Structure-based functional inference of hypothetical proteins from Mycoplasma hyopneumoniae

Enzootic pneumonia caused by Mycoplasma hyopneumoniae is a major constraint to efficient pork production throughout the world. This pathogen has a small genome with 716 coding sequences, of which 418 are homologous to proteins with known functions. However, almost 42% of the 716 coding sequences are annotated as hypothetical proteins. Alternative methodologies such as threading and comparative modeling can be used to predict structures and functions of such hypothetical proteins. Often, these alternative methods can answer questions about the properties of a model system faster than experiments. In this study, we predicted the structures of seven proteins annotated as hypothetical in M. hyopneumoniae, using the structure-based approaches mentioned above. Three proteins were predicted to be involved in metabolic processes, two proteins in transcription and two proteins where no function could be assigned. However, the modeled structures of the last two proteins suggested experimental designs to identify their functions. Our findings are important in diminishing the gap between the lack of annotation of important metabolic pathways and the great number of hypothetical proteins in the M. hyopneumoniae genome

Burnout in Organizational Life

Burnout is a psychological response to work stress that is characterized by emotional exhaustion, depersonalization, and reduced feelings of personal accomplishment. In this paper, we review the burnout literature from 1993 to present, identifying important trends that have characterized the literature. We focus our attention on theoretical models that explain the process of burnout, the measurement of burnout, means of reducing burnout, and directions for the future of burnout research.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

The Effects of Commitment to Multiple Foci: An Analysis of Relative Influence and Interactions

Recent research on employee commitment has adopted a multifoci framework, arguing that it is important to distinguish between commitments to particular targets such as supervisors, coworkers, and the organization itself. However, there remain questions about the relative importance of different commitments for predicting outcomes and about possible interactive effects among multiple commitments. To address these questions, the authors collected data from 279 employees as well as their supervisors and coworkers. The authors found robust effects for affective commitment to supervisors, showing that it was significantly related to turnover intentions, task performance, and citizenship behaviors incremental to affective commitment to the organization and coworkers. In addition, a 3-way commitment interaction predicted task performance and citizenship behaviors directed toward the supervisor