Timing is everything - Die Innere Uhr der Pflanzen

Staiger D, Köster T. Timing is everything - Die Innere Uhr der Pflanzen. Labor & More. 2009;5:40-41

Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process

Meyer K, Köster T, Staiger D. Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process. Biomolecules. 2015;5(3):1717-1740.Alternative pre-messenger RNA splicing in higher plants emerges as an important layer of regulation upon exposure to exogenous and endogenous cues. Accordingly, mutants defective in RNA-binding proteins predicted to function in the splicing process show severe phenotypic alterations. Among those are developmental defects, impaired responses to pathogen threat or abiotic stress factors, and misregulation of the circadian timing system. A suite of splicing factors has been identified in the model plant Arabidopsis thaliana. Here we summarize recent insights on how defects in these splicing factors impair plant performance

Funktion von Ribonukleoproteinkomplexen in der posttranskriptionellen Regulation und microRNA-Biogenese

Köster T. Funktion von Ribonukleoproteinkomplexen in der posttranskriptionellen Regulation und microRNA-Biogenese. Bielefeld; 2014

miRNAs - der Hacker-Angriff der Parasiten

Köster T. miRNAs - der Hacker-Angriff der Parasiten. Biologie in unserer Zeit. 2018;48(2):87-88

RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts

Köster T, Staiger D. RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts. Methods in molecular biology (Clifton, N.J.). 2014;1062:679-695.RNA-based regulation is increasingly recognized as an important factor shaping the cellular transcriptome. RNA-binding proteins that interact with cis-regulatory motifs within pre-mRNAs determine the fate of their targets. Understanding posttranscriptional networks controlled by an RNA-binding protein requires the identification of its immediate in vivo targets. Here we describe RNA immunoprecipitation in Arabidopsis thaliana. Transgenic plants expressing an RNA-binding protein fused to green fluorescent protein are treated with formaldehyde to "trap" RNAs in complexes with their physiological protein partners. A whole-cell extract is subjected to immunoprecipitation with an antibody against the GFP tag. In parallel, a mock immunoprecipitation is performed using an unrelated antibody. Coprecipitated RNAs are eluted from the immunoprecipitate and identified via real-time PCR. Enrichment relative to immunoprecipitation from plants expressing GFP only and mock immunoprecipitation with an unrelated antibody indicates specific binding

Plant iCLIP — auf Spurensuche im Transkriptom der Pflanze

Köster T, Sitte A. Plant iCLIP — auf Spurensuche im Transkriptom der Pflanze. BIOspektrum. 2023;29(2):174-176

Plant Ribonomics: Proteins in Search of RNA Partners

Köster T, Meyer K. Plant Ribonomics: Proteins in Search of RNA Partners. Trends in Plant Science. 2018;23(4):352-365

On the move through time - a historical review of plant clock research

Johansson M, Köster T. On the move through time - a historical review of plant clock research. Plant Biology. 2018;21(S1):13-20

On the move through time - a historical review of plant clock research

Johansson M, Köster T. On the move through time - a historical review of plant clock research. Plant Biology. 2018;21(S1):13-20

Unraveling post-transcriptional networks: Analysis of RNA-protein interactions with RNA immunoprecipitation

Meyer K, Köster T, Staiger D. Unraveling post-transcriptional networks: Analysis of RNA-protein interactions with RNA immunoprecipitation. Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology. 2014;95(1):43