Individual Development of Professionalism in Educational Peer Group Supervision: A Multiple Case Study of GPs

Background. Research has shown that peer-group supervision can strengthen GPs’ professionalism, but little is known about the individual learning processes. To establish professionalism beyond professional behaviour, identity and idealism need to be included. The inner attitudinal values of professionalism within the individual are, however, difficult to assess. Aim. On the basis of a multiple case study, this paper describes the process of professional learning and challenges for individual GPs, as they take part in supervision groups focusing on children cases. Methods and Results. By using a two-dimensional theoretical model, it is shown that all GPs developed their professional behaviour, and many of them strengthened their professional identity in this domain towards a changed professionalism. Most participants emphasized the positive experience of sharing worries with families indicating care and interest. Some participants learning processes were very linear/convergent; others were complex/divergent—starting out with a relatively simple objective, realizing how multifaceted the issue was after the first year leading to a final development of new perspectives or action possibilities. Conclusion. The composition of supervision groups, as well as the professional background of the supervisor, may play a significant role in the development of professional behaviour and professionalism.Peer Reviewe

Ag–ZnO Nanocomposites as a 3D Metal-Enhanced Fluorescence Substrate for the Fluorescence Detection of DNA

Fluorescence enhancement, including metal-enhanced fluorescence (MEF), has been widely explored in the field of biosensor technology. Two- and three-dimensional (2D and 3D) MEF substrate approaches have been developed for biosensors to detect target biomarkers such as genes and proteins. However, several issues remain, not least poor reproducibility caused by limited recognition and diffusion of biomolecules among target molecules, biological probes, and MEF substrates in aqueous environments. Here, an Ag–ZnO nanocomplex as a 3D MEF substrate was developed in two steps: hydrothermal synthesis of ZnO nanowire (NW) as a building block of Ag nanoparticles (NPs), and photochemical deposition of AgNPs on the ZnO NW. Fluorescence enhancement on Ag–ZnO nanocomposites (NCs) compared with the glass substrate was 14.8-, 16.9-, 11.4-, and 14.2-fold in the presence of 10, 20, 40, and 60 base pair (bp) double-stranded (ds) DNA, respectively. The MEF effects on glass, ZnO NW, and Ag–ZnO NCs were analyzed by measuring the fluorescence lifetime of fluorescein (FAM)-labeled dsDNA, and the average lifetimes were 9.76, 9.34, and 7.86 ns, respectively. Ag–ZnO NCs showed a 50-fold higher sensitivity for the detection of dsDNA than Ag nanoisland film (AgNIF) as a 2D MEF substrate. Therefore, Ag–ZnO NCs may have potential as a 3D MEF substrate. The MEF phenomenon might involve broad-range interactions between the surface of the metal nanostructure on Ag–ZnO NCs and fluorophores in various scenarios. Our Ag–ZnO NCs could facilitate the development of sensitive MEF-based biosensors for DNA detection

Additional file 2: of Nodding behavior couples to vigilance fluctuation in a high-calorie diet model of drowsiness

Figure S2. Analysis of HF effects on drowsy states including nodding behavior. (a) The average length of nodding episode in the no-food and high-fat food groups (unpaired t-test, p = 0.887, no food n = 2 and high-fat n = 4, n.s. indicates ‘not significant’). All error bars represent s.e.m. (b) The mean duration of no food and high-fat food groups during Nd (unpaired t-test, p = 0.886) and Non-Nd states (unpaired t-test p = 0.895, no food n = 2 and high-fat n = 4). All error bars represent s.e.m. (PDF 88 kb

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Additional file 1: Table S1. Intergenic regions of various constitutive promoters in C. amalonaticus Y19

Enhancing Surface-Enhanced Raman Scattering Intensity through Light Diffuse Reflection on Ag/ZnO Nanowire Arrays

In this study, we present a new strategy to enhance the intensity of surface-enhanced Raman scattering (SERS) signals by using three-dimensional aggregated silver nanoparticles (AgNPs) on the zinc oxide nanowire arrays (Ag/ZnO NWA). The ZnO NWA acts as a light-scattering substrate, providing multidirectional incident light and reflecting a portion of the Raman scattered light, resulting in an improved SERS signal intensity. The composite nanowire structure of the Ag/ZnO NWA was successfully fabricated, and 400 nm ZnO nanowires in the Ag/ZnO NWA exhibit optimal diffuse light reflection, resulting in a significant improvement of the SERS signal intensity. The SERS enhancement factor (EF) for the Ag/ZnO NWA was determined to be EF (ISERS/NSERS)/(IRaman/NRaman) = 3.5 × 106. This substrate generated a ∼7.9-fold increased SERS signal compared to the preaggregated AgNPs in the absence of the ZnO NWA. The detection limit of the Ag/ZnO NWA was estimated by measuring SERS spectra of rhodamine B, enabling discrimination down to 10–15 M. Our approach inducing multidirectional diffuse reflection light to SERS hot spots provides a simple and efficient strategy to enhance SERS signal intensity, with broad implications for other spectroscopic applications

MOESM1 of Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system

Additional file 1. Additional figures and table. Table S1. Strains and plasmid used in this study. Fig. S1. Cell growth profile of L17W in BES and non-BES (A) and L17K in BES and non-BES (B) and pHprofile of L17W in BES and non-BES (C) and L17K in BES and non-BES (D). Fig. S2. Metabolites profile of L17W (A) and L17K (B) in BES and L17W (C) and L17K (D) in non-BES

Additional file 1: of Comparison of intraoperative handling and wound healing between (NEOSORBÂŽ plus) and coated polyglactin 910 suture (NEOSORBÂŽ): a prospective, single-blind, randomized controlled trial

Raw data of study; Raw data of Comparison Between NEOSORB Plus and NEOSORB. (XLSX 39 kb

Additional file 3: Table S3. of Whole genome sequence analysis of the TALLYHO/Jng mouse

Classification of TH indels by sets of SO terms associated with the indel. (XLSX 10 kb

Additional file 2: of The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function

2-D PAGE of the S. aureus Mu50 (WT) and sav1322 mutant strains. (DOCX 568 kb

Additional file 1: of The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function

Colony forming units (CFU) of the S. aureus Mu50 (WT), sav1322 mutant, and complementation (Com) strains at 37 °C, 46 °C, 25 °C. (DOCX 22 kb