12 research outputs found
Anisotropic cryostructured collagen scaffolds for efficient delivery of RhBMPâ2 and enhanced bone regeneration
In the treatment of bone non-unions, an alternative to bone autografts is the use of bone
morphogenetic proteins (BMPs), e.g., BMPâ2, BMPâ7, with powerful osteoinductive and osteogenic
properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges
for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological
doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and
excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed
cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of
cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic).
We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the
scaffolds by increasing the regenerative performance of the provided rhBMPâ2. In vitro, both scaffolds
presented similar mechanical properties, rhBMPâ2 retention and delivery capacity, as well as scaffold
degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in
a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic
cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMPâ2
mediated bone healing
Anisotropic cryostructured collagen scaffolds for efficient delivery of RhBMPâ2 and enhanced bone regeneration
In the treatment of bone non-unions, an alternative to bone autografts is the use of bone
morphogenetic proteins (BMPs), e.g., BMPâ2, BMPâ7, with powerful osteoinductive and osteogenic
properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges
for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological
doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and
excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed
cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of
cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic).
We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the
scaffolds by increasing the regenerative performance of the provided rhBMPâ2. In vitro, both scaffolds
presented similar mechanical properties, rhBMPâ2 retention and delivery capacity, as well as scaffold
degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in
a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic
cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMPâ2
mediated bone healing