What, why and how–the policy, purpose and practice of grammatical terminology

© 2018, © 2018 National Association for the Teaching of English. This article critically examines the literature around grammar and grammatical terminology. It is essentially a critical consideration of the debates in England and Wales in four main parts. Part 1 considers debates in policy, the “What”, i.e. grammatical terminology from the perspective of national policy as defined by the English National Curriculum for Key Stages 1 and 2, and the Key Stage 2 “Grammar, Punctuation and Spelling Test”. Part 2, Debates in Purpose, examines the “Why”: it views grammatical terminology through a more theoretical lens which considers the potential purpose and value of explicit grammatical terminology in the classroom. Part 3 touches upon Debates in Practice, the “How”, examining what is already understood about the teaching of grammatical terminology in terms of grammar pedagogy, language acquisition and word learning. While each part has a distinct focus, the field is complex with overlap and interrelated issues. The final part looks briefly at teacher and pupil perspectives

Characterization and screening of IgG binding to the neonatal Fc receptor

The neonatal Fc receptor (FcRn) protects immunoglobulin G (IgG) from degradation and increases the serum half-life of IgG, thereby contributing to a higher concentration of IgG in the serum. Because altered FcRn binding may result in a reduced or prolonged half-life of IgG molecules, it is advisable to characterize Fc receptor binding of therapeutic antibody lead candidates prior to the start of pre-clinical and clinical studies. In this study, we characterized the interactions between FcRn of different species (human, cynomolgus monkey, mouse and rat) and nine IgG molecules from different species and isotypes with common variable heavy (VH) and variable light chain (VL) domains. Binding was analyzed at acidic and neutral pH using surface plasmon resonance (SPR) and biolayer interferometry (BLI). Furthermore, we transferred the well-accepted, but low throughput SPR-based method for FcRn binding characterization to the BLI-based Octet platform to enable a higher sample throughput allowing the characterization of FcRn binding already during early drug discovery phase. We showed that the BLI-based approach is fit-for-purpose and capable of discriminating between IgG molecules with significant differences in FcRn binding affinities. Using this high-throughput approach we investigated FcRn binding of 36 IgG molecules that represented all VH/VL region combinations available in the fully human, recombinant antibody library Ylanthia. Our results clearly showed normal FcRn binding profiles for all samples. Hence, the variations among the framework parts, complementarity-determining region (CDR) 1 and CDR2 of the fragment antigen binding (Fab) domain did not significantly change FcRn binding. 2014 Landes Bioscienc

General Synthetic Strategy to Ordered Mesoporous Carbon Catalysts with Single‐Atom Metal Sites for Electrochemical CO2 Reduction

The electrochemical carbon dioxide reduction reaction (CO2RR) is a transformative technology to reduce the carbon footprint of modern society. Single-site catalysts have been demonstrated as promising catalysts for CO2RR, but general synthetic methods for catalysts with high surface area and tunable single-site metal composition still need to be developed to unambiguously investigate the structure–activity relationship crossing various metal sites. Here, a generalized coordination–condensation strategy is reported to prepare single-atom metal sites on ordered mesoporous carbon (OMC) with high surface areas (average 800 m2 g-1). This method is applicable to a broad range of metal sites (Fe, Co, Ni, Cu, Pt, Pd, Ru, and Rh) with loadings up to 4 wt.%. In particular, the CO2RR to carbon monoxide (CO) Faradaic efficiency (FE) with Ni single-site OMC catalyst reaches 95%. This high FE is maintained even under large current density (>140 mA cm-2) and in a long-term study (14 h), which suits the urgently needed large-scale applications. Theoretical calculations suggest that the enhanced activity on single-atom Ni sites results from balanced binding energies between key intermediates, COOH and CO, for CO2RR, as mediated by the coordination sphere.This is the published version of the following article: Luo, Zhicheng, Zhouyang Yin, Jiaqi Yu, Yu Yan, Bing Hu, Renfeng Nie, Anna F. Kolln et al. "General Synthetic Strategy to Ordered Mesoporous Carbon Catalysts with Single‐Atom Metal Sites for Electrochemical CO2 Reduction." Small 18, no. 16 (2022): 2107799. DOI: 10.1002/smll.202107799. Copyright 2022 The Authors. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0). Posted with permission. DOE Contract Number(s): CBET‐2004808; AC02-07CH11358; SC0018284; AC02-06CH11357; CBET-2004808