Spectral variation in the X-ray pulsar GX 1+4 during a low-flux episode

The X-ray pulsar GX 1+4 was observed with the RXTE satellite for a total of 51ks between 1996 July 19 - 21. During this period the flux decreased smoothly from an initial mean level of ~ 6 X 10^36 erg/s to a minimum of ~ 4 X 10^35 erg/s (2-60 keV, assuming a source distance of 10 kpc) before partially recovering towards the initial level at the end of the observation. BATSE pulse timing measurements indicate that a torque reversal took place approximately 10 d after this observation. Both the mean pulse profile and the photon spectrum varied significantly. The observed variation in the source may provide important clues as to the mechanism of torque reversals. The single best-fitting spectral model was based on a component originating from thermal photons with kT ~ 1 keV Comptonised by a plasma of temperature kT \~ 7 keV. Both the flux modulation with phase during the brightest interval and the evolution of the mean spectra over the course of the observation are consistent with variations in this model component; with, in addition, a doubling of the column density nH contributing to the mean spectral change. A strong flare of duration 50 s was observed during the interval of minimum flux, with the peak flux ~ 20 times the mean level. Although beaming effects are likely to mask the true variation in Mdot thought to give rise to the flare, the timing of a modest increase in flux prior to the flare is consistent with dual episodes of accretion resulting from successive orbits of a locally dense patch of matter in the accretion disc.Comment: 8 pages, 3 figures, submitted to MNRA

Perspectives in Cell Cycle Regulation: Lessons from an Anoxic Vertebrate

The ability of an animal, normally dependent on aerobic respiration, to suspend breathing and enter an anoxic state for long term survival is clearly a fascinating feat, and has been the focus of numerous biochemical studies. When anoxia tolerant turtles are faced with periods of oxygen deprivation, numerous physiological and biochemical alterations take place in order to facilitate vital reductions in ATP consumption. Such strategies include reversible post-translational modifications as well as the implementation of translation and transcription controls facilitating metabolic depression. Although it is clear that anoxic survival relies on the suppression of ATP consuming processes, the state of the cell cycle in anoxia tolerant vertebrates remain elusive. Several anoxia tolerant invertebrate and embryonic vertebrate models display cell cycle arrest when presented with anoxic stress. Despite this, the cell cycle has not yet been characterized for anoxia tolerant turtles. Understanding how vertebrates respond to anoxia can have important clinical implications. Uncontrollable cellular proliferation and hypoxic tumor progression are inescapably linked in vertebrate tissues. Consequentially, the molecular mechanisms controlling these processes have profound clinical consequences. This review article will discuss the theory of cell cycle arrest in anoxic vertebrates and more specifically, the control of the retinoblastoma pathway, the molecular markers of cell cycle arrest, the activation of checkpoint kinases, and the possibility of translational controls implemented by microRNAs

Angiogenic signaling in the lungs of a metabolically suppressed hibernating mammal (Ictidomys tridecemlineatus)

To conserve energy in times of limited resource availability, particularly during cold winters, hibernators suppress even the most basic of physiologic processes. Breathing rates decrease from 40 breaths/minute to less than 1 breath/min as they decrease body temperature from 37oC to ambient. Nevertheless, after months of hibernation, these incredible mammals emerge from torpor unscathed. This study was conducted to better understand the protective and possibly anti-inflammatory adaptations that hibernator lungs may use to prevent damage associated with entering and emerging from natural torpor. We postulated that the differential protein expression of soluble protein receptors (decoy receptors that sequester soluble ligands to inhibit signal transduction) would help identify inhibited inflammatory signaling pathways in metabolically suppressed lungs. Instead, the only two soluble receptors that responded to torpor were sVEGFR1 and sVEGFR2, two receptors whose full-length forms are bound by VEGF-A to regulate endothelial cell function and angiogenesis. Decreased sVEGFR1/2 correlated with increased total VEGFR2 protein levels. Maintained or increased levels of key ã-secretase subunits suggested that decreased sVEGFR1/2 protein levels were not due to decreased levels of intramembrane cleavage complex subunits. VEGF-A protein levels did not change, suggesting that hibernators may regulate VEGFR1/2 signaling at thes level of the receptor instead of increasing relative ligand

Pulse Profiles, Accretion Column Dips and a Flare in GX 1+4 During a Faint State

The Rossi X-ray Timing Explorer (RXTE) spacecraft observed the X-ray pulsar GX 1+4 for a period of 34 hours on July 19/20 1996. The source faded from an intensity of ~20 mCrab to a minimum of <~0.7 mCrab and then partially recovered towards the end of the observation. This extended minimum lasted ~40,000 seconds. Phase folded light curves at a barycentric rotation period of 124.36568 +/- 0.00020 seconds show that near the center of the extended minimum the source stopped pulsing in the traditional sense but retained a weak dip feature at the rotation period. Away from the extended minimum the dips are progressively narrower at higher energies and may be interpreted as obscurations or eclipses of the hot spot by the accretion column. The pulse profile changed from leading-edge bright before the extended minimum to trailing-edge bright after it. Data from the Burst and Transient Source Experiment (BATSE) show that a torque reversal occurred <10 days after our observation. Our data indicate that the observed rotation departs from a constant period with a Pdot/P value of ~-1.5% per year at a 4.5 sigma significance. We infer that we may have serendipitously obtained data, with high sensitivity and temporal resolution about the time of an accretion disk spin reversal. We also observed a rapid flare which had some precursor activity, close to the center of the extended minimum.Comment: 19 pages, 6 figures, accepted for publication in Astrophysical Journal (tentatively scheduled for vol. 529 #1, 20 Jan 2000

