Skin-stringer separation in post-buckling of butt-joint stiffened thermoplastic composite panels

Two aeronautical thermoplastic composite stiffened panels are analysed and tested to investigate the buckling behaviour, the skin-stringer separation and the final failure mode. The panels are made of fast crystallising polyetherketoneketone carbon composite, have three stringers with an angled cap on one side, and are joined to the skin by a short-fibre reinforced butt-joint. The panels contain an initial damage in the middle skin-stringer interface representing barely visible impact damage. Finite element analysis using the virtual crack closure technique are conducted before the test to predict the structural behaviour. During the tests, the deformation of the panels is measured by digital image correlation, the damage propagation is recorded by GoPro cameras and the final failure is captured by high speed cameras. The panels show an initial three half-wave buckling shape in each bay, with damage propagation starting shortly after buckling. A combination of relatively stable and unstable damage propagation is observed until final failure, when the middle stringer separates completely and the panels fail in an unstable manner. The test results are compared to the numerical prediction, which shows great agreement for both the buckling and failure behaviour

Biological Signature of Scotian Shelf Water Crossovers on Georges Bank During Spring 1997

Episodic crossovers of cold low salinity Scotian Shelf Water (SSW) onto the Northeast Peak of Georges Bank are a potentially important mechanism transporting plankton species, including the copepod Calanus finmarchicus and its prey and predators, onto the Bank each spring. We provide the first detailed investigation of horizontal and vertical zooplankton distributions in SSW crossovers compared to other onbank locations from three GLOBEC cruises during spring 1997. SSW crossovers are physically and biologically distinct from other Bank locations. In late spring, chlorophyll concentrations and in vivo fluorescence are elevated and light transmission is reduced in SSW, while during early spring, these parameters are more variable. SSW communities do not contain a unique zooplankton assemblage or indicator species but instead show differences in abundance and life history parameters for various taxa compared to other Bank locations. SSW has high abundances of young C. finmarchicus life history stages, almost no diel vertical migration of zooplankton, low abundances of invertebrate predators, and low fish egg abundance. Population development of C. finmarchicus in SSW lags that in adjacent water. The potential biological impact of SSW crossovers on Georges Bank varies seasonally. In April, density inversions and interleaving of SSW and non‐SSW suggest active mixing, resulting in similar community composition of SSW and adjacent non‐SSW. SSW crossovers are probably an important source to Georges Bank of young stages of C. finmarchicus in early spring. In May, after stratification strengthens, the greater differentiation between SSW plankton and elsewhere indicates that mixing between communities is more limited

Empirical model for mean temperature for Indian zone and estimation of precipitable water vapor from ground based GPS measurements

Estimation of precipitable water (PW) in the atmosphere from ground-based Global Positioning System (GPS) essentially involves modeling the zenith hydrostatic delay (ZHD) in terms of surface Pressure (<I>P<sub>s</sub></I>) and subtracting it from the corresponding values of zenith tropospheric delay (ZTD) to estimate the zenith wet (non-hydrostatic) delay (ZWD). This further involves establishing an appropriate model connecting PW and ZWD, which in its simplest case assumed to be similar to that of ZHD. But when the temperature variations are large, for the accurate estimate of PW the variation of the proportionality constant connecting PW and ZWD is to be accounted. For this a water vapor weighted mean temperature (<I>T<sub>m</sub></I>) has been defined by many investigations, which has to be modeled on a regional basis. For estimating PW over the Indian region from GPS data, a region specific model for <I>T<sub>m</sub></I> in terms of surface temperature (<I>T<sub>s</sub></I>) is developed using the radiosonde measurements from eight India Meteorological Department (IMD) stations spread over the sub-continent within a latitude range of 8.5°–32.6° N. Following a similar procedure <I>T<sub>m</sub></I>-based models are also evolved for each of these stations and the features of these site-specific models are compared with those of the region-specific model. Applicability of the region-specific and site-specific <I>T<sub>m</sub></I>-based models in retrieving PW from GPS data recorded at the IGS sites Bangalore and Hyderabad, is tested by comparing the retrieved values of PW with those estimated from the altitude profile of water vapor measured using radiosonde. The values of ZWD estimated at 00:00 UTC and 12:00 UTC are used to test the validity of the models by estimating the PW using the models and comparing it with those obtained from radiosonde data. The region specific <I>T<sub>m</sub></I>-based model is found to be in par with if not better than a similar site-specific <I>T<sub>m</sub></I>-based model for the near equatorial station, Bangalore. A simple site-specific linear relation without accounting for the temperature effect through <I>T<sub>m</sub></I> is also found to be quite adequate for Bangalore. But for Hyderabad, a station located at slightly higher latitude, the deviation for the linear model is found to be larger than that of the <I>T<sub>m</sub></I>-based model. This indicates that even though a simple linear regression model is quite adequate for the near equatorial stations, where the temperature variations are relatively small, for estimating PW from GPS data at higher latitudes this model is inferior to the <I>T<sub>m</sub></I>-based model

