it>lacLMReu</it>) and pEH9A (<it>lacLMAci</it>) as well as the transcription levels of both <it>lacLM </it>genes were compared using quantitative PCR methods. Analyses of parallel fermentations of <it>L. plantarum </it>harboring either pEH9R or pEH9A showed that the expression plasmids were present in similar copy numbers. However, transcript levels of <it>lacLM </it>from <it>L. reuteri </it>(pEH9R) were up to 18 times higher than those of <it>lacLM </it>from <it>L. acidophilus </it>(pEH9A). As a control, it was shown that the expression levels of regulatory genes involved in pheromone-induced promoter activation were similar in both strains.</p> <p>Conclusion</p> <p>The use of identical expression strategies for highly similar genes led to very different mRNA levels. The data indicate that this difference is primarily caused by translational effects that are likely to affect both mRNA synthesis rates and mRNA stability. These translational effects thus seem to be a dominant determinant for the success of gene expression efforts in lactobacilli.</p
Triple Michelson Interferometer for a Third-Generation Gravitational Wave Detector
The upcoming European design study `Einstein gravitational-wave Telescope'
represents the first step towards a substantial, international effort for the
design of a third-generation interferometric gravitational wave detector. It is
generally believed that third-generation instruments might not be installed
into existing infrastructures but will provoke a new search for optimal
detector sites. Consequently, the detector design could be subject to fewer
constraints than the on-going design of the second generation instruments. In
particular, it will be prudent to investigate alternatives to the traditional
L-shaped Michelson interferometer. In this article, we review an old proposal
to use three Michelson interferometers in a triangular configuration. We use
this example of a triple Michelson interferometer to clarify the terminology
and will put this idea into the context of more recent research on
interferometer technologies. Furthermore the benefits of a triangular detector
will be used to motivate this design as a good starting point for a more
detailed research effort towards a third-generation gravitational wave
detector.Comment: Minor corrections to the main text and two additional appendices. 14
pages, 6 figure
Removing non-stationary, non-harmonic external interference from gravitational wave interferometer data
We describe a procedure to identify and remove a class of non-stationary and
non-harmonic interference lines from gravitational wave interferometer data.
These lines appear to be associated with the external electricity main
supply, but their amplitudes are non-stationary and they do not appear at
harmonics of the fundamental supply frequency. We find an empirical model able
to represent coherently all the non-harmonic lines we have found in the power
spectrum, in terms of an assumed reference signal of the primary supply input
signal. If this signal is not available then it can be reconstructed from the
same data by making use of the coherent line removal algorithm that we have
described elsewhere. All these lines are broadened by frequency changes of the
supply signal, and they corrupt significant frequency ranges of the power
spectrum. The physical process that generates this interference is so far
unknown, but it is highly non-linear and non-stationary. Using our model, we
cancel the interference in the time domain by an adaptive procedure that should
work regardless of the source of the primary interference. We have applied the
method to laser interferometer data from the Glasgow prototype detector, where
all the features we describe in this paper were observed. The algorithm has
been tuned in such a way that the entire series of wide lines corresponding to
the electrical interference are removed, leaving the spectrum clean enough to
detect signals previously masked by them. Single-line signals buried in the
interference can be recovered with at least 75 % of their original signal
amplitude.Comment: 14 pages, 5 figures, Revtex, psfi
Gravitational-wave research as an emerging field in the Max Planck Society. The long roots of GEO600 and of the Albert Einstein Institute
On the occasion of the 50th anniversary since the beginning of the search for
gravitational waves at the Max Planck Society, and in coincidence with the 25th
anniversary of the foundation of the Albert Einstein Institute, we explore the
interplay between the renaissance of general relativity and the advent of
relativistic astrophysics following the German early involvement in
gravitational-wave research, to the point when gravitational-wave detection
became established by the appearance of full-scale detectors and international
collaborations. On the background of the spectacular astrophysical discoveries
of the 1960s and the growing role of relativistic astrophysics, Ludwig Biermann
and his collaborators at the Max Planck Institute for Astrophysics in Munich
became deeply involved in research related to such new horizons. At the end of
the 1960s, Joseph Weber's announcements claiming detection of gravitational
waves sparked the decisive entry of this group into the field, in parallel with
the appointment of the renowned relativist Juergen Ehlers. The Munich area
group of Max Planck institutes provided the fertile ground for acquiring a
leading position in the 1970s, facilitating the experimental transition from
resonant bars towards laser interferometry and its innovation at increasingly
large scales, eventually moving to a dedicated site in Hannover in the early
1990s. The Hannover group emphasized perfecting experimental systems at pilot
scales, and never developed a full-sized detector, rather joining the LIGO
Scientific Collaboration at the end of the century. In parallel, the Max Planck
Institute for Gravitational Physics (Albert Einstein Institute) had been
founded in Potsdam, and both sites, in Hannover and Potsdam, became a unified
entity in the early 2000s and were central contributors to the first detection
of gravitational waves in 2015.Comment: 94 pages. Enlarged version including new results from further
archival research. A previous version appears as a chapter in the volume The
Renaissance of General Relativity in Context, edited by A. Blum, R. Lalli and
J. Renn (Boston: Birkhauser, 2020