The Chinese pine genome and methylome unveil key features of conifer evolution

Conifers dominate the world's forest ecosystems and are the most widely planted tree species. Their giant and complex genomes present great challenges for assembling a complete reference genome for evolutionary and genomic studies. We present a 25.4-Gb chromosome-level assembly of Chinese pine (Pinus tabuliformis) and revealed that its genome size is mostly attributable to huge intergenic regions and long introns with high transposable element (TE) content. Large genes with long introns exhibited higher expressions levels. Despite a lack of recent whole-genome duplication, 91.2% of genes were duplicated through dispersed duplication, and expanded gene families are mainly related to stress responses, which may underpin conifers' adaptation, particularly in cold and/or arid conditions. The reproductive regulation network is distinct compared with angiosperms. Slow removal of TEs with high-level methylation may have contributed to genomic expansion. This study provides insights into conifer evolution and resources for advancing research on conifer adaptation and development

Echoes of the Roots

Echoes of the Roots is a spellbinding journey set on an alternate Earth where ancient myths clash with futuristic technology. In this action-packed adventure game, you will engage in fast-paced combat that requires you to make tactical decisions on the fly and discover a variety of weapon combos to create unique playstyles.Get ready to step into the shoes of an intrepid explorer, utilizing a versatile weapon system to conquer consciousness-awakening plant monsters and uncover secrets of a forgotten past.https://source.sheridancollege.ca/swfaad_capstone_game_design_2024/1047/thumbnail.jp

A Fully Coupled Hydromechanical Model for CO2 Sequestration in Coal Seam: the Roles of Multiphase Flow and Gas Dynamic Diffusion on Fluid Transfer and Coal Behavior

CO2 sequestration in coal seam has proved to be an effective way for reducing air pollution caused by greenhouse gases. A study on the rules of fluid transfer and reliability of CO2 storage during gas injection is necessary for the engineering application. However, the clarification of multifield coupling in long-term CO2 sequestration is the difficulty to solve the aforementioned problem. Previous investigations on the coupled model for CO2 storage in coal seam were not exactly comprehensive; for example, the multiphase flow in the fracture and the nonlinear behavior of gas diffusion were generally neglected. In this paper, a new multistage pore model of the coal matrix and the corresponding dynamic diffusion model were adopted. Meanwhile, the CO2-induced coal softening and the CO2-water two-phase flow in coal fracture were also taken into account. Subsequently, all the mentioned mechanisms and interactions were embedded into the coupled hydromechanical model, and this new fully coupled model was well verified by a set of experimental data. Additionally, through the model application for long-term CO2 sequestration, we found that the stored CO2 molecules are mainly in an adsorbed state at the early injection stage, while with the continuous injection of gas, the stored CO2 molecules are mainly in a free state. Finally, the roles of multiphase flow and gas dynamic diffusion on fluid transfer and coal behavior were analyzed. The results showed that the impact of multiphase flow is principally embodied in the area adjacent to the injection well and the coal seam with lower initial water saturation is more reliable for CO2 sequestration, while the impact of gas dynamic diffusion is principally embodied in the area far away from the injection well, and it is safer for CO2 sequestration in coal seam with greater attenuation coefficient of CO2 diffusion

Meliacarpinin-Type Limonoids from the Bark of Melia toosendan

Three new meliacarpinin-type limonoids, toosendanes A–C (1–3), along with three, known meliacarpinins (4–6) were isolated from the bark of Melia toosendan. Their structures, along with their absolute configurations, were elucidated, based on detailed analyses. These included HRESIMS and 1D/2D-NMR, modified Mosher’s method, and electronic circular dichroism (ECD). Limonoids 2 and 3 showed moderate inhibitory activity on LPS-activated, RAW 264.7 macrophages

Examining the Effects of Hydropower Station Construction on the Surface Temperature of the Jinsha River Dry-Hot Valley at Different Seasons

On the completion of a large-scale hydropower station, the change of the water area can cause a corresponding change of local weather. To examine such changes, this paper analyzed the effect of the reservoir in the head area of the Xiluodu hydropower station based on the temperature data of MODIS MYD11A2. The temperature differences (TD) between various locations in the study area and the reservoir were calculated to explore the TD in different seasons. The reservoir effect change intensity (RECI) was established to explore the impact of the reservoir on local weather changes in different flood seasons. The combination of the TD and RECI was applied to explore the role of the hydropower station in regulating the temperature of the surrounding reservoir. The results showed the following: (1) after hydropower station construction (HSC), the TD in the valleys decreased and the TD in the dry season was lower than that in the wet season; (2) the RECI had different distribution characteristics in different flood seasons of the reservoir, and the RECI was stronger in the wet season than that in the dry season; and (3) unlike in the plains, cooling and warming effects existed simultaneously in different parts of the mountains

Preventive Effects of Fluoro-Substituted Benzothiadiazole Derivatives and Chitosan Oligosaccharide against the Rice Seedling Blight Induced by Fusarium oxysporum

Rice seedling blight, caused by Fusarium oxysporum, significantly affects global rice production levels. Fluoro-substituted benzothiadiazole derivatives (FBT) and chitosan oligosaccharide (COS) are elicitors that can enhance plant resistance to pathogen infection. However, there is a lack of information regarding FBT and COS used as elicitors in rice seedlings blight. Therefore, the aim of this study was to evaluate the effect of FBT and COS treatments on rice seedling blight and elucidate the molecular mechanisms of the two elicitors for inducing resistance using proteomic technique. Results indicated that FBT and COS significantly reduced the disease incidence and index, and relived the root growth inhibition caused by F. oxysporum (p < 0.05). Biochemical analyses demonstrated that these two elicitors effectively enhanced activities of defense enzymes. Moreover, the proteomic results of rice root tissues disclosed more differentially expressed proteins in diterpenoid biosynthesis pathway that were particularly stimulated by two elicitors compared to the other pathways studied, resulting in the accumulation of antimicrobial substance, momilactone. Findings of this study could provide sound theoretical basis for further applications of FBT and COS used as rice elicitors against seedling blight

Porcine induced pluripotent stem cells require LIF and maintain their developmental potential in early stage of embryos.

Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet the stringent requirements of pluripotency. Here, we report the generation of piPS cells from fibroblasts using retrovirus carrying four mouse transcription factors (mOct4, mSox2, mKlf4 and mc-Myc, 4F). Multiple LIF-dependent piPS cell lines were generated and these cells showed the morphology similar to mouse embryonic stem cells and other pluripotent stem cells. In addition to the routine characterization, piPS cells were injected into porcine pre-compacted embryos to generate chimera embryos and nuclear transfer (NT) embryos. The results showed that piPS cells retain the ability to integrate into inner and outer layers of the blastocysts, and support the NT embryos development to blastocysts. The generations of chimera embryos and NT embryos derived from piPS clones are a practical means to determine the quality of iPS cells ex vivo