Autoimmunity and effector recognition in Arabidopsis thaliana can be uncoupled by mutations in the RRS1‐R immune receptor

Plant nucleotide-binding leucine-rich repeat (NLR) disease resistance proteins recognize specific pathogen effectors and activate a cellular defense program. In Arabidopsis thaliana (Arabidopsis), Resistance to Ralstonia solanacearum 1 (RRS1-R) and Resistance to Pseudomonas syringae 4 (RPS4) function together to recognize the unrelated bacterial effectors PopP2 and AvrRps4. In the plant cell nucleus, the RRS1-R/RPS4 complex binds to and signals the presence of AvrRps4 or PopP2. The exact mechanism underlying NLR signaling and immunity activation remains to be elucidated. Using genetic and biochemical approaches, we characterized the intragenic suppressors of sensitive to low humidity 1 (slh1), a temperature-sensitive autoimmune allele of RRS1-R. Our analyses identified five amino acid residues that contribute to RRS1-R SLH 1 autoactivity. We investigated the role of these residues in the RRS1-R allele by genetic complementation, and found that C15 in the Toll/interleukin-1 receptor (TIR) domain and L816 in the LRR domain were also important for effector recognition. Further characterization of the intragenic suppressive mutations located in the RRS1-R TIR domain revealed differing requirements for RRS1-R/RPS4-dependent autoimmunity and effector-triggered immunity. Our results provide novel information about the mechanisms which, in turn, hold an NLR protein complex inactive and allow adequate activation in the presence of pathogens

ABSORPTION LINE GRADIENTS IN THE SPECTRUM OF AN ELLIPTICAL GALAXY NGC 5864A

The archival long-slit spectra, covering the wavelength range 4050 ~ 5150 Å, have been used to investigate the radial behavior of absorption line fea-tures (G4300, Fe4383, Ca4455, Fe4531, and Hβ) of an elliptical galaxy NGC 5846A. The heliocentric recession velocity of NGC 5846 has been derived as 1949±87 km/s. Fe absorption lines of NGC 5846A show significant radial gradients with the mean slope of ∆/∆(r")=-0.863±0.202. There is also a significant radial gradient of G band with a slope of -1.109±0.098. On the other hand, no radial gradients has been detected on the Ca4455 and Hβ absorptions of NGC 5846A. A metallicity gradient, which is derived from the Fe line gradients, is similar to the abundance gradient predicted by Larson's (1975) dissipative models for the formation of elliptical galaxies. We also note that a galaxy-galaxy interaction could affect the line gradients of NGC 5864A

Color Gradient in the King Type Globular Cluster NGC 7089

We use BV CCD images to investigate the reality of the color gradient within a King type globular cluster NGC 7089. Surface photometry shows that there is a strong radial color gradient in the central region of the cluster in the sense of bluer center with the amplitude of -0.39 +/- 0.07 mag/arcsec2 in (B - V). In the outer region of the cluster, however, the radial color gradient shows a reverse case, i.e., redder toward the center. (B - V) color profile which was derived from resolved stars in VGC 7089 field also shows a significant color gradient in the central region of the clusters, indicating that lights from the combination of red giant stars and blue horizontal branch stars cause the radial color gradient. Color gradient of the outer region of NGC 7089 may be due to the unresolved background of the cluster. Similar color gradients in the central area of clusters have been previously observed exserved exclusively in highly concentrated systems classified as post core collapse clusters. We caution, however, to confirm the reality of the color gradient from resolved stars, we need more accurate imaging data of the cluster with exceptional seeing condition because the effect of completeness correlates with local density of stars

Review of MXene-based nanocomposites for photocatalysis

Since multilayered MXenes (Ti3C2Tx, a new family of two-dimensional materials) were initially introduced by researchers at Drexel University in 2011, various MXene-based nanocomposites have received increased attention as photocatalysts owing to their exceptional properties (e.g., rich surface chemistry, adjustable bandgap structures, high electrical conductivity, hydrophilicity, thermal stability, and large specific surface area). Therefore, we present a comprehensive review of recent studies on fabrication methods for MXene-based photocatalysts and photocatalytic performance for contaminant degradation, CO2 reduction, H-2 evolution, and N-2 fixation with various MXene-based nanocomposites. In addition, this review briefly discusses the stability of MXene-based nanophotocatalysts, current limitations, and future research needs, along with the various corresponding challenges, in an effort to reveal the unique properties of MXene-based nanocomposites. (C) 2020 Elsevier Ltd. All rights reserved.N

Modification of the Association between Visual Impairment and Mortality by Physical Activity: A Cohort Study among the Korean National Health Examinees

The association between visual impairment and higher mortality remains unclear. In addition, evidence is lacking on the interaction between visual function and physical activity on mortality. We used data of individuals with no disability or with visual impairment among those who participated in the National Health Screening Program in Korea in 2009 or 2010. We constructed Cox proportional hazard models adjusted for potential confounders to evaluate the independent association between visual impairment and mortality. More severe visual impairment was associated with higher all-cause mortality (p-value for trend = 0.03) and mortality due to cardiovascular diseases (p-value for trend = 0.02) and that due to other diseases (p-value for trend = 0.01). We found an interaction on an additive scale between visual impairment and no physical activity on all-cause mortality (relative excess risk due to interaction = 1.34, 95% confidence interval: 0.37, 2.30, p-value = 0.01). When we stratified the study population by physical activity, the association between visual impairment and mortality was only found among individuals who did not engage in regular physical activity (p-value for trend = 0.01). We found an independent association between visual impairment and mortality and modification of this association by physical activity

Blood Test for Breast Cancer Screening through the Detection of Tumor-Associated Circulating Transcripts

Liquid biopsy has been emerging for early screening and treatment monitoring at each cancer stage. However, the current blood-based diagnostic tools in breast cancer have not been sufficient to understand patient-derived molecular features of aggressive tumors individually. Herein, we aimed to develop a blood test for the early detection of breast cancer with cost-effective and high-throughput considerations in order to combat the challenges associated with precision oncology using mRNA-based tests. We prospectively evaluated 719 blood samples from 404 breast cancer patients and 315 healthy controls, and identified 10 mRNA transcripts whose expression is increased in the blood of breast cancer patients relative to healthy controls. Modeling of the tumor-associated circulating transcripts (TACTs) is performed by means of four different machine learning techniques (artificial neural network (ANN), decision tree (DT), logistic regression (LR), and support vector machine (SVM)). The ANN model had superior sensitivity (90.2%), specificity (80.0%), and accuracy (85.7%) compared with the other three models. Relative to the value of 90.2% achieved using the TACT assay on our test set, the sensitivity values of other conventional assays (mammogram, CEA, and CA 15-3) were comparable or much lower, at 89%, 7%, and 5%, respectively. The sensitivity, specificity, and accuracy of TACTs were appreciably consistent across the different breast cancer stages, suggesting the potential of the TACTs assay as an early diagnosis and prediction of poor outcomes. Our study potentially paves the way for a simple and accurate diagnostic and prognostic tool for liquid biopsy