Possibilities of alternative generation II biotests at Artemia

The meaning of alternative biotests is described and discussed. The paper also deals with the possible application of the developmental studies of the sea Artemia franciscana nauplinus. Five-day biotests including the validation criteria are described. The possibilities of the biotests are very wide. Additionally to the standard applications in ecotoxicology, there is a possibility of modelling pharmacological experiments or monitoring the effects of ionizing radiation and the interaction with other chemicals

Clinical Symptoms and Histological Changes in Poecilia reticulata following Gamma-Ray Irradiation

Lethality, food intake, clinical symptoms and terminal histological changes were followed after gamma-irradiation with doses of 10, 20, 30, 35 and 40 Gy in guppy, Poecilia reticulata. Clinical symptoms, food intake and longevity were also monitored in the progeny of fish irradiated with a dose of 10 Gy. In the first days after irradiation timidity and lethargy were observed. After doses of 30, 35 and 40 Gy, these symptoms were accompanied with anorexia. The most prominent clinical symptoms observed were emaciation, hampered breathing, exophthalmia and haemorrhages. Histological findings corresponded with these symptoms. In the fish irradiated with 10 or 20 Gy the progeny survived, after a dose of 30 Gy the progeny died within 24 hours after birth and after doses of 35 and 40 Gy dead progeny was born. The survival data provide an estimate of LD50/30 equal to 29 Gy. Higher relative mortality and more severe clinical symptoms were observed in females. The progeny of irradiated parent fish grew and survived for 3 months maximum

Increasing prevalence of overweight and obesity among Tanzanian women of reproductive age intending to conceive: evidence from three Demographic Health Surveys, 2004-2016

# Background The prevalence of people who are overweight or obese is increasing globally, especially in low- and middle-income countries. High body mass index (BMI) among women of reproductive age is a risk factor for various adverse reproductive and pregnancy outcomes. This study aims to describe trends over time in the distribution of BMI among Tanzanian women of reproductive age intending to conceive between 2004/5 and 2015/16, and identify factors associated with high BMI. # Methods We used data on 20,819 women of reproductive age (15-49 years) intending to conceive who participated in the Tanzania Demographic and Health Surveys in 2004/5, 2010 and 2015/16. We estimated the prevalence of high BMI (being overweight \[≥25 to <30 kg/m^2^] and obesity \[≥30kg/m^2^) and trends in the prevalence of high BMI across the three surveys. Using survey-weighted multivariable logistic regression, we used the most recent 2015/16 survey data to identify factors associated with high BMI. # Results Median BMI increased from 21.7kg/m^2^ (inter-quartile range, IQR=19.9-24.1 kg/m^2^) in 2004/5 to 22.0 kg/m^2^ (IQR=20.0-24.8 kg/m^2^) in 2010 to 22.7 kg/m^2^ (IQR=20.4-26.0 kg/m^2^) in 2015/16. The prevalence of overweight women increased from 11.1% in 2004/5 to 15.8% in 2015 (P <0.001). The prevalence of obesity increased from 3.1% in 2004/5 to 8.0% in 2015/16 (P<0.001). Women in the highest wealth quintile had higher odds (adjusted odds ratio, aOR= 4.5; 95%CI 3.4-6.3, P<0.001) of high BMI than women in the lowest quintile. The odds of high BMI were about four times greater (aOR=3.9; 95%CI=2.9-5.4, P<0.001) for women 40-44 years compared to 20–24-year-olds. Women in the high-paying occupations had greater odds of high BMI than those working in agriculture (aOR=1.5; 95% CI=1.1-2.2, P=0.002). Women residing in the Southern zone had 1.9 (95%CI=1.5-2.5, P<0.001) greater odds of high BMI than Lake zone residents. # Conclusions In Tanzania, high BMI affects almost 1 in 4 women of reproductive age who intend to conceive. This contributes to the burden of poor maternal and reproductive health outcomes. We recommend developing and implementing health-system strategies for addressing high BMI, tailored to the modifiable risk factors identified among women of reproductive age

Monitoring of 137CS and 40K in the Levice District, Southern Slovakia

The contamination of the environment, soil and meat of wild animals with radionuclides can negatively affect human health. The aim of our study was to analyse the risk arising from post-Chernobyl contamination of the meat of wild boars (Sus scrofa) originating from the district Levice, southern Slovakia, with the radioactive artificial element 137Cs. The level of natural radionuclide 40K was also determined. We examined altogether 45 samples obtained from 9 wild boars hunted in this area during the period of 2013—2015. From each animal we collected and analysed samples from the thigh, stomach contents, stomach muscles and skin. We also examined samples of soil from the locations where these animals were shot. The activity values of radioactive caesium 137Cs determined in this study were very low and therefore the consumption of wild boar meat originating from this location presents no risk to human health

Radiocaesium in Honey from Košice and Prešov Regions in Eastern Slovakia

The activity of radiocaesium was measured in 50 samples of blossom and honeydew honey obtained in the years 2017 and 2018 from beekeepers from various localities of Košice and Prešov regions, eastern Slovakia. The measurements were carried out by gamma spectrometry in Marinelli-type vessels of volume 450 ml without any previous processing of samples. The activity of radiocaesium in honey samples were low, below the detection level of 0.2 Bq.kg−1 in the majority of samples, and was near the levels measured before the Chernobyl disaster. Higher values were detected in honeydew honey compared to the blossom honey. Consumption of honey from the regions presents no risk of exposure to 137Cs

Dynamics of the double burden of malnutrition in Guatemala: a secondary data analysis of the demographic and health surveys from 1998-2015

Objective: We estimated the prevalence and time trends of the double burden of malnutrition (DBM) in Guatemala and explored its occurrence based on socio-demographic factors. Study design: This was a secondary data analysis using information from four Demographic and Health Surveys covering the period 1998-2015. Methods: The unit of analysis was the household within which information was gathered from women 18-49 years and their children, 6-59 months. The main outcome was the prevalence of any DBM in the household (co-existence of undernutrition and overnutrition in a woman, her children or both). We estimated the prevalence of any DBM by survey and analysed time trends. Stepwise logistic regression was used to explore the occurrence of DBM and socio-demographic factors. Results: We analysed 39,749 households across all surveys. The prevalence of any DBM was 25.3% (95%CI: 22.1-28.7) in 1998-99, 23.8% (22.0-25.8) in 2002, 25.9% (24.3-27.5) in 2008-09 and 24.2% (22.9-25.5) in 2014-15, with no significant change over time (P = 0.782). Characteristics associated with lower odds of any DBM were rural residence, female-headed household, wealth and women's secondary education. Higher odds were seen for households with electricity, women >25y, indigenous and with >2 children. Conclusion: Our findings revealed that a quarter of Guatemala's households suffer from DBM, which has remained unchanged for 17 years. Interventions should prioritise urban areas, households of lower socio-economic status and those less educated. To increase awareness of policymakers of this pressing public health concern, further research on DBM could be strengthened by prospective study designs, integrating all household members and expanding the types of malnutrition.S