The effects of cumulus cells on the fertility and developmental ability of C57BL/6J mouse oocytes after IVF.

<p>Data are means±S.E.M. ^a-b,c-d,e-f Different superscripts denote a significant difference (<i>P</i><0.05). In each treatment group, more than 100 oocytes were examined. PN, pronuclear stage; COC, cumulus oocyte complexes; DO, denuded oocytes.</p

The effects of cumulus cells on the fertility and developmental ability of vitrified C57BL/6J mouse oocytes after IVF.

<p>IVF was carried out using frozen-thawed BDF1 mouse sperm. Data are means±S.E.M. ^a-b,c-d,e-f Different superscripts denote significant difference (<i>P</i><0.05). In each treatment group, more than 100 oocytes were examined.</p

In vivo development of vitrified COCs from C57BL/6J mouse after in vitro fertilization.

<p>In vivo development of vitrified COCs from C57BL/6J mouse after in vitro fertilization.</p

Generation of offspring after ICSI using sperm from cryopreserved porcine testicular xenografts.

<p>Two male and 3 female piglets were born after transfer of fertilized oocytes produced by ICSI using sperm recovered from a mouse on day 254 in the 20-min group. Tissue was stored in LN₂ for 188 days. Photographed on the fifth day after birth.</p

Histological appearance of cryopreserved porcine testicular tissues before and after xenografting, and of recovered sperm.

<p>(A) Fresh testicular tissue from a 9-day-old piglet before rinsing with base solution. Tissues were vitrified after being immersed in vitrification solution for 10 or 20 min (10- or 20-min group), stored in LN₂ and then warmed: tissue in the (B) 10- and (C) 20-min group contained distinct gonocytes (arrows). Tissue recovered from mice in the (D) 10-min and (E) 20-min groups on day 230−350 contained elongated spermatids and sperm (arrow heads). Bars  = 50 μm. (E) Sperm retrieved from xenografts on days 230−350. Bar  = 10 μm.</p

Weights of cryopreserved porcine testicular tissue in mice.

<p>All visible grafts were recovered from the recipient mice, and values are means of total weights per mouse (± SEM) for 4 to 7 mice in each group.</p

The effects of COOH-PLL on survival, fertility and developmental ability of vitrified mouse oocytes after IVF.

<p>Data are shown as means ± S.E.M. Different superscripts denote a significant difference (P<0.05). Numbers of oocytes used in each group were described under the treatment group.</p

Hormonal profiles of the recipient mice.

<p>Serum concentrations of (A) inhibin and (B) testosterone in the recipient mice after castration and after grafting of cryopreserved porcine tissue of the 10- and 20-min groups are shown. Values are means ± SEM for 4 to 7 mice.</p

The effect of COOH-PLL concentration on the number of cells in a blastocyst.

<p>The effect of COOH-PLL concentration on the number of cells in a blastocyst.</p

The permeability of PN mouse and pig embryos to COOH-PLL.

<p>The embryos were exposed to FITC-labeled COOH-PLL (5% (w/v)) for 5 min. After exposure, embryos with or without washing were examined under a laser scanning microscope. Scale bars denote 50 um.</p