Immunofluorescence analysis of CD44 and E-selectin expressions after injury.

<p>(A) (B)GFP positive cells (green) colocalized with CD44 and E-selectin (red). Nuclear staining with DAPI is blue. (C) (D)Immunofluorescence analysis of CD44 and E-selectin in the wound sites. The CD44 and E-selectin signal are stronger in the wild-type mice. Scale bars indicate 50 μm. * <i>p</i> < 0.01, (n = 5), determined by Student's t-test.</p

Immunofluorescence analysis of CD31, vWF and keratin 14 expressions in wound sites.

<p>(A) GFP positive cells (green) were colocalized with CD31 (red), (B)vWF (red) and (C)keratin 14. Nuclear staining with DAPI is blue. (D)(E)(F) Immunofluorescence analysis of CD31, vWF and keratin14 in wound sites. These data suggest that MSCs differentiated into endothelial cells and keratinocytes, respectively. Scale bars indicate 50 μm. * <i>p</i> < 0.01, (n = 5), determined by Student's t-test.</p

Skin wound healing analysis in wild-type and <i>Opn</i>^<i>-/-</i> mice.

<p>(A) A schematic diagram illustrating the location and dimensions of the full-thickness excisional and incisional wounds made to the shaved dorsal skin of adult male mice. The dotted lines indicate the axes of sections. Blue circles indicate MSCs injection and red represents PBS control injection. (B) Observation of wild-type and <i>Opn</i>^<i>-/-</i> wounds at various time points after wounding. (C) The proportion of wound healing relative to the initial wound area at each time point after injury in the control (open bars) versus <i>Opn</i>^<i>-/-</i> wounds. * <i>p</i> < 0.05 and **<i>p</i> < 0.01, (n = 6), determined by a one-way ANOVA.</p

The effects of rOPN on the migration of MSCs.

<p>(A) MSCs were seeded on transwells with the presence of 25nM rOPN on the bottom. (B) After 24 hours, the number of cells present on the bottom side of the transwells’ filter was counted and analyzed. Scale bars indicate 50 μm. *and **<i>p</i> < 0.01(n = 4), determined by a one-way ANOVA.</p