2 research outputs found
Human Papillomavirus (HPV) Detection Using In Situ Hybridization in Histologic Samples Correlations With Cytologic Changes and Polymerase Chain Reaction HPV Detection
Although in situ hybridization (ISH) and polymerase chain reaction (PCR)
have extensively been used on cytology specimens, there have been
limited reports of the usefulness of these techniques in relation to
confirmed histologic findings. In this study, we used PCR and ISH to
detect human papillomavirus (HPV) in cytologic and histologic specimens,
respectively. By using positive and negative likelihood ratios, we
attempted to identify any predictive role of ISH testing alone or in
combination with PCR for the development of high-grade histologic
lesions (cervical intraepithelial neoplasia [CIN] 2+). In our study,
ISH was a useful method for detection of HP V. even in a large fraction
of samples with normal cytologic or biopsy findings. We suggest that
when used together and evaluated in conjunction with histologic
sections, ISH is a useful tool for ancillary molecular testing of HPV
infection in cervical lesions, especially in CIN 2+ histological lesions
where its analytic sensitivities and specificities were as good as those
of PCR testing