Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production

Table S3. Comparison of fed-batch fermentation with EMY-01, EMY-68, EMY-70S, and EMY-70SP using sugarcane molasses

Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering

BACKGROUND: 2,3-Butanediol is a chemical compound of increasing interest due to its wide applications. It can be synthesized via mixed acid fermentation of pathogenic bacteria such as Enterobacter aerogenes and Klebsiella oxytoca. The non-pathogenic Saccharomyces cerevisiae possesses three different 2,3-butanediol biosynthetic pathways, but produces minute amount of 2,3-butanediol. Hence, we attempted to engineer S. cerevisiae strain to enhance 2,3-butanediol production. RESULTS: We first identified gene deletion strategy by performing in silico genome-scale metabolic analysis. Based on the best in silico strategy, in which disruption of alcohol dehydrogenase (ADH) pathway is required, we then constructed gene deletion mutant strains and performed batch cultivation of the strains. Deletion of three ADH genes, ADH1, ADH3 and ADH5, increased 2,3-butanediol production by 55-fold under microaerobic condition. However, overproduction of glycerol was observed in this triple deletion strain. Additional rational design to reduce glycerol production by GPD2 deletion altered the carbon fluxes back to ethanol and significantly reduced 2,3-butanediol production. Deletion of ALD6 reduced acetate production in strains lacking major ADH isozymes, but it did not favor 2,3-butanediol production. Finally, we introduced 2,3-butanediol biosynthetic pathway from Bacillus subtilis and E. aerogenes to the engineered strain and successfully increased titer and yield. Highest 2,3-butanediol titer (2.29 g·l(-1)) and yield (0.113 g·g(-1)) were achieved by Δadh1 Δadh3 Δadh5 strain under anaerobic condition. CONCLUSIONS: With the aid of in silico metabolic engineering, we have successfully designed and constructed S. cerevisiae strains with improved 2,3-butanediol production

6.EMEA International Symposium in Kanazawa, Japan

Inha UniversityProject Number 14404021, Peport of Research Project ; Grant-in-Aid for Scientific Research(B)(2), from April 2002 to March 2006, Edited by Muramoto,Ken-ichiroKamata, NaotoKawanishi, TakuyaKubo, MamoruLiu, JiyuanLee, Kyu-Sung , 人工衛星データ活用のための東アジアの植生調査、課題番号14404021, 平成14年度～平成17年度科学研究費補助金, 基盤研究（B)(2)研究成果報告書, 研究代表者：村本, 健一郎, 金沢大学自然科学研究科教

Comparison of special characteristics of drought-stressed vegetation observed by In-Situ measurements and satellite remote sensing

Inha University2005 International Symposium on Environmental Mornitoring in East Asia -Remote Sensing and Forests-,Hosted The EMEA Project, Kanazawa University 21st=Century COE Program -Environmental Monitoring and Predicition of Long- and Short- Term Dynamics of Pan-Japan Sea Area- ,予稿集, EMEA 2005 in Kanazawa, 国際学術研究公開シンポジウム『東アジアの環境モニタリング』－リモートセンシングと森林－,年月日：200511月28日～29日, 場所：KKRホテル金沢, 金沢大学自然科学研究科, 主催：金沢大学EMEAプロジェクト, 共催：金沢大学21世紀COEプログラム「環日本海域の環境変動と長期・短期変動予測

Hydrated copper and gold monovalent cations: Ab initio study

To understand the hydration phenomena of noble transition metals, we investigated the structures, hydration energies, electronic properties, and spectra of the Cu+(H3O)(1-6) and Au+ (H2O)(1-6) clusters using ab initio calculations. The coordination numbers of these clusters are found to be only two, which is highly contrasted to those of Ag+ (H2O)(n) (which have the coordination numbers of 3-4) as well as the hydrated alkali metal ions (which have the coordination numbers of similar to6). For the possible identification of their interesting hydration structures, we predict their IR spectra for the OH stretch modes. (C) 2005 American Institute of Physics.open384