2 research outputs found
Computational studies on new Leishmanial drug targets against Quercetin
Leishmaniasis, a parasitic disease caused by Leishmania parasite which resides in the infected sand flies. Control of Leishmaniasis remains a source of grave concern worldwide. Studies on Leishmaniasis triggered because of its outbreak in tropical and subtropical regions of Asia, East Africa and South America. There is an urgent need for new therapeutic interventions such as vaccine and new drug targets as it develops resistance towards the available drugs. Quercetin, a derivative of polyphenolic flavonoid exhibits various biological activities by interacting with proteins and nucleic acids. In this study, computational analysis was performed to identify the potential drug target of Quercetin in Leishmania species by molecular docking. The newly predicted targets were subjected for subcellular localization prediction and determined the protein-protein interaction networks that would aid in the development of anti-Leishmanial drugs. This study helps in the identification of targets and development of anti-Leishmanial drugs
<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Effect of acute exposure of N, N-Dimethylformamide, an industrial solvent on lipid peroxidation and antioxidants in liver and kidney of rats</span>
279-284<span style="mso-bidi-font-weight:
bold" lang="EN-GB">N, N-Dimethylformamide (DMF), an industrial
solvent widely used throughout the world is a known toxic compound. Here, we
studied the effects of acute exposure of DMF on liver and kidney in rats. Rats
were treated intraperitoneally with a
single dose of DMF (1.5 g/kg) for 24 and 48 h. Hepatic and nephrotoxicity was
confirmed based on the significant increase in the serum levels of aspartate
amino transferase, alanine amino transferase, alkaline phosphatase, g-glutamyl transferase, urea,
creatinine and electrolytes. Oxidative stress was assessed by determining the
levels of lipid peroxidation (LPO) and antioxidants in liver and kidney.
The LPO levels were elevated in both the tissues upon DMF exposure, whereas the
activities of enzymatic antioxidants SOD, CAT and Gpx and non-enzymatic
antioxidants (glutathione and vitamin C)
were declined. The hepatic- and nephrotoxicity was further confirmed by the increasing
incidence of inflammation in the histopathological studies. The findings
indicate that acute exposure of DMF results in oxidative stress, antioxidant
deficiency, attenuating liver and kidney marker enzymes, resulting in tissue
inflammation and damage.
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