Isolation and cellular fatty acid profile analyzation of two marine bioluminescent bacteria

192-195Two luminescent bacterial strains KOOS1 and KOOS2 isolated from surface mucus of Octopus sp. collected from Andaman were identified by their cellular fatty acid composition analyzation with the help of Microbial Identification system (MIDI). SIM indexes obtained for these isolated strains were 0.772 (KOOS1) and 0.754 (KOOS2) respectively and were identified as Photobacterium damselae and Vibrio fischeri. Major fatty acids found in Photobacterium damselae were Saturated: Dodecanoic acid (C12:0), Tetradecanoic acid (C14:0), Pentadecanoic acid (C15:0), Hexadecanoic acid (C16:0), Heptadecanoic acid (C17:0) and Octadecanoic acid (C18:0); and Unsaturated: 3-hydroxy-9-methyl decanoic acid (C11:0iso 3OH), 3-hydroxydodecanoic(C12:0 3OH), C16:1ω5c, Oleic acid (C18:1ω9c) and C18:1ω5c.In Vibrio fischeri Saturated: C12:0, Tridecanoic acid (C13:0), C15:0, C16:0, C17:0 and C18:0; and Unsaturated: C11:0iso 3OH,2-hydroxydodecanoic (C12:0 2OH), C12:03OH, C13:0iso, C14:0iso, C15:0iso, C15:0anteiso, C16:0iso, C17:0iso, C16:1ω5c, C15:0iso3OH, C17:1 ω8c and C17:1ω6c were found. Cyclopropane acids have not been detected in both Photobacterium damselae and Vibrio fischeri

Assessment of antibacterial efficacy of the methanolic extract of Bacillus vietnamensis PBChS1 isolated from marine sediments of Chidiyatapu coast, South Andaman

1009-1017Marine bacteria are of extensive significance as new ‘budding springs’ of a vast number of organic active artefacts. Till now, only a fraction of microorganisms have been scrutinized for bioactive metabolites, yet a colossal sum of these metabolites with unique structural drafts remains untouched and unexploited. In this respect, the species of Bacillus vietnamensis was isolated from the marine sediments of Chidiyatapu coast, South Andaman and characterized by both phenotypic and genotypic methods. The anti-oxidative activities of the methanolic extract were determined by the estimation of TPC (Total Phenolic Content), reducing power, free radical scavenging assay and ferric thiocyanate method. Antibacterial activity of the methanolic extract was analyzed by an agar well diffusion assay confirming the presence of active metabolites of various functional groups by FT-IR, thereby warranting a ‘combinatorial approach’ for applicative pharmacological settings

Assessment of antibacterial efficacy of the methanolic extract of Bacillus vietnamensis PBChS1 isolated from marine sediments of Chidiyatapu coast, South Andaman

Marine bacteria are of extensive significance as new ‘budding springs’ of a vast number of organic active artefacts. Till now, only a fraction of microorganisms have been scrutinized for bioactive metabolites, yet a colossal sum of these metabolites with unique structural drafts remains untouched and unexploited. In this respect, the species of Bacillus vietnamensis was isolated from the marine sediments of Chidiyatapu coast, South Andaman and characterized by both phenotypic and genotypic methods. The anti-oxidative activities of the methanolic extract were determined by the estimation of TPC (Total Phenolic Content), reducing power, free radical scavenging assay and ferric thiocyanate method. Antibacterial activity of the methanolic extract was analyzed by an agar well diffusion assay confirming the presence of active metabolites of various functional groups by FT-IR, thereby warranting a ‘combinatorial approach’ for applicative pharmacological settings

Distribution of bioluminescent polychaete larvae of <i>Odontosyllis</i> sp. in South Andaman

735-737Larval distribution of a luminous Odontosyllis sp. on different marine niches such as the articulated coralline red algae Amphirova anceps, algal mats on boats, seawater and sediment is being reported here for the first time from Burmanallah Coast.The freshly collected luminous larvae of Odontosyllis sp. from these niches emitted an intense glow of luminescence upon the addition of freshwater and upon gentle disturbance. Flashes of internal luminescence was appeared in freshly collected larvae, whereas continuous glow lasted for few minutes when freshwater was added. Luminescent bacteria were not observed in these larvae, indicating that their luminescence emission is not due to bacterial production, but could be the self-internal emission mechanism

Polymorphic mantle tissue patterns of <i>Tridacna</i> of Burmanallah coast, South Andaman

141-146We investigated 42 different mantle tissue patterns in Tridacna species along the coast of Burmanallah, Andamans. Out of 42 mantle tissue patterns, T. maxima showed 18 different mantle patterns, T. crocea showed 22 different mantle patterns and T. squamosa showed only two patterns. T. maxima showed dominance of distribution than T. crocea and T. squamosa. Mostly T. maxima and T. crocea were observed in shallow waters and T. squamosa was present on the slope edge of reefs

Molecular characterization of marine pigmented bacteria showing antibacterial activity

2081-2087In the present study 6 pigmented marine bacterial (PMB) strains BNO10, BNO20, BSO14, BNY11, CSP15 and BNB21 from marine sediment and 2 strains BWR18 and BWCY16 from seawater sample were isolated on Zobell Marine agar. These PMB appeared to be in blue, orange, pink, red, dark brownish yellow and yellowish green in colour. Phylogenetic analysis on the basis of small subunit ribosomal RNA (16s rRNA) gene partial sequence homologies of these isolates were identified as Bacillus flexus (BNY11, BWR18), Micrococcus luteus (BWCY16), Photobacterium ganghwense (CSP15), Stenotrophomonas maltophilia (BNO10, BNO20, BSO14), and Vibrio sp. (BNB21). Large and clear zones of inhibition formed by these bacteria against different human pathogenic bacteria were detailed

Preliminary survey of diseases in the coral reefs of Burmanallah coast, Andaman’s

1972-1976<span style="font-size:9.0pt;mso-bidi-font-size:11.0pt; line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:calibri;="" mso-bidi-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">Present study consists the survey conducted <span style="font-size:9.0pt;mso-bidi-font-size:11.0pt; line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:calibri;="" mso-bidi-font-family:"times="" roman";mso-ansi-language:en-in;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="">on the several patterns of diseases in corals from Burmanallah coast of the Andaman Islands. It was observed that Porites sp. were found to be highly sensitive to coral diseases, as they were found with yellow spot disease, pink line syndrome, white line syndrome, white plague and black band diseases. Ring line syndrome was observed only in Acropora humilis and Acropora monticulosa, while abnormal growths were observed in both Acropora humilis and Porites sp. Beside these diseases, bleaching was commonly found in Porites sp. probably due to increase of temperature during the summer season and also may be due to change of salinity attributed to heavy rainfall during both the monsoons. Other factors that were found to cause inhibition of coral growth were sediment deposition, algal mat covers and presence of different bioeroders.</span

CIDMS: Cardiac Integrated Database Management System for Cardiac Systems Biology

A major goal of systems biology is to produce comprehensive models that can predict cellular functions from numerous experimental data such as gene expression profiles, protein-protein interactions (PPI) and profiling of metabolites. It is estimated approximately 20,000 to 27,000 genes are expressed in cardiovascular system 1. The number will be much higher for proteome, as they ar