11 research outputs found
rMAPS: RNA map analysis and plotting server for alternative exon regulation.
RNA-binding proteins (RBPs) play a critical role in the regulation of alternative splicing (AS), a prevalent mechanism for generating transcriptomic and proteomic diversity in eukaryotic cells. Studies have shown that AS can be regulated by RBPs in a binding-site-position dependent manner. Depending on where RBPs bind, splicing of an alternative exon can be enhanced or suppressed. Therefore, spatial analyses of RBP motifs and binding sites around alternative exons will help elucidate splicing regulation by RBPs. The development of high-throughput sequencing technologies has allowed transcriptome-wide analyses of AS and RBP-RNA interactions. Given a set of differentially regulated alternative exons obtained from RNA sequencing (RNA-seq) experiments, the rMAPS web server (http://rmaps.cecsresearch.org) performs motif analyses of RBPs in the vicinity of alternatively spliced exons and creates RNA maps that depict the spatial patterns of RBP motifs. Similarly, rMAPS can also perform spatial analyses of RBP-RNA binding sites identified by cross-linking immunoprecipitation sequencing (CLIP-seq) experiments. We anticipate rMAPS will be a useful tool for elucidating RBP regulation of alternative exon splicing using high-throughput sequencing data
Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing
Stem cells are useful for cell replacement therapy. Stem cell differentiation must be monitored thoroughly and precisely prior to transplantation. In this study we evaluated the usefulness of electric cell-substrate impedance sensing (ECIS) for in vitro real-time monitoring of neural differentiation of human mesenchymal stem cells (hMSCs). We cultured hMSCs in neural
differentiation media (NDM) for 6 days and examined the time-course of impedance changes with an ECIS array. We also
monitored the expression of markers for neural differentiation, total cell count, and cell cycle profiles. Cellular expression of
neuron and oligodendrocyte markers increased. The resistance value of cells cultured in NDM was automatically measured in real-time
and found to increase much more slowly over time compared to cells cultured in non-differentiation media. The relatively slow
resistance changes observed in differentiating MSCs were determined to be due to their lower growth capacity achieved by
induction of cell cycle arrest in G0/G1. Overall results suggest that the relatively slow change in resistance values measured by
ECIS method can be used as a parameter for slowly growing neural-differentiating cells. However, to enhance the competence
of ECIS for in vitro real-time monitoring of neural differentiation of MSCs, more elaborate studies are needed
Culprit of diffusion-weighted image in acute vestibular syndrome
OAIID:oai:osos.snu.ac.kr:snu2015-01/102/0000004487/3ADJUST_YN:YEMP_ID:A075641DEPT_CD:801CITE_RATE:1.447FILENAME:jeongsh-acute vestibular syndrome-neurol sci-2015.pdfDEPT_NM:ģķź³¼SCOPUS_YN:YCONFIRM:
rMAPS: RNA map analysis and plotting server for alternative exon regulation
RNA-binding proteins (RBPs) play a critical role in the regulation of alternative splicing (AS), a prevalent mechanism for generating transcriptomic and proteomic diversity in eukaryotic cells. Studies have shown that AS can be regulated by RBPs in a binding-site-position dependent manner. Depending on where RBPs bind, splicing of an alternative exon can be enhanced or suppressed. Therefore, spatial analyses of RBP motifs and binding sites around alternative exons will help elucidate splicing regulation by RBPs. The development of high-throughput sequencing technologies has allowed transcriptome-wide analyses of AS and RBPāRNA interactions. Given a set of differentially regulated alternative exons obtained from RNA sequencing (RNA-seq) experiments, the rMAPS web server (http://rmaps.cecsresearch.org) performs motif analyses of RBPs in the vicinity of alternatively spliced exons and creates RNA maps that depict the spatial patterns of RBP motifs. Similarly, rMAPS can also perform spatial analyses of RBPāRNA binding sites identified by cross-linking immunoprecipitation sequencing (CLIP-seq) experiments. We anticipate rMAPS will be a useful tool for elucidating RBP regulation of alternative exon splicing using high-throughput sequencing data
A study on the fabrication of metal microneedle array electrodes for ECG detection based on low melting point BiāInāSn alloys
Abstract This study describes the fabrication and characteristics of microneedle array electrodes (MAEs) using BismuthāIndiumāTin (BiāInāSn) alloys. The MAEs consist of 57 pyramid-shaped needles measuring 340Ā Ī¼m wide and 800Ā Ī¼m high. The fabrication process involved micromolding the alloys in a vacuum environment. Physical tests demonstrated that BiāInāSn MAEs have good mechanical strength, indicating their suitability for successful skin penetration. The electrodeāskin interface impedance test confirmed that BiāInāSn MAEs successfully penetrated the skin. Impedance measurements revealed the importance of insulating the microneedle electrodes for optimal electrical performance, and a UV-curable Polyurethane Acrylate coating was applied to enhance insulation. Electrocardiogram measurements using the BiāInāSn MAEs demonstrated performance comparable to that of traditional Ag/AgCl electrodes, which shows promise for accurate data collection. Overall, the study demonstrates successful, minimally-invasive skin insertion, improved electrical insulation, and potential applications of BiāInāSn microneedle array. These findings contribute to advancements in microneedle technology for biomedical applications
Development of the UKESM-TOPAZ Earth System Model (Version 1.0) and Preliminary Evaluation of its Biogeochemical Simulations
Earth system models (ESMs) comprise various Earth system components and simulate the interactions between these components. ESMs can be used to understand climate feedbacks between physical, chemical, and biological processes and predict future climate. We developed a new ESM, UKESM-TOPAZ, by coupling the UK ESM (UKESM1) and the Tracers of Phytoplankton with Allometric Zooplankton (TOPAZ) biogeochemical module. We then compared the preliminary simulated biogeochemical variables, which were conducted over a period of 70Ā years, using observational and existing UKESM1 model data. Similar to UKESM1, the newly developed UKESM-TOPAZ closely simulated the relationship between the El NiƱo-Southern Oscillation and chlorophyll concentration anomalies during the boreal winter. However, there were differences in the chlorophyll distributions in the eastern equatorial Pacific between the two models, which were due to dissolved iron, as this value was higher in UKESM-TOPAZ than in UKESM1. In a mean field analysis, the distributions of the major marine biogeochemical variables in UKESM-TOPAZ (i.e., nitrate, silicate, dissolved oxygen, dissolved inorganic carbon, and alkalinity) were not significantly different from those of UKESM1, likely because the models share the same initial conditions. Our results indicate that TOPAZ has a simulation performance that does not lag behind UKESM1ās basic biogeochemical model (Model of Ecosystem Dynamics, nutrient Utilisation, Sequestration, and Acidification; MEDUSA). The UKESM-TOPAZ model can simulate the variability of the observed NiƱo 3.4 and 4 indices more closely than UKESM1. Thus, the UKESM-TOPAZ model can be used to deepen our understanding of the Earth system and to estimate ESM uncertainty