A snoRNA modulates mRNA 3' end processing and regulates the expression of a subset of mRNAs.

mRNA 3' end processing is an essential step in gene expression. It is well established that canonical eukaryotic pre-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-acting proteins. However, it is unknown whether RNAs play any role in this process. Unexpectedly, we found that a subset of small nucleolar RNAs (snoRNAs) are associated with the mammalian mRNA 3' processing complex. These snoRNAs primarily interact with Fip1, a component of cleavage and polyadenylation specificity factor (CPSF). We have functionally characterized one of these snoRNAs and our results demonstrated that the U/A-rich SNORD50A inhibits mRNA 3' processing by blocking the Fip1-poly(A) site (PAS) interaction. Consistently, SNORD50A depletion altered the Fip1-RNA interaction landscape and changed the alternative polyadenylation (APA) profiles and/or transcript levels of a subset of genes. Taken together, our data revealed a novel function for snoRNAs and provided the first evidence that non-coding RNAs may play an important role in regulating mRNA 3' processing

Heterologous expression and characterization of a malathion-hydrolyzing carboxylesterase from a thermophilic bacterium, Alicyclobacillus tengchongensis

A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine hydrolases, including Ser204, Glu325, and His415 as a catalytic triad, as well as type-B carboxylesterase serine active site (FGGDPENITIGGQSAG) and type-B carboxylesterase signature 2 (EDCLYLNIWTP). The purified enzyme exhibited optimum activity with β-naphthyl acetate at 60 °C and pH 7 as well as stability at 25 °C and pH 7. One unit of the enzyme hydrolyzed 5 mg malathion l(−1) by 50 % within 25 min and 89 % within 100 min. The enzyme strongly degraded malathion and has a potential use for the detoxification of malathion residues

Analysis of Factors that Influenced the Participation Rates in Upper-secondary Vocational Schools in China

Vocational education and training has been treated as a good pathway to smoothly promote youth from school to the workplace. However, for many reasons, the vocational education in Asian countries is not as popular as general education, and the participation rate in vocational schools is normally lower than it is in general schools. This paper builds a theoretical model about the factors that influence the participation rate in Chinese upper-secondary vocational schools and explains the possible related indicators through a regression analysis method. The results show that the most positive factor is related to the number of male students in vocational schools, followed by the number of female vocational teachers. Other staff in vocational schools also have a positive effect, while the youth unemployment rate and the number of men vocational teachers both have negative relationships with the participation rate. In China, it is possible to improve the rates of new entrants into vocational schools from the aspects of the gender issues, the training methods for vocational teachers, and the social recognition of vocational education

Paradigm model of online learning experience during COVID-19 crisis in higher education

The study presents qualitative research on university students' online learning experience process at the higher education level. It used a grounded theory through semi-structured in-depth interviews with 25 university students for framing and structuring the paradigm model of university students' online learning experiences. A paradigm model illustrating this developmental process is presented, which includes the casual and contextual conditions that caused and evolved the central phenomenon for their online learning, the strategies used by the students to overcome external/internal disturbances in continuing their learning process, the conditions that helped/hindered those strategies, and the consequences. Finally, the following meaning units were discovered through the preceding paradigm model. The students started learning with a negative impression of unfamiliar learning methods such as online learning, but over time, they improved their self-management abilities and gained a broader understanding of self-discipline. In the midst of this, competence in digital literacy according to generational differences was also discovered as one of the factors affecting learning, forming a virtual community, and increasing personal learning through online interaction. Finally, the implications of the overall learning ecology for the future role of online learning were also considered. Based on this analysis of students' online learning experiences, the direction for online learning was suggested

Research on cryptographic properties of a new S-box based on cellular automaton

A new S-box based on cellular automata was found by experiments.The permutation properties of the S-box were analyzed,which proved that the S-box was a permutation only when the size of lattice was 5.Then the relation between the nontrivial difference transition probability of the S-box and the rank of the difference matrix was proved by constructing the difference matrix.And it was proved that the cyclic shift of input differential would not change the corresponding nontrivial differential transition probability,and obtained the sufficient and necessary conditions about the maximum and minimum nontrivial differential transition probabilities when the size of lattice was 5.Then the problem of the difference distribution of the S-box in this situation is completely solved

