Persuading the enemy: estimating the persuasive effects of partisan media with the preference-incorporating choice and assignment design

Does media choice cause polarization, or merely reflect it? We investigate a critical aspect of this puzzle: how partisan media contribute to attitude polarization among different groups of media consumers. We implement a new experimental design, called the Preference-Incorporating Choice and Assignment (PICA) design, that incorporates both free choice and forced exposure. We estimate jointly the degree of polarization caused by selective exposure and the persuasive effect of partisan media. Our design also enables us to conduct sensitivity analyses accounting for discrepancies between stated preferences and actual choice, a potential source of bias ignored in previous studies using similar designs. We find that partisan media can polarize both its regular consumers and inadvertent audiences who would otherwise not consume it, but ideologically-opposing media potentially also can ameliorate existing polarization between consumers. Taken together, these results deepen our understanding of when and how media polarize individuals.Accepted manuscrip

Parallel Genome-Wide Expression Profiling of Host and Pathogen During Soybean Cyst Nematode Infection of Soybean

Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential expression across different stages of nematode parasitism and development. Differentially expressed SCN genes were grouped into nine different clusters based on their expression profiles during parasitism of soybean roots. The patterns of gene expression we observed in SCN suggest coordinated regulation of genes involved in parasitism. Quantitative real-time reverse-transcription polymerase chain reaction confirmed the results of our microarray analysis. The simultaneous genome-wide analysis of gene expression changes in the host and pathogen during a compatible interaction provides new insights into soybean responses to nematode infection and the first profile of transcript abundance changes occurring in the nematode as it infects and establishes a permanent feeding site within a host plant root

Sequence mining and transcript profiling to explore cyst nematode parasitism

<p>Abstract</p> <p>Background</p> <p>Cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins. These proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants.</p> <p>Results</p> <p>We present here the expression patterns of all previously described parasitism genes of the soybean cyst nematode, <it>Heterodera glycines</it>, in all major life stages except the adult male. These insights were gained by analyzing our gene expression dataset from experiments using the Affymetrix Soybean Genome Array GeneChip, which contains probeset sequences for 6,860 genes derived from preparasitic and parasitic <it>H. glycines </it>life stages. Targeting the identification of additional <it>H. glycines </it>parasitism-associated genes, we isolated 633 genes encoding secretory proteins using algorithms to predict secretory signal peptides. Furthermore, because some of the known <it>H. glycines </it>parasitism proteins have strongest similarity to proteins of plants and microbes, we searched for predicted protein sequences that showed their highest similarities to plant or microbial proteins and identified 156 <it>H. glycines </it>genes, some of which also contained a signal peptide. Analyses of the expression profiles of these genes allowed the formulation of hypotheses about potential roles in parasitism. This is the first study combining sequence analyses of a substantial EST dataset with microarray expression data of all major life stages (except adult males) for the identification and characterization of putative parasitism-associated proteins in any parasitic nematode.</p> <p>Conclusion</p> <p>We have established an expression atlas for all known <it>H. glycines </it>parasitism genes. Furthermore, in an effort to identify additional <it>H. glycines </it>genes with putative functions in parasitism, we have reduced the currently known 6,860 <it>H. glycines </it>genes to a pool of 788 most promising candidate genes (including known parasitism genes) and documented their expression profiles. Using our approach to pre-select genes likely involved in parasitism now allows detailed functional analyses in a manner not feasible for larger numbers of genes. The generation of the candidate pool described here is an important enabling advance because it will significantly facilitate the unraveling of fascinating plant-animal interactions and deliver knowledge that can be transferred to other pathogen-host systems. Ultimately, the exploration of true parasitism genes verified from the gene pool delineated here will identify weaknesses in the nematode life cycle that can be exploited by novel anti-nematode efforts.</p

Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. Within the first 12 h after inoculation (hai), which corresponds to fungal germination and penetration of the epidermal cells, differential gene expression changes were evident in both genotypes. mRNA expression of these genes mostly returned to levels found in mock-inoculated plants by 24 hai. In the susceptible genotype, gene expression remained unaffected by rust infection until 96 hai, a time period when rapid fungal growth began. In contrast, gene expression in the resistant genotype diverged from the mock-inoculated control earlier, at 72 h, demonstrating that Rpp2-mediated defenses were initiated prior to this time. These data suggest that ASR initially induces a non-specific response that is transient or is suppressed when early steps in colonization are completed in both soybean genotypes. The race-specific resistance phenotype of Rpp2 is manifested in massive gene expression changes after the initial response prior to the onset of rapid fungal growth that occurs in the susceptible genotype

Spin-resolved electron-impact ionization of lithium

Electron-impact ionization of lithium is studied using the convergent close-coupling (CCC) method at 25.4 and 54.4 eV. Particular attention is paid to the spin-dependence of the ionization cross sections. Convergence is found to be more rapid for the spin asymmetries, which are in good agreement with experiment, than for the underlying cross sections. Comparison with the recent measured and DS3C-calculated data of Streun et al (1999) is most intriguing. Excellent agreement is found with the measured and calculated spin asymmetries, yet the discrepancy between the CCC and DS3C cross sections is very large

A Detailed Study of the Lobes of Eleven Powerful Radio Galaxies

Radio lobes of a sample of eleven very powerful classical double radio galaxies were studied. Each source was rotated so that the symmetry axis of the source was horizontal, and vertical cross-sectional cuts were taken across the source at intervals of one beam size. These were used to study the cross-sectional surface brightness profiles, the width of each slice, radio emissivity as a function of position across each slice, the first and second moments, and the average surface brightness, minimum energy magnetic field strength, and pressure of each slice. A Gaussian provides a good description of the surface brightness profile of cross-sectional slices. The Gaussian FWHM as a function of distance from the hot spot first increases and then decreases with distance from the hot spot. The width as a function of distance from the hot spot is highly symmetric on each side of the source. The radio emissivity is often close to flat across a slice, indicating a roughly constant emissivity and pressure for that slice. Some slices show variations in radio emissivity that indicate an ``edge-peaked'' pressure profile for that slice; these often occur in slices near the local maxima of the bridge width. The emissivity does not exhibit any signature of emission from a jet. The first moment is generally quite close to zero indicating only small excursions of the ridge line from the symmetry axis of the source. The second moment indicates the same source shape as is found using the Gaussian FWHM. The average magnetic field strength and pressure decrease with increasing distance from the hot spot, reaching a roughly constant value at a location that is typically just before the location of a local maximum of the bridge width. These results are interpreted in terms of a heuristic model for the radio lobes.Comment: 102 pages, 136 figures, accepted for publication in ApJ Supplement Serie

Divergent evolution of arrested development in the dauer stage of Caenorhabditis elegans and the infective stage of Heterodera glycines

The generation and analysis of over 20,000 ESTs allowed the identification and expression profiling of 6,860 predicted genes in the nematode Heterodera glycines. This revealed that gene expression patterns in the dauer stage of Caenorhabditis elegans are not conserved in H. glycines

Gauge symmetry and the EMC spin effect

We emphasise the EMC spin effect as a problem of symmetry and discuss the renormalisation of the $C=+1$ axial tensor operators. This involves the generalisation of the Adler-Bell-Jackiw anomaly to each of these operators. We find that the contribution of the axial anomaly to the spin dependent structure function $g_1 (x, Q^2)$ scales at $O(\alpha_s)$. This means that the anomaly can be a large $x$ effect in $g_1$. Finally we discuss the jet signature of the anomaly.Comment: 17 pages, Latex, Cavendish preprint HEP 93/