500 research outputs found
A Review and Outlook for the Removal of Radon-Generated Po-210 Surface Contamination
The next generation low-background detectors operating deep underground aim
for unprecedented low levels of radioactive backgrounds. The deposition and
presence of radon progeny on detector surfaces is an added source of energetic
background events. In addition to limiting the detector material's radon
exposure in order to reduce potential surface backgrounds, it is just as
important to clean surfaces to remove inevitable contamination. Such studies of
radon progeny removal have generally found that a form of etching is effective
at removing some of the progeny (Bi and Pb), however more aggressive
techniques, including electropolishing, have been shown to effectively remove
the Po atoms. In the absence of an aggressive etch, a significant fraction of
the Po atoms are believed to either remain behind within the surface or
redeposit from the etching solution back onto the surface. We explore the
chemical nature of the aqueous Po ions and the effect of the oxidation state of
Po to maximize the Po ions remaining in the etching solution of contaminated Cu
surfaces. We present a review of the previous studies of surface radon progeny
removal and our findings on the role of oxidizing agents and a cell potential
in the preparation of a clean etching technique.Comment: Proceedings of the Low Radioactivity Techniques (LRT) 2017, Seoul,
South Korea, May 24-26, 201
A genome-wide RNAi screen reveals multiple regulators of caspase activation
Apoptosis is an evolutionally conserved cellular suicide mechanism that can be activated in response to a variety of stressful stimuli. Increasing evidence suggests that apoptotic regulation relies on specialized cell death signaling pathways and also integrates diverse signals from additional regulatory circuits, including those of cellular homeostasis. We present a genome-wide RNA interference screen to systematically identify regulators of apoptosis induced by DNA damage in Drosophila melanogaster cells. We identify 47 double- stranded RNA that target a functionally diverse set of genes, including several with a known function in promoting cell death. Further characterization uncovers 10 genes that influence caspase activation upon the removal of Drosophila inhibitor of apoptosis 1. This set includes the Drosophila initiator caspase Dronc and, surprisingly, several metabolic regulators, a candidate tumor suppressor, Charlatan, and an N-acetyltransferase, ARD1. Importantly, several of these genes show functional conservation in regulating apoptosis in mammalian cells. Our data suggest a previously unappreciated fundamental connection between various cellular processes and caspase-dependent cell death
Development of a transmission model for dengue virus
BACKGROUND: Dengue virus (DENV) research has historically been hampered by the lack of a susceptible vertebrate transmission model. Recently, there has been progress towards such models using several varieties of knockout mice, particularly those deficient in type I and II interferon receptors. Based on the critical nature of the type I interferon response in limiting DENV infection establishment, we assessed the permissiveness of a mouse strain with a blunted type I interferon response via gene deficiencies in interferon regulatory factors 3 and 7 (IRF3/7 (−/− −/−)) with regards to DENV transmission success. We investigated the possibility of transmission to the mouse by needle and infectious mosquito, and subsequent transmission back to mosquito from an infected animal during its viremic period. METHODS: Mice were inoculated subcutaneously with non-mouse adapted DENV-2 strain 1232 and serum was tested for viral load and cytokine production each day. Additionally, mosquitoes were orally challenged with the same DENV-2 strain via artificial membrane feeder, and then allowed to forage or naïve mice. Subsequently, we determined acquisition potential by allowing naïve mosquitoes on forage on exposed mice during their viremic period. RESULTS: Both needle inoculation and infectious mosquito bite(s) resulted in 100% infection. Significant differences between these groups in viremia on the two days leading to peak viremia were observed, though no significant difference in cytokine production was seen. Through our determination of transmission and acquisition potentials, the transmission cycle (mouse-to mosquito-to mouse) was completed. We confirmed that the IRF3/7 (−/− −/−) mouse supports DENV replication and is competent for transmission experiments, with the ability to use a non-mouse adapted DENV-2 strain. A significant finding of this study was that this IRF3/7 (−/− −/−) mouse strain was able to be infected by and transmit virus to mosquitoes, thus providing means to replicate the natural transmission cycle of DENV. CONCLUSION: As there is currently no approved vaccine for DENV, public health monitoring and a greater understanding of transmission dynamics leading to outbreak events are critical. The further characterization of DENV using this model will expand knowledge of key entomological, virological and immunological components of infection establishment and transmission events
The MAJORANA DEMONSTRATOR: A Search for Neutrinoless Double-beta Decay of Germanium-76
The {\sc Majorana} collaboration is searching for neutrinoless double beta
decay using Ge, which has been shown to have a number of advantages in
terms of sensitivities and backgrounds. The observation of neutrinoless
double-beta decay would show that lepton number is violated and that neutrinos
are Majorana particles and would simultaneously provide information on neutrino
mass. Attaining sensitivities for neutrino masses in the inverted hierarchy
region, meV, will require large, tonne-scale detectors with extremely
low backgrounds, at the level of 1 count/t-y or lower in the region of
the signal. The {\sc Majorana} collaboration, with funding support from DOE
Office of Nuclear Physics and NSF Particle Astrophysics, is constructing the
{\sc Demonstrator}, an array consisting of 40 kg of p-type point-contact
high-purity germanium (HPGe) detectors, of which 30 kg will be enriched
to 87% in Ge. The {\sc Demonstrator} is being constructed in a clean
room laboratory facility at the 4850' level (4300 m.w.e.) of the Sanford
Underground Research Facility (SURF) in Lead, SD. It utilizes a compact graded
shield approach with the inner portion consisting of ultra-clean Cu that is
being electroformed and machined underground. The primary aim of the {\sc
Demonstrator} is to show the feasibility of a future tonne-scale measurement in
terms of backgrounds and scalability.Comment: Proceedings for the MEDEX 2013 Conferenc
Contamination Control and Assay Results for the Majorana Demonstrator Ultra Clean Components
The MAJORANA DEMONSTRATOR is a neutrinoless double beta decay experiment
utilizing enriched Ge-76 detectors in 2 separate modules inside of a common
solid shield at the Sanford Underground Research Facility. The DEMONSTRATOR has
utilized world leading assay sensitivities to develop clean materials and
processes for producing ultra-pure copper and plastic components. This
experiment is now operating, and initial data provide new insights into the
success of cleaning and processing. Post production copper assays after the
completion of Module 1 showed an increase in U and Th contamination in finished
parts compared to starting bulk material. A revised cleaning method and
additional round of surface contamination studies prior to Module 2
construction have provided evidence that more rigorous process control can
reduce surface contamination. This article describes the assay results and
discuss further studies to take advantage of assay capabilities for the purpose
of maintaining ultra clean fabrication and process design.Comment: Proceedings of Low Radioactivity Techniques (LRT May 2017, Seoul
Status of the MAJORANA DEMONSTRATOR experiment
The MAJORANA DEMONSTRATOR neutrinoless double beta-decay experiment is
currently under construction at the Sanford Underground Research Facility in
South Dakota, USA. An overview and status of the experiment are given.Comment: 8 pages, proceeding from VII International Conference on
Interconnections between Particle Physics and Cosmology (PPC 2013), submitted
to AIP proceeding
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