Library Resources and Services of Selected Deemed University Libraries in Andhra Pradesh, India

This paper defines the role of selected Deemed University libraries in Andhra Pradesh, Indian in higher education and research of Engineering, Science and technology. This paper systematically studies the operational, the resources and services of these libraries. The collection development, library membership, staff position, working hours, library automation, services offered and availability of online resources are also discussed. Keywords: Deemed University libraries, Academic libraries, Library resources and services, Library automation, Andhra Prades

Novel magnetic properties of graphene: Presence of both ferromagnetic and antiferromagnetic features and other aspects

Investigations of the magnetic properties of graphenes prepared by different methods reveal that dominant ferromagnetic interactions coexist along with antiferromagnetic interactions in all the samples. Thus, all the graphene samples exhibit room-temperature magnetic hysteresis. The magnetic properties depend on the number of layers and the sample area, small values of both favoring larger magnetization. Molecular charge-transfer affects the magnetic properties of graphene, interaction with a donor molecule such as tetrathiafulvalene having greater effect than an electron-withdrawing molecule such as tetracyanoethyleneComment: 16 pges, 5 figure

Purification and characterization of cysteine protease from germinating cotyledons of horse gram

<p>Abstract</p> <p>Background</p> <p>Proteolytic enzymes play central role in the biochemical mechanism of germination and intricately involved in many aspects of plant physiology and development. To study the mechanism of protein mobilization, undertaken the task of purifying and characterizing proteases, which occur transiently in germinating seeds of horse gram.</p> <p>Results</p> <p>Cysteine protease (CPRHG) was purified to homogeneity with 118 fold by four step procedure comprising Crude extract, (NH4)2SO4 fractionation, DEAE-Cellulose and CM-sephacel chromatography from the 2 day germinating cotyledons of horse gram (<it>Macrotyloma uniflorum </it>(Lam.) Verdc.). CPRHG is a monomer with molecular mass of 30 k Da, was determined by SDS-PAGE and gel filtration. The purified enzyme on IEF showed two isoforms having pI values of 5.85 and 6.1. CPRHG composed of high content of aspartic acid, glutamic acid and serine. The enzyme activity was completely inhibited by pCMB, iodoacetate and DEPC indicating cysteine and histidine residues at the active site. However, on addition of sulfhydryl reagents (cysteine, dithiothreitol, glutathione and beta-ME) reverse the strong inhibition by pCMB. The enzyme is fairly stable toward pH and temperature. Immunoblot analysis shows that the enzyme synthesized as zymogen (preproenzyme with 81 kDa) and processed to a 40 kDa proenzyme which was further degraded to give 30 kDa active enzyme.</p> <p>Conclusion</p> <p>It appears that the newly synthesized protease is inactive, and activation takes place during germination. CPRHG has a broad substrate specificity and stability in pH, temperature, etc. therefore, this protease may turn out to be an efficient choice for the pharmaceutical, medicinal, food, and biotechnology industry.</p

Quenching of fluorescence of aromatic molecules by graphene due to electron transfer

Investigations on the fluorescence quenching of graphene have been carried out with two organic donor molecules, pyrene butanaoic acid succinimidyl ester (PyBS, I) and oligo(p-phenylenevinylene) methyl ester (OPV-ester, II). Absorption and photoluminescence spectra of I and II recorded in mixture with increasing the concentrations of graphene showed no change in the former, but remarkable quenching of fluorescence. The property of graphene to quench fluorescence of these aromatic molecules is shown to be associated with photo-induced electron transfer, on the basis of fluorescence decay and time-resolved transient absorption spectroscopic measurements.Comment: 18 pages, 6 figure