Research on the Achievements, Experience and Innovation Path of China’s Green Sharing Economy in the New Era

The Green Sharing Economy represents a novel economic paradigm emergent under the auspices of global technological revolutions and industrial transformation. This paradigm facilitates optimal resource allocation, establishing precise alignments between “demand pools” and “supply pools.” It concurrently enhances ecological environments, creating a harmonious interplay between lush landscapes and prosperous economies, while expanding employment opportunities, and fostering synergistic developments in mass entrepreneurship and innovation. Within the framework of the New Era, China’s Green Sharing Economy has experienced rapid ascension. This growth fundamentally stems from the continuous refinement of top-level designs by the Chinese government, guiding high-quality development in the Green Sharing Economy. Initiatives include the advancement of administrative streamlining and decentralization, creating a conducive business environment; the acceleration of information technology development, eliminating connectivity barriers; and significant investment in digital talent cultivation, providing the necessary human capital foundation. At this historical juncture, it is imperative to expedite the construction of a trust system for the Green Sharing Economy, continuously optimize its platform technology, constantly improve its regulatory framework, and actively expand its global market presence

N,N′-p-Phenyl­enediisonicotinamide monohydrate

The organic mol­ecule of the title compound, C18H14N4O2·H2O, lies on a center of inversion located at the centre of the central phenyl­ene ring. There are two half-molecules in the asymmetric unit. In the crystal, the mol­ecules are linked through by N—H⋯O and O—H⋯N hydrogen bonds involving the water mol­ecule, forming a layer structure. The layers inter­act by π–π inter­actions between the aromatic rings

Molecular Cloning and Characterization of Babesia orientalis

Babesiosis caused by Babesia orientalis is one of the most prevalent infections of water buffalo transmitted by Rhipicephalus haemaphysaloides causing a parasitic and hemolytic disease. The organelles proteins localized in apical membrane especially rhoptries neck and microneme protein form a complex called moving junction important during invasion process of parasites belonging to apicomplexan group, including Babesia species. A truncated fragment coding a 936 bps fragment was cloned in pMD-19T and subcloned into pET32 (a)+ expression vector, expressed in E. coli BL21. Purified recombinant BoRON2 was used to produce polyclonal antibody against BoRON2. Here, we identified the full sequence of gene encoding the rhoptry neck 2 protein that we named BoRON2 which is 4035 bp in full-length open reading frame without introns, encoding a polypeptide of 1345 amino acids. Western blot of rBoRON2 probed with buffalo positive serum analysis revealed a band of around 150 kDa in parasite lysates, suggesting an active involvement during invasion process. These findings most likely are constructive in perspective of ongoing research focused particularly on water buffalo babesiosis prevention and therapeutics and globally provide new information for genes comparative analysis

Effects of Organizational Leadership on Project Citizenship Behavior and Management Performance in Complex Construction Projects

Organizational leadership is a key factor affecting the management performance of complex construction projects, but seldom have studies attempted to explore the effect mechanisms of organizational leadership on the project management performance, especially the mediating role of project citizenship behavior. The purpose of this study is to fill this gap by investigating the effects of organizational leadership on project citizenship behavior and management performance in complex construction projects. The theoretical model is constructed based on a literature review, and exploratory factor analyses (EFA) are performed on 169 valid questionnaires collected to measure organizational leadership, then partial least squares-structural equation modeling (PLS-SEM) is used to test the hypotheses. The results show that (i) organizational leadership is measured as vision guiding, context interacting, team building, and systems thinking; (ii) vision guiding and context interacting have both direct and indirect effects on the project management performance, and team building can only improve the project management performance by influencing the project citizenship behavior, whereas systems thinking has no significant effect on project citizenship behavior and the project management performance in complex construction projects; (iii) and project citizenship behavior partly mediates the influence of organizational leadership on the project management performance, and the effect of organizational leadership on the project management performance is more realized through the mediating role of project citizenship behavior. The results have a significant theoretical and practical significance for improving the project management performance

The Corrosion Behavior of Ti-Based Metallic Glass Matrix Composites in the H2SO4 Solution

In-situ metallic glass matrix composites (MGMCs) are a new kind of promising structural engineering material. In this study, a comprehensive study about the corrosion behavior of in-situ Ti46Zr20V12Cu5Be17 MGMCs versus their size was conducted. It is interesting to find that the large sample exhibits a more excellent corrosion resistance than the small sample in the 10% H2SO4 solution both in chemical and electrochemical tests at room temperature. Furthermore, the results obtained from X-ray photoelectron spectra (XPS) demonstrate that the quality of the passive film formed on the large-sized sample is indeed superior to the small-sized sample. Based on (scanning electron microscope) SEM observation, the possible mechanisms of the observed phenomenon have been discussed in the present study

Isothermal Storage Delays the Senescence of Post-Harvest Apple Fruit through the Regulation of Antioxidant Activity and Energy Metabolism

The purpose of this work was to elucidate the influence of TF (5 ± 5 °C, and 5 ± 1 °C) and CT (5 ± 0.1 °C served as an isothermal state) storage environment on the antioxidant ability and energy metabolism in post-harvest apple fruit during storage. Specifically, compared with fruit in TFs groups, the quality attributes of apples in the CT group, including firmness, fresh weight, contents of SSC, and TA were maintained at a higher level. In addition, fruit stored in the CT environment revealed a suppressed respiration rate and EL, lower MDA, O2·−, and H2O2 accumulation but increased the activities of SOD, CAT, APX, and GR. At the end of storage, the SOD, CAT, APX, and GR activities of fruit in the CT group were 38.14%,48.04%, 115.29%, and 34.85% higher than that of the TF5 group, respectively. Fruit in the CT environment also revealed higher AsA, GSH, total phenols, and total flavonoid content. In addition, fruit stored in the CT environment maintained higher ATP content, EC, and more active H+-ATPase, Ca2+-ATPase, CCO, and SDH. At the end of storage, the SDH and CCO activities of fruit in the TF0.1 group were 1.74, and 2.59 times higher than that in the TF5 group, respectively. Taken together, we attributed the fact that a constant temperature storage environment can retard the fruit senescence to the enhancement of antioxidant capacities and maintaining of higher energy status in apple fruit

Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR

In order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, and chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) of specific genomic regions from five selected species and specificity and amplification efficiency were determined. The mixed DNA template with an equal copy number was used for detecting the accuracy and limit of multiplex PCR. The results showed that the primers and probes of the five selected species had good specificity with the minimum number of detection copies: 0.15 copies/µL beef (Bos taurus), 0.28 copies/μL duck (Anas platyrhynchos), 0.37 copies/μL pork (Sus scrofa), 0.39 copies/μL chicken (Gallus gallus), and 0.41 copies/μL mutton (Ovis aries), respectively. The five sets of primers and probes could quickly judge whether the specified meat components existed in the food commodities

First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs