Decreased Leptin Is Associated with Alterations in Thyroid-Stimulating Hormone Levels after Roux-en-Y Gastric Bypass Surgery in Obese Euthyroid Patients with Type 2 Diabetes

Background: Leptin has been shown to stimulate the hypothalamus-pituitary-thyroid (HPT) axis in vivo and vitro. Its role in thyroid axis regulation after weight loss induced by bariatric surgery is still unknown. The aim of this study was to evaluate the influence of leptin on weight loss and thyroid function variation induced by Roux-en-Y gastric bypass (RYGB) surgery in euthyroid individuals with obesity and type 2 diabetes mellitus (T2DM). Methods: 65 Chinese individuals with obesity and T2DM who underwent RYGB, and 27 healthy volunteers were enrolled in this retrospective study. Participants were evaluated for changes in anthropometric parameters, metabolic indexes, thyroid function, and leptin levels before and 12 months after surgery. Results: After RYGB, all of these patients experienced significant weight reduction and improved glucose control. Metabolic parameters were significantly ameliorated after surgery compared with baseline. Thyroid hormones including free triiodothyronine (FT3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH) declined in parallel. Median (IQR) plasma leptin levels decreased from 33.7 ng/mL (17.9–63.1) to 10.3 ng/mL (4.0–18.5). Pearson correlation analysis showed that TSH was significantly positively correlated with body mass index, C-reactive protein (CRP), and leptin. Multiple stepwise linear regression indicated that leptin and CRP were independent factors affecting TSH. The β coefficients were 0.38 (p = 0.001) and 0.32 (p = 0.004), respectively. There was a significant positive correlation between ΔTSH and Δleptin (r = 0.33, p = 0.01). Conclusion: Decreased or normalized TSH levels after weight loss induced by RYGB might be mediated by the decline in leptin. There could be cross talk between adipose tissue and the HPT axis

Converting Layered Zinc Acetate Nanobelts to One-dimensional Structured ZnO Nanoparticle Aggregates and their Photocatalytic Activity

We were successful in synthesizing periodic layered zinc acetate nanobelts through a hydrothermal solution process. One-dimensional structured ZnO nanoparticle aggregate was obtained by simple thermal annealing of the above-mentioned layered ZnO acetate nanobelts at 300 °C. The morphology, microstructure, and composition of the synthesized ZnO and its precursors were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and infrared spectroscopy, respectively. Low angle X-ray diffraction spectra reveal that as-synthesized zinc acetate has a layered structure with two interlayer d-spacings (one is 1.32 nm and the other is 1.91 nm). SEM and TEM indicate that nanobelt precursors were 100–200 nm in width and possesses length up to 30 μm. Calcination of precursor in air results in the formation of one-dimensional structured ZnO nanoparticle aggregates. In addition, the as-prepared ZnO nanoparticle aggregates exhibit high photocatalytic activity for the photocatalytic degradation of methyl orange (MO)

Uncovering the effects of policies, climate, and economic development on carbon neutrality in southern Tibet, China

Partitioning the contributions of climate, economic growth, and policy to a region’s carbon flows is an important process for the Chinese government seeking to optimize their regional development policies to achieve carbon neutrality by 2060. A combination of the carbon emission analysis and human appropriation of net primary production (HANPP) framework was applied to a village in the Lhasa river valley, Tibet, to quantify the contributions of these different factors to carbon flows and neutrality. From 2010 to 2019, the average annual net sequestration of CO2 was 374.9 g CO2 m−2 a−1. Changes in climate conditions and the regional policy of Grassland Ecological Protection Subsidy and Reward increased carbon sequestrations by 409.5 and 25.7 g CO2 m−2 a−1, respectively. Socioeconomic development, policies for reducing poverty, and promotion of forage production led to the increase in CO2 emissions by 103.5, 88.8, and 4.3 g CO2 m−2 a−1, respectively. The cumulative CO2 emissions (including HANPP) caused by land use were 298.92 Tg CO2 (2479.63 g CO2 m−2; 1 Tg = 1012 g), while the cumulative CO2 emissions due to energy use were only 11.22 Tg CO2 (93.07 g CO2 m−2), equal to 3.75% of the CO2 emissions driven by land use. Livestock grazing and cropland cultivation were the two main land use factors affecting the carbon balance. We argue that unhooking economic growth from traditional nomadic animal husbandry and lifestyles through policy optimizations would highly contribute the carbon neutrality in Tibet

COM33 suppresses carboplatin-induced epithelial-mesenchymal transition via inhibition of Twist1 in ovarian cancer

