Objective: The aim of the study was to carry out the preliminary phytochemical investigation and to evaluate the inhibition of Daboia russellivenom‑induced myonecrosis by root extract (RE) of Coix lacrymajobi.Methods: The roots of C. lacrymajobi were subjected to differential extraction by soxhlet extraction using petroleum ether, chloroform, ethyl acetate,and ethanol. The resultant extracts were subjected to the preliminary phytochemical investigation to identify the different chemical groups present inthe extracts. Myonecrotic activity was conducted, to assess the ability of ethanolic RE to inhibit the myonecrosis induced by D. russelli venom in rats.Results: The preliminary phytochemical investigation revealed the presence of triterpenoids, resins, steroids and fixed oils in petroleum ether extract,flavonoids, triterpenoids, saponins and fixed oils in chloroform extract and alkaloids, carbohydrates, flavonoids, glycosides, resins, saponins, steroids,and tannins. Ethanolic extract was found to have maximum number of phytochemicals, and hence, it was used for further study. The ethanolic REsignificantly inhibited the myonecrotic activity at dose level 200 and 400 mg/kg body weight.Conclusion: The screening of phytochemicals presents on the different fractions of the RE was studied successfully. Supporting the use of roots bytraditional healers, ethanolic extract successfully inhibited D. russelli venom‑induced myonecrosis in rats.Keywords: Coix lacrymajobi, Daboia russelli, Myonecrosis
