The research of clonal relationship among aeromonas strains isolated from human, animal and drinking water by PFGE [PFGE ile i?nsan, hayvan ve i?çme sularından i?zole edilen aeromonas suşları arasında klonal i?lişkinin araştırılması]

Aeromonads infect human through potable water and causes various infections. Their existence in animal are being assessed as potential risk for human health. The aim of this study was to investigate clonal relationship among 52 Aeromonas strains isolated from human with diarrhea (14 strains), healthy food workers (2 strains), animals (24 strains) and drinking water (12 strains) by pulsed-field gel electrophoresis (PFGE). Clonal relation was determined between one diarrheic human isolate and one cattle isolate. No clonal relation was determined between drinking water and human isolates. Two fish isolates, A. caviae and A. sobria, were not distinguished PFGE patterns. Consequently no predominant clone was determined while clonal related strains were determined. Particularly, it is necessary to elicit the epidemiological importance of animals in respect of human Aeromonas infections and extensive studies are required for identification of environmental isolates

Synthesis, structural characterization and biological activity of novel cyclohexane-1,3-dione ligands and their metal complexes

PubMed ID: 26007185Some new Zn(II) and Cu(II) complexes [Cu(L1)(OAc)2]·H2O, [Cu(L1)(NO3)H2O]·NO3·3.5H2O, [Zn(L1)(NO3)2]·4.5H2O, [Zn(L1)(OAc)2(H2O)2]·3H2O, [Cu2(L2)(OAc)4]·2H2O·2DMF, [Cu(L2)2]·2NO3·1.5DMF·H2O, [Zn(L2)2(NO3)2]·DMF and [Zn2(L2)(OAc)4(H2O)4]·5H2O; L1 = 2-[2-(2-methoxyphenyl)hydrazono]cyclohexane-1,3-dione and L2 = 2-[2-(3-nitrophenyl)hydrazono]cyclohexane-1,3-dione were synthesized and characterized by IR, 1H-NMR,13C-NMR and ultraviolet (UV-Vis.) spectroscopy, elemental analysis, magnetic susceptibility, mass spectrometry and thermogravimetrydifferential thermal analysis (TGA-DTA). The synthesized ligands and their complexes were tested for antibacterial activity against Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, and Salmonella typhimurium CCM 583. Some of complexes showed medium-level antibacterial activity against the test bacteria compared with ampicillin. © 2015 by the authors; licensee MDPI, Basel, Switzerland

Synthesis, characterization of poly(E)-3-amino-4-((3-bromophenyl)diazenyl)-1H-pyrazol-5-ol: Investigation of antibacterial activity, fluorescence, and optical properties

The oxidative polycondensation reaction conditions of (E)-3-amino-4-((3-bromophenyl)diazenyl)-1H-pyrazol-5-ol (Schiff base monomer) has been accomplished using NaOCl, and air O 2 oxidants in an aqueous alkaline medium. The optical conductivity, dielectric and optical properties of synthesized poly(Schiff base) were examined. The magnitude of the refractive index decreases with increasing of photon energy up to about 2. 5 eV, then refractive index of the poly(Schiff base) increases with increasing of photon energy. The E 0 and E d values of the poly(Schiff base) were found to be 5.34 and 9.22 eV, respectively. An indirect energy-gap value of the poly(Schiff base) was found to be 0.94±0.009 eV. A direct energy-gap value of the poly(Schiff base) was found to be 1.38±0.01 eV and 1.96±0.009 eV at first region and second region, respectively. Absorption coefficient K changes from 11.87 to 18.25 cm -1. In addition to fluorescence property of poly(Schiff base) was investigated. Finally, poly(Schiff base) and Schiff base monomer were tested for antibacterial activities against some bacteria. © 2012 The Korean Fiber Society and Springer Netherlands

Study of structure and spectral characteristics of the Zinc(II) and Copper(II) complexes with 5,5-dimethyl-2-(2-(3-nitrophenyl) hydrazono)cyclohexane-1,3-dione and their effects on optical properties and the developing of the energy band gap and investigation of antibacterial activity

A new chelating ligand, 5,5-dimethyl-2-(2-(3-nitrophenyl) hydrazono)cyclohexane-1,3-dione (L), and four transition metal complexes were synthesized. The ligand and its complexes were characterized by IR, 1H and 13C-NMR spectra, elemental analyses, magnetic susceptibility, mass spectra, UV-Vis. and thermogravimetry-differential thermal analysis (TGA-DTA). The metal complexes showed different geometric structures. Antibacterial tests of the ligand and complexes were also done. The Zn(II) complexes showed antibacterial activity against Staphylococcus aureus ATCC-25923 at middle level. The optical properties of the ligand and its complexes were extensively investigated by optical measurements. The average transmittance value of the ligand was the highest value, while the average transmittance value of the [CuL2(NO3)2]·6H2O complex was the lowest value. The optical band gap of the ligand, [(Zn)2L(OH)4]·2DMF, [ZnL(NO3)2(H2O)2]·5H2O, [CuL2(OAc)]·OAc·3.5H2O and [CuL2(NO3)2]·6H2O complexes were calculated and 3.121 eV, 2.180 eV, 1.551 eV, 2.489 eV and 1.556 eV values were found, respectively. The complexes decreased the optical band gap of the ligand. The optical band gap value of the [ZnL(NO3)2(H2O)2]·5H2O complex is the lowest value. The optical band gap value of the ligand is the highest value. The complexes decrease the angle of incidence of the ligand, while complexes increase the angle of refraction of the ligand. The electric susceptibility of the ligand decreases with its complexes. © 2014, Sociedad Química de México

Prevalence of Cryptosporidium spp. in asymptomatic food workers.

Cryptosporidium spp. is obligatory intracellular parasite and causes intestinal infection. In intestine infections in the form of sporadic and epidemics, food and accordingly workers in food sector may play a role as the source of infection. In this study, it is aimed to reveal the existence of asymptomatic cryptosporidiosis. In the study, stool samples of 393 workers -employed at various branches of food sector in the region of Van- are used. In order to detect Cryptosporidium spp. oocysts, Modified Ziehl Neelsen (MZN) Staining was used. In this study, asymptomatic cryptosporidiosis has been detected in 5 (1.27%) of 393 workers. Epidemiological findings indicate that food workers can be source of cryptosporidiosis outbreak. Thus, searching for the existence of asymptomatic cryptosporidiosis food workers -which epidemiologically has potential significance- and taking the required measures in case of its determination are significant in respect of public health