EFFECT OF LUTEIN IN HYPERCHOLESTEROLEMIA INDUCED OXIDATIVE STRESS IN MALE WISTAR RATS

Objective: Oxidative stress induced by reactive oxygen species (ROS) plays an important role in the etiology of several diseases, including atherosclerosis and coronary heart disease. Hypercholesterolemia is reported to be associated with the oxidative stress that results from the increased production of ROS or impairment of the antioxidant system. Hence the objective of this study was to evaluate the antioxidant effect of lutein in hypercholesterolemiainduced oxidative stress in male wistar rats. Methods: Male Wistar rats were divided into 6 groups of 6 each. Group I served as control. Group II III, IV, V & VI rats were received high cholesterol diet. Group III was treated with Atorvastatin 5 mg/kg. Group IV, V & VI rats was treated with 25 mg/kg, 50 mg/kg & 100 mg/kg of Lutein. After 16 weeks, blood samples &liver tissue samples were collected from all the groups of animals to evaluate antioxidant levels in blood & tissue samples. Results: Catalase, superoxide dismutase, glutathione peroxidase, glutathione levels significantly increased in both plasma & liver in lutein treated groups. TBARs level is increased in plasma in hypercholesterolemicrats and Malondialdehyde level in the liver tissue is also significantly increased in hypercholesterolemic rats. Conclusion: The results of this study indicate Lutein is an effective nutritional supplement to prevent oxidative stress in hypercholesterolemia

Effect of Lutein in the expression of PPARα and LDLR in hypercholesterolemic male Wistar Rats

Background: Hyperlipidemia is a well known risk factor for cardiovascular disease, especially atherosclerotic coronary artery disease. Peroxisome proliferator activated receptor α (PPARα), a member of this nuclear receptor family, has emerged as an important player in this scenario, with evidence supporting a central co-ordinated role in the regulation of fatty acid oxidation, lipid and lipoprotein metabolism and inflammatory and vascular responses, all of which would be predicted to reduce atherosclerotic risk. The low-density lipoprotein (LDL) receptor (LDLR) is the primary pathway for removal of cholesterol from the circulation, and its activity is meticulously governed by intracellular cholesterol levels. Hence in this study we investigated the effect of Lutein on PPARα and LDLR expression in liver of wistar rats.Methods: Male Wistar rats were divided into 6 groups of 6 each. Group I served as control. Group II III, IV, V and VI rats were received high cholesterol diet. Group III was treated with Atorvastatin 5mg/kg. Group IV, V and VI rats were treated with 25mg/kg, 50mg/kg and 100mg/kg of Lutein. After 16 weeks, liver tissue samples were collected from all the groups of animals to evaluate the expression of PPARα and LDLR.Results: The expression of Peroxisome proliferator activated receptor α and low-density lipoprotein (LDL) receptor (LDLR) was significantly increased in Lutein treated hypercholesterolemic male wistar rats.Conclusions: The results of this study indicate that Lutein activates LDL receptor and PPARα in hypercholesterolemic male wistar rats

A randomized trial comparing the efficacy of oral preemptive pregabalin and gabapentin for postoperative analgesia

Background: Modern anesthesia practice utilizes preemptive analgesia for providing pain relief postoperatively using oral drugs such as pregabalin and gabapentin which are otherwise used for chronic pain management. We aimed to determine which among the two is more proficient. Materials and Methods: This randomized double-blinded control study was done among 72 subjects undergoing lower abdominal surgeries under the subarachnoid block and they were randomly divided into three groups: Group 1 received a single dose of tablet pregabalin 150 mg orally, Group 2 received tablet gabapentin 600 mg orally, and Group 3 received placebo drug orally (tablet B-complex) 2 h before subarachnoid block. Pain scores, time of 1st rescue analgesic, total number of rescue analgesics, sedation score, and occurrence of side effects were also noted for 24 h. The data were analyzed using SPSS 16 with Chi-square/Fisher's exact test or analysis of variance/Kruskal–Wallis test. A statistically significant difference was considered when P < 0.05. Results: Pain scores were lesser in Group 1 and 2 except at 2nd h. Total rescue analgesic time among maximum subjects in Group 1 was 4 h 30 min, in Group 2 was 3 h 30 min and 4 h, whereas in Group 3 was 3 h (P < 0.001). The total dose of analgesics in the first 24 h was 2 doses in Group 1, 2 doses in Group 2, and 3 doses in Group 3 (P < 0.001). Sedation score was more on Group 1 and 2 in comparison to Group 3 with no side effects in all groups. Conclusions: We conclude that pregabalin to be the effective preemptive drug because of its lesser pain intensity scores and good sedation property

