Strains, plasmids, primers.

<p>Str  =  streptinomycin; Tc  =  tetracycline; Sp  =  spectinomycin; Gm  =  gentamicin; <i>Bam</i>HI sites in primers are underlined.</p

Symbiotic properties of 4 week old bean plants.

<p>The values are the mean ± SEM of plants inoculated with RU2222 (n = 8) and RU1933 (n = 6). P-values were determined by ttest.</p

Plant dry weights and acetylene reduction (AR) of alfalfa (<i>M. sativa</i>) at 4 weeks.

<p>The values are the mean ± SEM of plants inoculated with 2011 (n = 18), LMB211 (n = 18) and uninoculated control plants (n = 6). ND  =  not determined.</p

Transport of amino acids and plant dry weights by strains of <i>R. leguminosarum</i> bv <i>phaseoli.</i>

<p>All strains were grown on AMS glucose/NH₄Cl minimal medium. Rates of uptake are expressed as nmol min⁻¹mg⁻¹ protein. Values are the mean ± SEM of determinations from three or more independent cultures. Dry weights (g plant⁻¹) are averages of >27 plants.</p

TEM pictures of bacteroids of wild-type (A and B) and RU1933 <i>aap bra</i> (C and D).

<p>A and C are pictures taken of 4 week old nodules, B and D of 6 week old nodules. Size bars are 500 µm.</p

Transport of amino acids by strains of <i>R. leguminosarum</i> bv. <i>viciae.</i>

<p>Vmax and Km were calculated from transport rate mean values of 3 independent cultures measured at 5 concentrations from 0.1 µM up to 5 µM. Vmax values are given in nmol min⁻¹mg⁻¹ protein ± SEM and Km mean values are given in nM ± SEM. ND  =  not determined.</p

Transport of amino acids by strains of <i>Sinorhizobium meliloti.</i>

<p>All strains were grown in M9 modified glucose/NH₄Cl minimal medium. Rates of uptake are expressed as nmol min⁻¹mg⁻¹ protein. Values are the mean ± SEM of determinations from three independent cultures, except for strain 2011 alanine, leucine and valine transports, which just served as controls and represent single measurements. ND  =  not determined.</p

Transport rates of branched-chain amino acids and plant dry weights by strains of <i>R. leguminosarum</i> bv. <i>viciae.</i>

<p>Transport rates are the mean ± SEM of 3 independent cultures expressed as nmol min⁻¹mg⁻¹ protein. Dry weights (g plant⁻¹) are averages of 15 plants.</p

Symbiotic properties of 5 and 6 week old bean plants.

<p>The rates of acetylene reduction and numbers of nodules by plants inoculated with RU1933 were significantly different from those of plants inoculated with wild-type RU2222 (p = 0.01; n≥6). ND  =  not determined. P-values were determined by ttest.</p

Growth of beans infected by different strains of <i>R. leguminosarum</i> bv. <i>phaseoli</i>.

<p>Plants were inoculated with RU2222 (wild-type), RU1933 (<i>aapJQM</i>::ΩSp <i>braE</i>::Tn<i>phoA</i>) or left uninoculated.</p