74 research outputs found
Functional KV10.1 Channels Localize to the Inner Nuclear Membrane
Ectopically expressed human KV10.1 channels are relevant players in tumor biology. However, their function as ion channels at the plasma membrane does not totally explain their crucial role in tumors. Both in native and heterologous systems, it has been observed that a majority of KV10.1 channels remain at intracellular locations. In this study we investigated the localization and possible roles of perinuclear KV10.1. We show that KV10.1 is expressed at the inner nuclear membrane in both human and rat models; it co-purifies with established inner nuclear membrane markers, shows resistance to detergent extraction and restricted mobility, all of them typical features of proteins at the inner nuclear membrane. KV10.1 channels at the inner nuclear membrane are not all transported directly from the ER but rather have been exposed to the extracellular milieu. Patch clamp experiments on nuclei devoid of external nuclear membrane reveal the existence of channel activity compatible with KV10.1. We hypothesize that KV10.1 channels at the nuclear envelope might participate in the homeostasis of nuclear K+, or indirectly interact with heterochromatin, both factors known to affect gene expression
Urine Growth Hormone Determinations Compared with Other Methods in the Assessment of Growth Hormone Secretion
Alkaline phosphatase and ATPases in brush-border membranes of rat jejunum: Distinct effects of divalent cations and of some inhibitors
Short Stature and Low IGF-1 and IGFBP-3 Despite Normal Growth Hormone Secretion in a 4 Year-Old Girl with Primary Empty Sella Syndrome
Clinical Review: Analysis of 3-Hour Growth Hormone Profiles During Sleep in Growth Retarded Children as Diagnostic Criteria of Growth Hormone Neurosecretory Dysfunction
A Novel Polymorphism in Exon 4 of the Growth Hormone Gene and Association of Short Stature with a Known 5' UTR Polymorphism
- …