PRELIMINARY PHTOCEMICAL INVESTIGATION AND ANTI-VENOM ACTIVITY OF COIX LACRYMAJOBI ROOT EXTRACT AGAINST DABOIA RUSSELLI VENOM-INDUCED MYONECROSIS

Objective: The aim of the study was to carry out the preliminary phytochemical investigation and to evaluate the inhibition of Daboia russellivenom‑induced myonecrosis by root extract (RE) of Coix lacrymajobi.Methods: The roots of C. lacrymajobi were subjected to differential extraction by soxhlet extraction using petroleum ether, chloroform, ethyl acetate,and ethanol. The resultant extracts were subjected to the preliminary phytochemical investigation to identify the different chemical groups present inthe extracts. Myonecrotic activity was conducted, to assess the ability of ethanolic RE to inhibit the myonecrosis induced by D. russelli venom in rats.Results: The preliminary phytochemical investigation revealed the presence of triterpenoids, resins, steroids and fixed oils in petroleum ether extract,flavonoids, triterpenoids, saponins and fixed oils in chloroform extract and alkaloids, carbohydrates, flavonoids, glycosides, resins, saponins, steroids,and tannins. Ethanolic extract was found to have maximum number of phytochemicals, and hence, it was used for further study. The ethanolic REsignificantly inhibited the myonecrotic activity at dose level 200 and 400 mg/kg body weight.Conclusion: The screening of phytochemicals presents on the different fractions of the RE was studied successfully. Supporting the use of roots bytraditional healers, ethanolic extract successfully inhibited D. russelli venom‑induced myonecrosis in rats.Keywords: Coix lacrymajobi, Daboia russelli, Myonecrosis

INHIBITORY EFFECT OF ETHANOLIC ROOT EXTRACT OF COIX LACHRYMAJOBI ON HYALURONIDASE AND L-AMINO ACID OXIDASE OF NAJA NAJA AND DABOIA RUSSELLI VENOM

Objective: This study was carried out to investigate the efficacy of inhibitory effect of ethanolic root extract (ERE) of plant Coix lachrymajobi (Poaceae) on hyaluronidase and L-amino acid oxidase (LAAO) of Daboia russelii and Naja naja venom. Methodology: The ethanolic root extract of C. lachrymajobi is used to treat snake bite victims by traditional healers as folk medicine for centuries, was tested in-vitro to determine its ability to inhibit D. russelli and N. naja hyaluronidase and LAAO activities.  In-vitro studies were carried out with different doses of ERE.Results & Discussion: which effectively neutralized hyaluronidase and LAAO activities of both D. russelli and N. naja venom.  ERE at doses of 2000µg successfully inhibited D. russelli (50 µg) hyaluronidase activity by 67.04% and LAAO activity by 77.86%.  The hyaluronidase activity of N. naja venom (50 µg) was inhibited by 71.83% and LAAO activity was reduced by 74.83%.  From the results, It is evident that the ERE of Coix lachrymajobi effectively neutralizes important toxic enzymes of the D. russelli and Naja naja venoms.Keywords: Daboia russelii; Naja naja; Coix lacryma-jobi; Hyaluronidase; L-amino acid oxidas