KINEMATICS ANALYSIS OF CHINESE VAULTING HORSE ATHLETE YANG YAHONG'S ACTION OF "HANDSPRING WITH 90&ORDM-180&ORDM-LAYOUT BACK DOUBLE TWIST"

"Handspring with 90&ordm-180&ordm-layout back double twists" is the new developed kind of action in the women gymnastics championship in recent years. Yang Yahong is the only one that can complete this action. With the method of sports biomechanics, the kinematics characteristic of her action will be analyzed and her advantage and disadvantage will be exposed. The finding shows that her running up is fast, the angle speed of the handspring in the second flight is swift and the pose in space is excellent. However, the amount of time of pushing horse is over long and the height of the 'handspring in the second flight is not enough

Testing Together Behaviors in Secondary Distribution of HIV/Syphilis Self-testing Program Among Men Who have Sex with Men in China

HIV self-testing (HIVST) is recommended as a promising way to increase HIV testing uptake among MSM. MSM sometimes used HIVST kits together with their sexual partners or friends. However, limited data was reported on MSM’s testing together behaviors with sexual partners and non-sexual contacts. Data were collected among MSM in China from June 2018 to June 2019. Eligible participants (referred to as “index participants”) finished a baseline survey and applied for HIVST kits. They were encouraged to distribute the kits to other people (referred to as “alters”). Index participants finished a 3-month follow-up survey on the distribution and usage of the kits. Alters finished an online survey on the usage of HIVST kits after they returned the photographed testing results. Results were reported based on index participants and alters, respectively. Based on follow-up data, 138 index participants successfully motivated others for HIVST, most of them (77.5%) tested together with at least one alter. Around half of alters (52.3%) reported testing together with index participants. Index participants distributed more HIVST kits to friends than sexual partners. MSM who had ever tested for HIV were more likely to test together. Our study demonstrated that the testing together behaviors during HIVST distribution among sexual partners and social network contacts were common. The social network-based approach is essential in promoting testing together and HIV status disclosure among MSM

RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma

Ferroptosis is a distinct iron-dependent programmed cell death and plays important roles in tumor suppression. However, the regulatory mechanisms of ferroptosis need further exploration. RUNT-related transcription factor 2 (RUNX2), a transcription factor, is essential for osteogenesis. RUNX2 has two types of transcripts produced by two alternative promoters. In the present study, we surprisingly find that RUNX2 isoform II is a novel ferroptosis and apoptosis suppressor. RUNX2 isoform II can bind to the promoter of peroxiredoxin-2 (PRDX2), a ferroptosis inhibitor, and activate its expression. Knockdown of RUNX2 isoform II suppresses cell proliferation in vitro and tumorigenesis in vivo in oral squamous cell carcinoma (OSCC). Interestingly, homeobox A10 (HOXA10), an upstream positive regulator of RUNX2 isoform II, is required for the inhibition of ferroptosis and apoptosis through the RUNX2 isoform II/PRDX2 pathway. Consistently, RUNX2 isoform II is overexpressed in OSCC, and associated with OSCC progression and poor prognosis. Collectively, OSCC cancer cells can upregulate RUNX2 isoform II to inhibit ferroptosis and apoptosis and facilitate tumorigenesis through the novel HOXA10/RUNX2 isoform II/PRDX2 pathway

XTQA: Span-Level Explanations of the Textbook Question Answering

Textbook Question Answering (TQA) is a task that one should answer a diagram/non-diagram question given a large multi-modal context consisting of abundant essays and diagrams. We argue that the explainability of this task should place students as a key aspect to be considered. To address this issue, we devise a novel architecture towards span-level eXplanations of the TQA (XTQA) based on our proposed coarse-to-fine grained algorithm, which can provide not only the answers but also the span-level evidences to choose them for students. This algorithm first coarsely chooses top $M$ paragraphs relevant to questions using the TF-IDF method, and then chooses top $K$ evidence spans finely from all candidate spans within these paragraphs by computing the information gain of each span to questions. Experimental results shows that XTQA significantly improves the state-of-the-art performance compared with baselines. The source code is available at https://github.com/keep-smile-001/opentqaComment: 10 page