miR-100-5p inhibition induces apoptosis in dormant prostate cancer cells and prevents the emergence of castration-resistant prostate cancer

Carcinoma of the prostate is the most common cancer in men. Treatment of aggressive prostate cancer involves a regiment of radical prostectomy, radiation therapy, chemotherapy and hormonal therapy. Despite significant improvements in the last decade, the treatment of prostate cancer remains unsatisfactory, because a significant fraction of prostate cancers develop resistance to multiple treatments and become incurable. This prompts an urgent need to investigate the molecular mechanisms underlying the evolution of therapy-induced resistance of prostate cancer either in the form of castration-resistant prostate cancer (CRPC) or transdifferentiated neuroendocrine prostate cancer (NEPC). By analyzing micro-RNA expression profiles in a set of patient-derived prostate cancer xenograft tumor lines, we identified miR-100-5p as one of the key molecular components in the initiation and evolution of androgen ablation therapy resistance in prostate cancer. In vitro results showed that miR-100-5p is required for hormone-independent survival and proliferation of prostate cancer cells post androgen ablation. In Silico target predictions revealed that miR-100-5p target genes are involved in key aspects of cancer progression, and are associated with clinical outcome. Our results suggest that mir-100-5p is a possible therapeutic target involved in prostate cancer progression and relapse post androgen ablation therapy

Using Polarized Spectroscopy to Investigate Order in Thin-Films of Ionic Self-Assembled Materials Based on Azo-Dyes

Three series of ionic self-assembled materials based on anionic azo-dyes and cationic benzalkonium surfactants were synthesized and thin films were prepared by spin-casting. These thin films appear isotropic when investigated with polarized optical microscopy, although they are highly anisotropic. Here, three series of homologous materials were studied to rationalize this observation. Investigating thin films of ordered molecular materials relies to a large extent on advanced experimental methods and large research infrastructure. A statement that in particular is true for thin films with nanoscopic order, where X-ray reflectometry, X-ray and neutron scattering, electron microscopy and atom force microscopy (AFM) has to be used to elucidate film morphology and the underlying molecular structure. Here, the thin films were investigated using AFM, optical microscopy and polarized absorption spectroscopy. It was shown that by using numerical method for treating the polarized absorption spectroscopy data, the molecular structure can be elucidated. Further, it was shown that polarized optical spectroscopy is a general tool that allows determination of the molecular order in thin films. Finally, it was found that full control of thermal history and rigorous control of the ionic self-assembly conditions are required to reproducibly make these materials of high nanoscopic order. Similarly, the conditions for spin-casting are shown to be determining for the overall thin film morphology, while molecular order is maintained

Letter from G[rove] K[arl] Gilbert to John Muir, 1905 Apr 7.

[letterhead]April 7, 1905My dear Mr MuirLearning from Dr. Merriam that you are writing on the yosemites, it occurred to me that you might find some of my photographs useful - for illustration. Here are a few of them, including one little glacier. I have a number of views also showing glacial sculpture above timber.Cordially YoursG. K. Gilbert03554https://scholarlycommons.pacific.edu/jmcl/28456/thumbnail.jp

Letter from G[rove] K[arl] Gilbert to John Muir, 1905 Apr 7.

[letterhead]April 7, 1905My dear Mr MuirLearning from Dr. Merriam that you are writing on the yosemites, it occurred to me that you might find some of my photographs useful - for illustration. Here are a few of them, including one little glacier. I have a number of views also showing glacial sculpture above timber.Cordially YoursG. K. Gilbert03554https://scholarlycommons.pacific.edu/jmcl/28456/thumbnail.jp

Letter from G[rove] K[arl] Gilbert to John Muir, 1899 Sep 3.

[1]Portland, Sept. 8, 1899My dear Mr. MuirIt naturally gave me pain to note that your abiding forth in glaciers seemed to leave little room for faith of another order and I resolved to apply the antidote at the earliest opportunity. There will come to you by mail from the publisher a little volume entitled Inquirendo Island , for which I bespeak your warmest attention. as a lady recently wrote me regarding Sartor02616[2]resartus , you should so far as possible empty your mind of preconceptions when you open the book so as to approach it in a condition of childlike reciprocity - and let it sink into your soul, so to speak.I had hoped to reach Martinez at about the same time as the book but am detained by some shadowy glaciers that refuse to declare them positively as either myths or veritable02616[3]shades of the departed.However my hope is merely deferred not surrendered, and I shall follow this line in a few days.Sincerely YoursG.K. Gilbert02616https://scholarlycommons.pacific.edu/jmcl/27182/thumbnail.jp

Letter from G[rove] K[arl] Gilbert to John Muir, 1899 Sep 3.

[1]Portland, Sept. 8, 1899My dear Mr. MuirIt naturally gave me pain to note that your abiding forth in glaciers seemed to leave little room for faith of another order and I resolved to apply the antidote at the earliest opportunity. There will come to you by mail from the publisher a little volume entitled Inquirendo Island , for which I bespeak your warmest attention. as a lady recently wrote me regarding Sartor02616[2]resartus , you should so far as possible empty your mind of preconceptions when you open the book so as to approach it in a condition of childlike reciprocity - and let it sink into your soul, so to speak.I had hoped to reach Martinez at about the same time as the book but am detained by some shadowy glaciers that refuse to declare them positively as either myths or veritable02616[3]shades of the departed.However my hope is merely deferred not surrendered, and I shall follow this line in a few days.Sincerely YoursG.K. Gilbert02616https://scholarlycommons.pacific.edu/jmcl/27182/thumbnail.jp