1 research outputs found
Biofilm formation of Candida Spp. isolated from the vagina and antibiofilm activities of lactic acid bacteria on the these Candida Isolates
Background: In this study, it was aimed to investigate the effects of
bacterial cells and cell-free filtrates of Lactobacillus acidophilus
8MR7 and Lactobacillus paracasei subspecies paracasei 10MR8 on the
biofilm formation of 3 Candida tropicalis , 3 C. glabrata and 12 C.
albicans isolated from the vagina and identified their virulence
factors. Methods: Haemolytic activities esterase activities, and
phospholipase activities as virulence factors of Candida strains were
determined. Biofilm formations of these isolates were determined by
Congo Red agar and microtitration plate method. Antibiofilm activities
of bacterial cells and cell-free filtrates of L. acidophilus 8MR7 and
L. paracasei subspecies paracasei 10MR8 on Candida isolates were
determined by the microtitration plate method. Results: Bacterial cells
of L. acidophilus 8MR7 and L. paracasei subspecies paracasei 10MR8 were
not very effective in the inhibition of biofilm, whereas it has been
observed that the cell-free filtrates of these bacteria inhibit the
formation of biofilms of Candida strains. Although the main mechanism
for inhibiting the formation of Candida spp. biofilm is the competition
for adhesion, it is concluded that the substances contained in the
cell-free filtrates of lactic acid bacteria are also important.
Conclusion: These isolates promise hope as potential bacteria that can
be used for anti-adhesion purposes in health-care materials