EFFECT OF LOCAL RIFAMYCIN APPLICATION ON EXPRESSION OF BMP-2 AND BONE REGENERATION

Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing.Materials and Methods: A standardized 5.0-mm- diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In the Group 1 (n=8 rats) defect was irrigated with rifamycin solution and 25 mg rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the group 2 (n=8 rats) defects were grafted with a gelatin sponge mixed 25 mg rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological slides were prepared from defect site for both immunohistochemical analysis (bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis. Data were analyzed using Mann Whitney U and Kruskall Wallis test.Results: The average new bone formation, number of osteoblast and new vessel formation count were increased more in both of experimental groups in comparison with control group. Anti-BMP-2 labelling (Cell count) was increased more in both of experimental groups in comparison with control group. Conclusion: Local rifamycin application has positive effects on BMP-2 expression and bone regeneration at critical sized bone defects

Yerel Rifamicin Uygulamasının Bmp-2 ve Kemik Rejenerasyon Açısından Etkisi

Amaç: Bu çalışmanın amacı lokal rifamisin uygulamasının kemik iyileşmesi sırasında BMP-2 salınımı üzerine etkisinin değerlendirilmesidir. Materyal ve method: Rat mandibula angulus bölgesinde standart olarak 5 mm çapında kritik boyutta kemik defektleri oluşturulmuştur. Kontrol grubunda (8 rat) defektlere herhangi bir uygulama yapılmamıştır. Birinci deney grubunda (8 rat) defekt bölgesi rifamisin solüsyonu ile irrige edildikten sonra, defekt bölgesine 1, 3 ve 7. günlerde 25 mg rifamisin solüsyonu enjekte edilmiştir. İkinci deney grubunda (8 rat) defekt bölgesi 25 mg rifamisin solüsyonu ile karıştırılmış gelatin sponge ile greftlenmiştir. Cerrahiden 21 gün sonra ratlar sakrifiye edilmiştir. Defekt bölgesinden hem immünhistokimyasal analiz (kemik morfogenetik protein -2 antibody) için hem de histomorfometrik analiz için histolojik kesitler hazırlanmıştır. Elde edilen verilerin analizi Mann Whitney U ve Kruskall Wallis testi kullanılarak yapılmıştır. Bulgular: Deney grubunda kontrol grubuna göre ortalama yeni kemik formasyonu, osteoblast sayısı ve yeni damar oluşum sayısında artış olduğu görülmüştür. Her iki deney grubunda da anti-bmp-2 ile işaretlenmenin (hücre sayma) kontrol grubuna göre daha fazla olduğu görülmüştür. Sonuç: Kritik boyutta kemik defektlerine lokal olarak rifamisin uygulamasının BMP-2 salınımı üzerine pozitif etkileri olduğu tespit edilmiştir

Evaluation of the effects of dual antiplatelet therapy on guided bone regeneration in peri-ımplant bone defect

PMID: 36730057In this study, the authors aim to investigate the effect of dual antiplatelet agents on peri-implant-guided bone regeneraation by studying a sample of rats with titanium implants in their tibias. The rats were randomly divided into 5 groups: acetylsalicylic acid (ASA) (n=10), treated with 20 mg/kg of ASA; ASA+CLPD (Clopidogrel): (n=10), treated with 20 mg/kg of ASA and 30 mg/kg of clopidogrel; ASA+PRSG (Prasugrel): (n=10), treated with 20 mg/kg of ASA and 15 mg/kg of prasugrel; ASA+TCGR (Ticagrelor): (n=10), treated with 20 mg/kg of ASA and 300 mg/kg of ticagrelor; and a control group (n=10) received no further treatment after implant surgery. Bone defects created half of the implant length circumferencial after implant insertion and defects filled with bone grafts. After 8 weeks experimental period, the rats sacrified and implants with surrounding bone tissues were collected to histologic analysis; bone filling ratios of defects (%) and blood samples collected to biochemical analysis (urea, creatinine, aspartate aminotransferase, alanine aminotransferase, phosphorus, magnesium, alkaline phosphatase, calcium, and parathormone). A statistically significant difference was not detected between the groups for all parameters (P>0.05). When the percentage of new bone formation was examined, it was found that there was no statistically significant difference between the groups (P>0.05). Antiplatelet therapy may not adversely affect guided bone regeneration in peri-implant bone defects