Substrate entering and product leaving trajectories predict an engulfing dynamic for the major conformational change of the β-lactam acylase

It is still a major challenge to acquire insight into the conformational changes between the ground state and the transition state of an enzyme, although conformational fluctuation within interconverting conformers has been widely investigated (1-4). Here, we utilize different enzymatic reactions in b-lactam acylase to figure out the substrate/product trajectories in the enzyme, thereby probing the overall conformational changes in transition state. First, an auto-proteolytic intermediate of cephalosporin acylase (EC 3.5.1.11) with partial spacer segment was identified. As a final proteolytic step, the deletion of this spacer segment was revealed to be a first-order reaction, suggesting an intramolecular Ntn mechanism for the auto-proteolysis. Accordingly, the different proteolytic sites in the acylase precursor indicate a substrate entering pathway along the spacer peptide. Second, bromoacyl-7ACA can interact with penicillin G acylase (EC 3.5.1.11) in two distinguish aspects, to be hydrolyzed as a substrate analogue and to affinity alkylate the conserved Trpb4 as a product analogue. The kinetic correlation between these two reactions suggests a channel opening from Serb1 to Trpb4, responsible for the main product leaving. These two reaction trajectories relaying at the active centre, together with the crystal structures (5-10), predict an engulfing dynamic involving pocket constriction and channel opening

Improving the security of multiparty quantum secret sharing against Trojan horse attack

We analyzed the security of the multiparty quantum secret sharing (MQSS) protocol recently proposed by Zhang, Li and Man [Phys. Rev. A \textbf{71}, 044301 (2005)] and found that this protocol is secure for any other eavesdropper except for the agent Bob who prepares the quantum signals as he can attack the quantum communication with a Trojan horse. That is, Bob replaces the single-photon signal with a multi-photon one and the other agent Charlie cannot find this cheating as she does not measure the photons before they runs back from the boss Alice, which reveals that this MQSS protocol is not secure for Bob. Finally, we present a possible improvement of the MQSS protocol security with two single-photon measurements and six unitary operations.Comment: 4 pages, 2 figures; The revised version of the paper published in Phys. Rev. A 72, 044302 (2005). A bug is modified and an addendum is adde

Multipartite entanglement purification with quantum nondemolition detectors

We present a scheme for multipartite entanglement purification of quantum systems in a Greenberger-Horne-Zeilinger state with quantum nondemolition detectors (QNDs). This scheme does not require the controlled-not gates which cannot be implemented perfectly with linear optical elements at present, but QNDs based on cross-Kerr nonlinearities. It works with two steps, i.e., the bit-flipping error correction and the phase-flipping error correction. These two steps can be iterated perfectly with parity checks and simple single-photon measurements. This scheme does not require the parties to possess sophisticated single photon detectors. These features maybe make this scheme more efficient and feasible than others in practical applications.Comment: 8 pages, 5 figure

3-Benzyl-6-isopropyl-5-phen­oxy-3H-1,2,3-triazolo[4,5-d]pyrimidin-7(6H)-one

In the title compound, C20H19N5O2, all atoms of the 1,2,3-triazolo[4,5-d]pyrimidine ring system are essentially coplanar [maximum deviation = 0.015 (2) Å], indicating the existence of a conjugate system in which each carbon and nitrogen atom is sp 2 hybridized and ten π electrons (three from carbon atoms and seven from nitrogen atoms) constitute an aromatic heterocycle. The ring system forms dihedral angles of 68.37 (10) and 71.57 (9)° with the phenyl rings. The crystal packing is stabilized by van der Waals inter­actions and intermolecular C—H⋯π interactions

The effectiveness and safety of parathyroid hormone in fracture healing: A meta-analysis

The very large economic and social burdens of fracture-related complications make rapid fracture healing a major public health goal. The role of parathyroid hormone (PTH) in treating osteoporosis is generally accepted, but the effect of PTH on fracture healing is controversial. This meta-analysis was designed to investigate the efficacy and safety of PTH in fracture healing. The EMBASE, PubMed, and Cochrane Library databases were systematically searched from the inception dates to April 26, 2018. The primary randomized clinical trials comparing PTH treatment for fracture healing with placebo or no treatment were identified. We did not gain additional information by contacting the authors of the primary studies. Two reviewers independently extracted the data and evaluated study quality. This meta-analysis was executed to determine the odds ratio, mean difference, standardized mean difference, and 95% confidence intervals with random-effects models. In total, 8 randomized trials including 524 patients met the inclusion criteria. There were significant differences in fracture healing time, pain relief and function improvement. There were no significant differences in the fracture healing rate or adverse events, including light-headedness, hypercalcemia, nausea, sweating and headache, except for slight bruising at the injection site. We determined that the effectiveness and safety of PTH in fracture healing is reasonably well established and credible