Despite favorable responses to platinum-based chemotherapy in ovarian cancer (OC), chemoresistance is still a major cause of treatment failure. Hence, we develop a novel synthetic agent, COM33, to relieve the chemoresistance caused by carboplatin. The anti-cancerous effects of the combination of COM33 and carboplatin on OC are evaluated by cell viability, wound healing, and transwell invasion assays. A mechanistic investigation is carried out by using RNA-Seq analysis and then verified by western blot analysis and immunofluorescence microscopy. The safety and efficacy in vivo are evaluated using SKOV3 tumor-bearing nude mice. Results show that the co-administration of COM33 enhances the inhibitory effects of carboplatin on cancer cell viability, migration, and invasion in vitro and tumor growth in vivo. Furthermore, COM33 suppresses the carboplatin-induced epithelial-mesenchymal transition (EMT) by inhibiting the ERK signaling pathway. Additionally, we show that Twist1, the effector of the ERK signaling pathway, participates in carboplatin-induced EMT and is also inhibited by COM33. Our data show that the combination of carboplatin with COM33 is beneficial for chemotherapy against OC, which may be a potential novel anti-tumor strategy

Study on Preparation and Characterization of Graphene Based on Ball Milling Method

Taking advantage of the state-of-the-art graphene preparation technologies in China and abroad, this work studied the preparation processes of graphene for road applications based on the preliminary high-speed vibration ball milling method. This work combined numerical modeling and microscopic experiments. The preparation parameters were optimized through a multifactor and multilevel test scheme. The materials, influencing factors, and parameters in the preparation process were systematically studied. A graphene preparation method was proposed that considered vibration frequency, static filling rate, material-to-ball volume ratio, and the void percentages of media. Flake graphite, aluminum powder, and 304 stainless-steel grit were used as the preparation materials. The preparation parameters and process model were established based on the uniform design method. The preparation parameters were proposed, calculated, and optimized. Microscopic analysis showed that the proposed preparation method can improve the quality of graphene. This study provides a new source of raw materials for the application of graphene in road engineering

Density Management Is More Cost Effective than Fertilization for <i>Chimonobambusa pachystachys</i> Bamboo-Shoot Yield and Economic Benefits

Stand-density management and fertilization practices are the main two factors affecting bamboo-shoot yield. However, the appropriate density and fertilization rates are still unclear for improving the bamboo-shoot yield and its economic benefits, especially for a high economic value bamboo-shoot forest. To fill this gap, we conducted a two-year split-plot design experiment in a Chimonobambusa pachystachys shoot forest. The main plots were assigned to five density rates, 40,000, 50,000, 60,000, 70,000, and 100,000 culms ha−1, and the subplots were assigned to four fertilization rates (nitrogen:phosphorus:potassium = 23:3:15): 0, 820, 1640, and 2460 kg ha−1 a−1. Results showed that the bamboo-shoot yield increased first and then decreased with stand density, while it increased with fertilization rates. Density management and fertilization regulate bamboo-shoot yield by changing the soil’s Olsen P, available nitrogen, organic matter, and available potassium contents. The maximum bamboo-shoot yield was 9315.92 kg ha−1, which appeared in the density of 60,000 culms ha−1 and the fertilization of 2460 kg ha−1 a−1. However, the maximum bamboo-shoot net profit was 135,242.63 CNY ha−1, which appeared at the density of 60,000 culms ha−1 and the fertilization of 1640 kg ha−1 a−1. The economic-benefit analysis shows that density management achieves a net-profit growth comparable to fertilizer application at a much lower cost. The study results provide a basis for the scientific management of C. pachystachys shoot forests and bamboo farmers to improve their income

The C825T Polymorphism of the G-Protein β3 Gene as a Risk Factor for Depression: A Meta-Analysis

<div><p>Background</p><p>TheG-protein β3 gene (GNβ3) has been implicated in psychiatric illness through its effects upon intracellular transduction of several neurotransmitter receptors. Multiple studies have investigated the relationship of the C825T polymorphism of the GNβ3 gene (GNβ3 C825T) to depression and antidepressant response. However, the relationship between GNβ3 C825T and depression remains inconsistent. Therefore, here we performed a meta-analysis to investigate the role of GNβ3 C825Tin depression risk.</p><p>Methods</p><p>Published case-control studies examining the association between GNβ3 C825T and depression were systematically searched for through several electronic databases (PubMed, Scopus, Science Direct, Springer, Embase, psyINFO, and CNKI). The association between GNβ3 C825T and depression risk were assessed by odd ratios (ORs) and their 95% confidence intervals (CIs) for each study. Pooled ORs were constructed for allele contrast (C versus T), homozygote (CC versus TT) model, heterozygote (CC versus CT) model, dominant model (CC + CT versus TT), and recessive (CC versus TT+CT) model. In order to evaluate possible biases, a sensitivity analysis was conducted by sequential deletion of individual studies in an attempt to assess the contribution of each individual dataset to the pooled OR.</p><p>Results</p><p>Nine studies, including 1055 depressed patients and 1325 healthy controls, were included. A significant association between GNβ3 C825Tand depression was found to exist, suggesting that the T-allele of GNβ3 C825Tcan increase susceptibility to depression. After stratification by ethnicity, the same association was found in the Asian subpopulation, but not the Caucasian subpopulation.</p><p>Conclusions</p><p>This is the first meta-analysis to reveal a relationship between GNβ3 C825T and depression. Asian T-allele carriers of GNβ3 C825T appear to be more susceptible to depression.</p></div