1 research outputs found
Ratiometric Imaging of Tissue by Two-Photon Microscopy: Observation of a High Level of Formaldehyde around Mouse Intestinal Crypts
Ratiometric
imaging by two-photon microscopy can offer a viable
tool for the relative quantification of biological analytes inside
tissue with minimal influence from environmental factors that affect
fluorescence signal. We demonstrate the ratiometric imaging of formaldehyde
at the suborgan level using a two-photon fluorescent probe, which
involves pixel-to-pixel ratiometric data transformation. This study
reveals for the first time a high level of formaldehyde around the
crypts of mouse small intestine, implicating its possible protective
role along with the released antimicrobials from the Paneth cells