Effect of Lutein in the expression of PPARα and LDLR in hypercholesterolemic male Wistar Rats

Background: Hyperlipidemia is a well known risk factor for cardiovascular disease, especially atherosclerotic coronary artery disease. Peroxisome proliferator activated receptor α (PPARα), a member of this nuclear receptor family, has emerged as an important player in this scenario, with evidence supporting a central co-ordinated role in the regulation of fatty acid oxidation, lipid and lipoprotein metabolism and inflammatory and vascular responses, all of which would be predicted to reduce atherosclerotic risk. The low-density lipoprotein (LDL) receptor (LDLR) is the primary pathway for removal of cholesterol from the circulation, and its activity is meticulously governed by intracellular cholesterol levels. Hence in this study we investigated the effect of Lutein on PPARα and LDLR expression in liver of wistar rats.Methods: Male Wistar rats were divided into 6 groups of 6 each. Group I served as control. Group II III, IV, V and VI rats were received high cholesterol diet. Group III was treated with Atorvastatin 5mg/kg. Group IV, V and VI rats were treated with 25mg/kg, 50mg/kg and 100mg/kg of Lutein. After 16 weeks, liver tissue samples were collected from all the groups of animals to evaluate the expression of PPARα and LDLR.Results: The expression of Peroxisome proliferator activated receptor α and low-density lipoprotein (LDL) receptor (LDLR) was significantly increased in Lutein treated hypercholesterolemic male wistar rats.Conclusions: The results of this study indicate that Lutein activates LDL receptor and PPARα in hypercholesterolemic male wistar rats

Efficacy of fentanyl versus clonidine as an adjuvant to ropivacaine compared to ropivacaine for postoperative analgesia in caudal block for infraumblical pediatric surgeries: A prospective double-blinded randomized control study

Background: Postoperative pain and its management are a budding specialty in the medical field. The additions of adjuvants to local anesthetics were one of the effective methods to improve postoperative analgesia in terms of duration and profile. The study was done primarily to assess the duration of analgesia in terms of pain score and total analgesic requirement among fentanyl and clonidine. Materials and Methods: The study was a double-blinded randomized control trial among 72 pediatric subjects where the subjects were randomly allocated into: Fentanyl group: received 0.75 ml/kg ropivacaine 0.2% along with fentanyl 1 μg/kg, clonidine group received 0.75 ml/kg ropivacaine 0.2% along with clonidine 1 μg/kg and ropivacaine alone group received 0.75 ml/kg 0.2% ropivacaine alone. Pain was assessed by objective pain scale score and Ramsay Sedation Scale was used to assess postoperative sedation. The data were entered into Microsoft Excel and analyzed using SPSS 16. Results: Clonidine group showed bradycardia and hypotension intraoperatively and postoperatively as compared to the other groups (P < 0.05). Both the fentanyl and clonidine group showed increased sedation (3.13 [0.34] vs. 2.13 [0.54] and 1.96 [0.36]) longer duration of analgesia (15.50 [1.35], 11.67 [3.71] and 9.17 [1.86]) and lesser pain score compared to the placebo group (P < 0.05). Among the groups, clonidine showed a better profile. Conclusion: The study concluded that both fentanyl and clonidine as an adjuvant to ropivacaine showed longer duration of analgesia with increased sedation score and lesser pain score. Clonidine showed a better profile in terms of duration of analgesia, pain, and sedation