Navigating oxygen deprivation: Liver transcriptomic responses of the red eared slider turtle to environmental anoxia

The best facultative anaerobes among vertebrates are members of the genera Trachemys (pond slider turtles) and Chrysemys (painted turtles), and are able to survive without oxygen for up to 12 to 18 weeks at ∼3 °C. In this study, we utilized RNAseq to profile the transcriptomic changes that take place in response to 20 hrs of anoxia at 5 °C in the liver of the red eared slide turtle (Trachemys scripta elegans). Sequencing reads were obtained from at least 18,169 different genes and represented a minimum 49x coverage of the C. picta bellii exome. A total of 3,105 genes showed statistically significant changes in gene expression between the two animal groups, of which 971 also exhibited a fold change equal to or greater than 50% of control normoxic values. This study also highlights a number of anoxia-responsive molecular pathways that are may be important to navigating anoxia survival. These pathways were enriched in mRNA found to significantly increase in response to anoxia and included molecular processes such as DNA damage repair and metabolic reprogramming. For example, our results indicate that the anoxic turtle may utilize succinate metabolism to yield a molecule of GTP in addition to the two molecules that results from lactate production, and agrees with other established models of anoxia tolera

Aestivation: Signaling and hypometabolism

Aestivation is a survival strategy used by many vertebrates and invertebrates to endure arid environmental conditions. Key features of aestivation include strong metabolic rate suppression, strategies to retain body water, conservation of energy and body fuel reserves, altered nitrogen metabolism, and mechanisms to preserve and stabilize organs, cells and macromolecules over many weeks or months of dormancy. Cell signaling is crucial to achieving both a hypometabolic state and reorganizing multiple metabolic pathways to optimize long-term viability during aestivation. This commentary examines the current knowledge about cell signaling pathways that participate in regulating aestivation, including signaling cascades mediated by the AMPactivated kina

Bistability in a simple fluid network due to viscosity contrast

We study the existence of multiple equilibrium states in a simple fluid network using Newtonian fluids and laminar flow. We demonstrate theoretically the presence of hysteresis and bistability, and we confirm these predictions in an experiment using two miscible fluids of different viscosity--sucrose solution and water. Possible applications include bloodflow, microfluidics, and other network flows governed by similar principles

A New Look At Carbon Abundances In Planetary Nebulae. III. DDDM1, IC 3568, IC4593, NGC 6210, NGC 6720, NGC 6826, & NGC 7009

This paper is the third in a series reporting on a study of carbon abundances in a carefully chosen sample of planetary nebulae representing a large range in progenitor mass and metallicity. We make use of the IUE Final Archive database containing consistently-reduced spectra to measure line strengths of C III] 1909 along with numerous other UV lines for the planetary nebulae DDDM1, IC 3568, IC 4593, NGC 6210, NGC 6720, NGC 6826, & NGC 7009. We combine the IUE data with line strengths from optical spectra obtained specifically to match the IUE slit positions as closely as possible, to determine values for the abundance ratios He/H, O/H, C/O, N/O, and Ne/O. The ratio of C III] 1909/C II 4267 is found to be effective for merging UV and optical spectra when He II 1640/4686 is unavailable. Our abundance determination method includes a 5-level program whose results are fine-tuned by corrections derived from detailed photoionization models constrained by the same set of emission lines. All objects appear to have subsolar levels of O/H, and all but one show N/O levels above solar. In addition, the seven planetary nebulae span a broad range in C/O values. We infer that many of our objects are matter bounded, and thus the standard ionization correction factor for N/O may be inappropriate for these PNe. Finally, we estimate C/O using both collisionally-excited and recombination lines associated with C+2 and find the well established result that abundances from recombination lines usually exceed those from collisionally-excited lines by several times.Comment: 36 pages, 7 tables, 2 figures, latex. Tables and figures supplied as two separate postscript files. Accepted for publication in Ap

Mutual assessment in the social programmer ecosystem: an empirical investigation of developer profile aggregators

The multitude of social media channels that programmers can use to participate in software development has given rise to online developer profiles that aggregate activity across many services. Studying members of such developer profile aggregators, we found an ecosystem that revolves around the social programmer. Developers are assessing each other to evaluate whether other developers are interesting, worth following, or worth collaborating with. They are self-conscious about being assessed, and thus manage their public images. They value passion for software development, new technologies, and learning. Some recruiters participate in the ecosystem and use it to find candidates for hiring; other recruiters struggle with the interpretation of signals and issues of trust. This mutual assessment is changing how software engineers collaborate and how they advance their skills.Leif Singer, Fernando Figueira Filho, Brendan Cleary, Christoph Treude, Margaret-Anne Storey, Kurt Schneide