Localization of Multiple Jellyfish Toxins Shows Specificity for Functionally Distinct Polyps and Nematocyst Types in a Colonial Hydrozoan

Hydractinia symbiolongicarpus is a colonial hydrozoan that displays a division of labor through morphologically distinct and functionally specialized polyp types. As with all cnidarians, their venoms are housed in nematocysts, which are scattered across an individual. Here, we investigate the spatial distribution of a specific protein family, jellyfish toxins, in which multiple paralogs are differentially expressed across the functionally specialized polyps. Jellyfish toxins (JFTs) are known pore-forming toxins in the venoms of medically relevant species such as box jellyfish (class Cubozoa), but their role in other medusozoan venoms is less clear. Utilizing a publicly available single-cell dataset, we confirmed that four distinct H. symbiolongicarpus JFT paralogs are expressed in nematocyst-associated clusters, supporting these as true venom components in H. symbiolongicarpus. In situ hybridization and immunohistochemistry were used to localize the expression of these JFTs across the colony. These expression patterns, in conjunction with known nematocyst type distributions, suggest that two of these JFTs, HsymJFT1c-I and HsymJFT1c-II, are localized to specific types of nematocysts. We further interpret JFT expression patterns in the context of known regions of nematogenesis and differential rates of nematocyst turnover. Overall, we show that JFT expression patterns in H. symbiolongicarpus are consistent with the subfunctionalization of JFT paralogs across a partitioned venom system within the colony, such that each JFT is expressed within a specific set of functionally distinct polyp types and, in some cases, specific nematocyst types

Men’s strategies for preserving emotional well-being in advanced prostate cancer: an interpretative phenomenological analysis

Objective This study explores men with advanced prostate cancers’ own practices for promoting and maintaining emotional well-being using Interpretative Phenomenological Analysis. Design Five men with advanced prostate cancer participated in face-to-face, semi-structured, in-depth interviews. Results Within rich narratives of lost and regained well-being, two super-ordinate themes emerged – ‘living with an imminent and uncertain death’ and ‘holding on to life.’ Well-being was threatened by reduced sense of the future, isolation and uncertainty. Yet, the men pursued well-being by managing their emotions, striving for the future whilst enjoying life in the present, taking care of their families and renegotiating purpose. Running through participant’s accounts was a preference for taking action and problem-solving. Sense of purpose, social connectedness and life-engagement were revealed as concepts central to improving well-being, indicating areas which practitioners could explore with men to help them re-establish personal goals and life-purpose. Conclusions The findings also add weight to the evidence base for the potential value of psychological interventions such as cognitive behaviour therapy and mindfulness in men with prostate cancer

Emergent global oscillations in heterogeneous excitable media: The example of pancreatic beta cells

Using the standard van der Pol-FitzHugh-Nagumo excitable medium model I demonstrate a novel generic mechanism, diversity, that provokes the emergence of global oscillations from individually quiescent elements in heterogeneous excitable media. This mechanism may be operating in the mammalian pancreas, where excitable beta cells, quiescent when isolated, are found to oscillate when coupled despite the absence of a pacemaker region.Comment: See home page http://lec.ugr.es/~julya

A model of ant route navigation driven by scene familiarity

In this paper we propose a model of visually guided route navigation in ants that captures the known properties of real behaviour whilst retaining mechanistic simplicity and thus biological plausibility. For an ant, the coupling of movement and viewing direction means that a familiar view specifies a familiar direction of movement. Since the views experienced along a habitual route will be more familiar, route navigation can be re-cast as a search for familiar views. This search can be performed with a simple scanning routine, a behaviour that ants have been observed to perform. We test this proposed route navigation strategy in simulation, by learning a series of routes through visually cluttered environments consisting of objects that are only distinguishable as silhouettes against the sky. In the first instance we determine view familiarity by exhaustive comparison with the set of views experienced during training. In further experiments we train an artificial neural network to perform familiarity discrimination using the training views. Our results indicate that, not only is the approach successful, but also that the routes that are learnt show many of the characteristics of the routes of desert ants. As such, we believe the model represents the only detailed and complete model of insect route guidance to date. What is more, the model provides a general demonstration that visually guided routes can be produced with parsimonious mechanisms that do not specify when or what to learn, nor separate routes into sequences of waypoints

DASI First Results: A Measurement of the Cosmic Microwave Background Angular Power Spectrum

We present measurements of anisotropy in the Cosmic Microwave Background (CMB) from the first season of observations with the Degree Angular Scale Interferometer (DASI). The instrument was deployed at the South Pole in the austral summer 1999--2000, and made observations throughout the following austral winter. We have measured the angular power spectrum of the CMB in the range 100<l<900 with high signal-to-noise. In this paper we review the formalism used in the analysis, in particular the use of constraint matrices to project out contaminants such as ground and point source signals, and to test for correlations with diffuse foreground templates. We find no evidence of foregrounds other than point sources in the data, and find a maximum likelihood temperature spectral index beta = -0.1 +/- 0.2 (1 sigma), consistent with CMB. We detect a first peak in the power spectrum at l approx 200, in agreement with previous experiments. In addition, we detect a peak in the power spectrum at l approx 550 and power of similar magnitude at l approx 800 which are consistent with the second and third harmonic peaks predicted by adiabatic inflationary cosmological models.Comment: 8 pages, 1 figure, minor changes in response to referee comment

First Intrinsic Anisotropy Observations with the Cosmic Background Imager

We present the first results of observations of the intrinsic anisotropy of the cosmic microwave background radiation with the Cosmic Background Imager from a site at 5080 m altitude in northern Chile. Our observations show a sharp decrease in C_l in the range l=400 - 1500. The broadband amplitudes we have measured are deltaT(band) = 58.7 (-6.3, +7.7) microK for l = 603 (-166, +180) and 29.7 (-4.2, +4.8) microK for l = 1190 (-224, +261), where these are half-power widths in l. Such a decrease in power at high l is one of the fundamental predictions of the standard cosmological model, and these are the first observations which cover a broad enough l range to show this decrease in a single experiment. The C_l we have measured enable us to place limits on the density parameter, Omega(tot) = 0.7 (90% confidence).Comment: 5 pages including 2 figures. Corrected an error in the comparison with Boomerang and Maxim