Neoadjuvant chemotherapy and trastuzumab versus neoadjuvant chemotherapy followed by post-operative trastuzumab for patients with HER2-positive breast cancer

Neoadjuvant chemotherapy plus trastuzumab (NCT) increases the rate of pathological complete response (pCR) and event-free survival (EFS) compared to neoadjuvant chemotherapy (NC) alone in women with HER2 positive breast cancer (BC). pCR in this setting is associated with improved EFS. Whether NCT preferentially improves EFS in comparison to NC followed by adjuvant trastuzumab initiated postoperatively (NCAT) has not been addressed. Using clinical data from women with HER2 positive BC treated at 7 European institutions between 2007 and 2010 we sought to investigate the impact on breast cancer outcomes of concomitant (NCT) versus sequential (NCAT) treatment in HER2 positive early BC. The unadjusted hazard ratio (HR) for event free survival with NCT compared with NCAT was 0.63 (95% CI 0.37–1.08; p = 0.091). Multivariable analysis revealed that treatment group, tumour size and ER status were significantly associated with EFS from diagnosis. In the whole group NCT was associated with a reduced risk of an event relative to NCAT, an effect that was confined to ER negative (HR: 0.25; 95% CI, 0.10–0.62; p = 0.003) as opposed to ER positive tumours (HR: 1.07; 95% CI, 0.46–2.52; p = 0.869). HER2 positive/ER negative BC treated with NC gain greatest survival benefit when trastuzumab is administered in both the neoadjuvant and adjuvant period rather than in the adjuvant period alone. These data support the early introduction of targeted combination therapy in HER2 positive/ER negative BC

Poly purine.pyrimidine sequences upstream of the beta-galactosidase gene affect gene expression in Saccharomyces cerevisiae

BACKGROUND: Poly purine.pyrimidine sequences have the potential to adopt intramolecular triplex structures and are overrepresented upstream of genes in eukaryotes. These sequences may regulate gene expression by modulating the interaction of transcription factors with DNA sequences upstream of genes. RESULTS: A poly purine.pyrimidine sequence with the potential to adopt an intramolecular triplex DNA structure was designed. The sequence was inserted within a nucleosome positioned upstream of the β-galactosidase gene in yeast, Saccharomyces cerevisiae, between the cycl promoter and gal 10Upstream Activating Sequences (UASg). Upon derepression with galactose, β-galactosidase gene expression is reduced 12-fold in cells carrying single copy poly purine.pyrimidine sequences. This reduction in expression is correlated with reduced transcription. Furthermore, we show that plasmids carrying a poly purine.pyrimidine sequence are not specifically lost from yeast cells. CONCLUSION: We propose that a poly purine.pyrimidine sequence upstream of a gene affects transcription. Plasmids carrying this sequence are not specifically lost from cells and thus no additional effort is needed for the replication of these sequences in eukaryotic cells