Description of the diploid chromosome set of Triatoma pintodiasi (Hemiptera, Triatominae)

Submitted by Sandra Infurna ([email protected]) on 2016-12-08T14:53:03Z No. of bitstreams: 1 fff_moreira_etal_IOC_2016.pdf: 1477426 bytes, checksum: c1ae6f02b8d9c4162758b4084d57a2cd (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2016-12-08T14:59:48Z (GMT) No. of bitstreams: 1 fff_moreira_etal_IOC_2016.pdf: 1477426 bytes, checksum: c1ae6f02b8d9c4162758b4084d57a2cd (MD5)Made available in DSpace on 2016-12-08T14:59:48Z (GMT). No. of bitstreams: 1 fff_moreira_etal_IOC_2016.pdf: 1477426 bytes, checksum: c1ae6f02b8d9c4162758b4084d57a2cd (MD5) Previous issue date: 2016Universidade Estadual Paulista “Júlio de Mesquita Filho”. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho”. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Triatoma pintodiasi has been described and recently grouped in the Rubrovaria subcomplex. T. pintodiasi was initially compared to T. carcavalloi by staining and subsequently identified as T. circummaculata. However, after thorough examination, it was observed to be a cryptic species of T. circummaculata, and was described based on morphological features, morphometric data, and biochemical patterns of hemolymph. Thus, this paper aims to describe the karyotype of, and spermatogenesis in, T. pintodiasi, in order to elucidate the reproductive biology and taxonomy of the species. Sex chromosomes of T. pintodiasi formed a heteropyknotic chromocenter, and compaction of chromatin was observed during prophase. However, in contrast to observations in T. carcavalloi and T. circummaculata, in T. pintodiasi it was observed individualization of the sex chromosomes. The diploid chromosome set of the species 2n = 22 (20A + XY) is described through analysis of metaphases I and II. Initial cytogenetic characteristics of T. pintodiasi are described and the observed differences in the chromocenter are suggested as a possible cytotaxonomic tool. To gain a better understanding of the specific status of this cryptic species, however, we emphasize the need for further cytogenetic, molecular, biological, and biogeographical analysis, in addition to experimental hybrid crosses with other species of the Rubrovaria subcomplex

New evidence of the evolutionary relationship of the flavida complex with the genus Panstrongylus (Hemiptera, Triatominae) by karyosystematic

Submitted by Sandra Infurna ([email protected]) on 2018-10-04T11:34:56Z No. of bitstreams: 1 FFF_moreira_etal_IOC_2018.pdf: 729627 bytes, checksum: 3022397dc5eb63420c03f4e4599a05d4 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-10-04T11:43:47Z (GMT) No. of bitstreams: 1 FFF_moreira_etal_IOC_2018.pdf: 729627 bytes, checksum: 3022397dc5eb63420c03f4e4599a05d4 (MD5)Made available in DSpace on 2018-10-04T11:43:47Z (GMT). No. of bitstreams: 1 FFF_moreira_etal_IOC_2018.pdf: 729627 bytes, checksum: 3022397dc5eb63420c03f4e4599a05d4 (MD5) Previous issue date: 2018Universidade Estadual Paulista “Júlio de Mesquita Filho. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ. BrasilUniversidade Estadual Paulista “Júlio de Mesquita Filho. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil

Nucleolar activity during larval development of Myrmeleon uniformis Navas, 1920 (Neuroptera, Myrmeleontidae)

It has been reported in the literature that the Malpighian tubules of Neuroptera in the third instar undergo drastic histological changes, when they stop functioning in osmoregulation and start to secrete silk fibers for a cocoon. Therefore, to increase our knowledge about these cellular alterations that occur in the larvae of Neuroptera, we analyzed the cells that constitute the Malpighian tubules of each larval instar of the species Myrmeleon uniformis, with emphasis on nucleolar activity. Malpighian tubules, after being removed, were fixed on a slide using liquid nitrogen and stained by silver impregnation. In addition, total protein of the tubules was quantified. By analyzing the cells in the first instar larval stage, we observed only two silver-stained nucleolar regions. In cells of second instar larvae, there was an increase in the number of stained regions, and in the third instar, the number of nucleolar regions was very large. Agarose gel electrophoresis indicated that third instar larvae had high synthetic activity, where the total amount of proteins was larger in third instar stage than in the other larval stages. Furthermore, the most abundant proteins displayed molecular weights of about 32-43 kDa and were probably precursors of silk fibers. Thus, the results obtained showed that nucleolar alterations occur in the cells of the Malpighian tubules of larval instars of M. uniformis and this is directly related to the production of silk fibers used by the pupa to ensure the completion of metamorphosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Entoepidemiology of Chagas disease in the Western region of the State of Sao Paulo from 2004 to 2008, and cytogenetic analysis in Rhodnius neglectus (Hemiptera, Triatominae)

To complement the epidemiological data and assist in the prophylaxis of Chagas disease in the State of Sao Paulo, we examined entomological lifting conducted in 40 municipalities of the Western region of the state from 2004 to 2008, highlighted the main vector species in this region, and reanalyzed the cytogenetic characteristics of Rhodnius neglectus from 3 different Brazilian states (Formoso/GO, Frutal/MG, Guaira/SP, and Pitangueiras/SP). The municipalities of Castilho and Santo Antonio do Acarangua registered the highest relative amounts of notifications. The main species notified in Western Sao Paulo were Triatoma sordida and R. neglectus. We collected a large number of T. sordida in 2005 and noted the absence of notification of infected insects in 2008. We observed no variation in chromosomal characteristics of R. neglectus of different states. These data are complementary to the survey presented from 1990 to 1999, as the vector species were the same (T. sordida and R. neglectus), with emphasis on T. sordida. We corroborate the future colonization domiciliary initially proposed for T. sordida in the region and underscore the importance of vector control programs in the prophylaxis of Chagas disease. Furthermore, we observed that the populations of R. neglectus in Brazil showed no intraspecific variation and we corroborated the chromosomal patterns originally described. These data are important for understanding the evolution of these hematophagous insects, which are vectors of Chagas disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq