\u3ci\u3eChildhood\u27s End\u3c/i\u3e

Each yesterday Ended when, quite magically, The streetlights blinked, and once again Bewitched the night

\u3ci\u3eConfessions of A Dragon Lady\u3c/i\u3e / \u3ci\u3eWindbound\u3c/i\u3e

Confessions of A Dragon Lady: Before I knew the wisdom of this world I felt the ebb and flow of secret tides. Windbound: Back of the North Wind, Under a pallid sun, all life Is permafrosted

Production of callus from protoplasts of cultured grape pericarp

Protoplasts were isolated from callus cultures of grape vine pericarp after an overnight incubation at 26 °c in 2% Cellulase Onozuka SS plus 1 % Macerozyme dissolved in a mixture of 0.14 molal KCl and 0.10 molal CaCl2 at pH 5.5. When cultured in a liquid medium with either 0.24 M sorbitol or 0.23 M sucrose as osmotic stabilizer, new cell walls were regenerated within the first few days. Cell division commenced by the sixth day of culture, and approximately 0.5% of the population continued dividing. Eventual transfer of the culture to the surface of medium solidified with agar resulted in callus formation.Kallusbildung aus Protoplasten von kultiviertem TraubenperikarpEine Nährlösung wird beschrieben, in der Protoplasten, die aus Kalluskulturen von Traubenperikarp isoliert worden waren, neue Zellwände bildeten und Zellteilungen eingingen. Spätere Übertragung auf einen festen Nährboden ergab aktives Kalluswachstum

In vitro propagation of grapevine (Vitis vinifera L.) from fragmented shoot apices

A method is described for the in vitro propagation of grapevine from fragmented shoot apices, which has the potential to produce approximately 8000 plantlets from a single apex within 4 months. Apical cell clumps were grown in a liquid culture medium with cytokinin but in the absence of auxin. Transfer of the differentiated cell clumps to the same medium gelled with agar resulted in shoot masses which could be repeatedly subcultured. Excised shoots rooted readily on a hormone-free basal medium, and were successfully transferred to glasshouse conditions. This method has potential value in commercial clonal grapevine propagation.In-vitro-Vermehrung der Rebe (Vitis vinifera L.) aus zerstückelten TriebspitzenEine Methode für die in-vitro-Vermehrung der Rebe aus zerstückelten Triebspitzen wird beschrieben. Zellklumpen der Triebspitze wurden auf einem flüssigen Medium, das Cytokinin, aber kein Auxin enthielt, kultiviert. Nach Übertragung der differenzierten Zellklumpen auf ein mit Agar verfestigtes, aber sonst gleich zusammengesetztes Medium entstanden Büschel von Trieben, die zerteilt und weiter vermehrt werden konnten. Explantierte Einzeltriebe bewurzelten sich auf einem hormonfreien Grundmedium rasch

The Cannon Hall Muscat grape

The Canon Hall Muscat table grape grown for export in Western Australia is consistent with the early descriptions and the present appearance of the original  Cannon Hall Muscat vine, and is clearly the same variety. It has the diploid number of chromosomes and the use of its name for tetraploid sports of Muscat of Alexandria is not justified.Die Rebensorte Cannon Hall MuscatDie Tafeltraube Canon Hall Muscat, die in Westaustralien zum Export angebaut wird, gleicht der ursprünglichen Cannon-Hall-Muscat-Rebe, da die erstgenannte eindeutig den alten Beschreibungen und dem heutigen Aussehen der letzteren entspricht. Canon Hall Muscat ist diploid, und deshalb sollte dieser Name nicht für tetraploide Mutanten von Muscat of Alexandria gebraucht werden

Partial characterization of Agrobacterium vitis strains

Seventeen strains of Agrobacterium vitis (formerly classified A. tumefaciens biovar 3) were characterized using part of the T-DNA and virA regions of the Ti plasmid as probes. All strains except one were of the wide host range (WHR) strains and were classified into two groups depending on their ability to utilize octopine or nopaline. These WHR type oncogenic strains had homology with the limited host range type (LHR) virA gen of A. vitis but not with the WHR virA gene of A. tumefaciens. The frequency of T-DNA excision in some Agrobacterium strains was estimated with the plasmid pTMA which mimics T-DNA excision from Ti plasmid DNA. In an A. vitis strain isolated from grapevine, T-DNA excision occurred after co-cultivation with grapevine tissues, but not with acetosyringone. In contrast, in A. tumefaciens, T-DNA excision occurred after co-cultivation with acetosyringone, but not with grapevine tissue

Ploidy stability in grapevines following long term storage in vitro

A modified method to prepare root tip squashes was used for the routine examination of chromosomes of grapevines developed from in vitro shoot cultures. The results established that adventitious buds obtained by culture of fragmented shoot apices were diploid, and that storage at 9.5 °C of multiple shoot cultures of eight Vitis genotypes for periods up to 12 months did not affect the ploidy level of plants regenerated from them