Computational analysis to predict functional role of hsa-miR-3065-3p as an antiviral therapeutic agent for treatment of triple infections: HCV, HIV-1, and HBV

Background: Triple infection (TI) with HIV-1, HCV, and HBV (TI) is highly prevalent in intravenous drug users (IDUs). These TI patients have a faster progression to AIDS, and even after antiretroviral therapy (ART) the prognosis of their disease is poor. The use of microRNA (miRNA) to silence genes holds potential applications for anti-HCV therapy. Methods: We analyzed the role of human miRNAs (hsa-miRs) in TI by computational analyses for HCV, HIV-1, and HBV showing identity to these three viral genomes. Results: We identified one unique miRNA, hsa-miR-3065-3p, that shares significant mutual identity to these three viral genomes (6183%). In addition, hsa-miR-99, hsa-miR-548, and hsa-miR-122 also showed mutual identity with these three viral genomes, albeit at a lower degree (5288%). Conclusion: Here, we present evidence using essential components of bioinformatics tools, and hypothesize that utility of hsa-miR-3065-3p and perhaps miR-548 would be potential antiviral therapeutic agents in the treatment of TI patients because it shows near perfect alignment in the seed region for all three viruses. We also make an argument that current proposed therapy with hsa-miR-122 may not be the optimal choice for HCV patients since it lacks essential gene alignment and may be harmful for the patients.Keywords: HBV; HCV; HIV-1; homology; identity; inhibition; microRNA-based therapy; RNAi; triple infectio

Cultural diversity in the curriculum: perceptions and attitudes of Irish hospitality and tourism academics

Academics are facing significant challenges in preparing indigenous students for employment in the multicultural working environment of hospitality and tourism organisations. In dealing with the impact of the new skills and flexibilities demanded by increasing globalisation, the indigenous workforce needs to possess a multicultural perspective and the adaptive skills and mindsets to integrate more inclusively in diverse work settings. This article explores the nature of cultural diversity within the Irish hospitality and tourism workforce (in both Northern Ireland and the Republic of Ireland). An attempt is made to identify the challenges that hospitality and tourism educators face in implementing education for cultural diversity within the curriculum in order to prepare indigenous students (and indeed local industry staff) for employment within a multicultural workforce. The main themes analysed include: training requirements; educators' requirements; programme content and learning outcomes; assessment methods; and methods of programme delivery