Towards a New Classification Scheme of Geothermal Systems in China

ABSTRACT Classification of geothermal systems provides good basis for exploration and assessment of geothermal resources. China is rich in geothermal resources and genetic mechanisms are diverse with complicated geological backgounds. In this paper we propose a new classification which is based on the type of heat source, followed by mechanism of heat transfer. Four types of tectono-genic heat sources are identified and two types of heat transfer mechanisms have been used for the classification. The dominant factor in determining the uneven distribution or accumulation of heat energy in the crust is emphasized. Other influencing factors are used to further classify the resources into subcategories. Geothermal prospects or plays in the Himalayas are considered to be supplied with crustal magmatism/partial melting, caused by collision-induced conversion from mechanical to heat energy. Geothermal resources in some of the hot and warm basins in eastern and central China are fed by elevated heat flux from mantle upwelling in a rifting tectonic background. Typical examples are the Bohaibay,Songliao and Guanzhong Basins. Mantle heat also dominats those geothermal systems found in regions with recent volcanic activities. Good examples are Tengchong geothermal field in SW China and probably Changbaishan geothermal play in NE China. Deep faulting also induces heat accumulation in the crust that serves as an additional heat source, which is found in major fault zones. Tanlu fault zone in eastern China is a typical example of this kind, so are those in SE China, including Zhangzhou, Fuzhou geothermal fields. Geothermal systems with dominant radiogenic heat generation from radio nuclides have not been confirmed but maybe a new type to be discovered in China. This classification scheme is expected to be more effective in guiding exploration and assessment of geothermal resources. The search for deep geothermal resources (hot dry rock) should also benefit from this fundamental understanding and theoretical classification

Deep groundwater cycle in Xiongxian geothermal field

ABSTRACT The deep karstic aquifer containing hot water is ideal for space heating and maybe also for geothermal power generation. The hot water is characterized by high single-well yield, low salinity, gravity injection and less impact on environment when it is exploited. In order to run a karstic geothermal field sustainably, it is of high importance to identify the local groundwater circulation pattern in the field. Here we take Xiongxian geothermal field as an example to get insights into the characteristics of local groundwater circulation in karstic aquifers. Groundwater samples and surface water samples were collected, and analyzed for their hydrochemical and isotopic contents. Results show that the TDS of groundwater in karstic aquifers is between 1.9 and 2.6 g/L. According to the data of water table, the local groundwater flow direction is from Southwest to Northeast. This is confirmed by the TDS data, which increases gradually in this direction. However, this is orthogonal to the regional groundwater flow direction that is from Northwest to Southeast. This phenomenon highlights the control of aquifer lithology and geological structures on the groundwater flow field. Isotopic data illustrates some oxygen isotope shifts, although the reservoir temperature is less than 90 ℃. Finally, a conceptual model is proposed to depict the deep groundwater cycle in Xiongxian geothermal field, which will serve as a basis for the further simulated model for exploitation strategy and could be used as a reference in similar karstic aquifers

Prevalence and Characterization of Food-Related Methicillin-Resistant Staphylococcus aureus (MRSA) in China

Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging pathogen that is difficult to treat due to the multiresistance of the bacteria upon infection. From 2011 to 2016, 1581 S. aureus strains were isolated from 4300 samples from retail foods covering most provincial capitals in China. To determine the prevalence of food-related MRSA and its genetic background in China, antibiotic resistance, staphylococcal toxin genes, staphylococcal cassette chromosome mec (SCCmec) typing, spa-typing and MLST were carried out in this study. In total, 108 (7.4%) isolates were confirmed for MRSA by phenotyping (cefoxitin) and genotyping (mecA/mecC gene). A total of 52.8% (57/108) of the MRSA isolates belonged to clonal complex 59 (CC59) (ST59, ST338, and ST3355), which was the predominant clone in this study. These CC59 isolates carried SCCmec elements of type IV, V, or III and exhibited spa type t437, t441, t543, t163, t1785, or t3485, and half of them carried major virulence genes, such as the Panton-Valentine leucocidin (PVL) gene. The secondary clones belonged to ST9 (15.7%, 17/108) with a type of t899-SCCmec III and showed a broader range of antimicrobial resistance. The remaining MRSA isolates (31.5%, 34/108) were distributed in 12 different STs and 18 different spa types. All isolates harbored at least one of the enterotoxin genes, whereas only 4 isolates (3.70%) were positive for the toxic shock syndrome toxin tsst alleles. For antibiotic susceptibility testing, all isolates were resistant to more than three antibiotics, and 79.6% of the isolates were resistant to more than 10 antibiotics. Amoxycillin/clavulanic acid, ampicillin, cefoxitin, penicillin, ceftazidime, kanamycin, streptomycin, clindamycin, and telithromycin was the most common antibiotic resistance profile (55.6%, 60/108) in the study. In summary, the results of this study implied that the major food-related MRSA isolate in China was closer to community-associated MRSA, and some of the remaining isolates (ST9-t899-SCCmec III) were supposed to livestock-associated MRSA. In addition, most MRSA isolates showed resistance to multiple drugs and harbored staphylococcal toxin genes. Thus, the pathogenic potential of these isolates cannot be ignored. In addition, further studies are needed to elucidate the transmission routes of MRSA in relation to retail foods and to determine how to prevent the spread of MRSA

Isolation, Potential Virulence, and Population Diversity of Listeria monocytogenes From Meat and Meat Products in China

Listeria monocytogenes is a globally notorious foodborne pathogen. This study aimed to qualitatively and quantitatively detect L. monocytogenes from meat and meat products in China and to establish their virulence profiles and population diversity. From 1212 meat and meat product samples, 362 (29.9%) were positive for L. monocytogenes. Of these positive samples, 90.6% (328/362) had less than 10 MPN/g, 5.5% (20/364) samples had 10–110 MPN/g, and 3.9% (14/362) of the positive samples had over 110 MPN/g. Serogroup analysis showed that the most prevalent serogroup of L. monocytogenes was I.1 (1/2a-3a), which accounted for 45.0% (123/458) of the total, followed by serogroup I.2 (1/2c-3c) that comprised 26.9%, serogroup II.1 (4b-4d-4e) that comprised 4.8%, and serogroup II.2 (1/2b-3b-7) that comprised 23.3%. A total of 458 isolates were grouped into 35 sequence types (STs) that belonged to 25 clonal complexes (CCs) and one singleton (ST619) by multi-locus sequence typing. The most prevalent ST was ST9 (26.9%), followed by ST8 (17.9%), ST87 (15.3%), ST155 (9.4%), and ST121 (7.6%). Thirty-seven isolates harbored the llsX gene (representing LIPI-3), and they belonged to ST1/CC1, ST3/CC3, ST288/CC288, ST323/CC288, ST330/CC288, ST515/CC1, and ST619, among which ST323/CC288, ST330/CC288, and ST515/CC1 were newly reported to carry LIPI-3. Seventy-five isolates carried ptsA, and they belonged to ST87/CC87, ST88/CC88, and ST619, indicating that consumers may be exposed to potential hypervirulent L. monocytogenes. Antibiotics susceptibility tests revealed that over 90% of the isolates were susceptible to 11 antibiotics; however, 40.0% of the isolates exhibited resistance against ampicillin and 11.8% against tetracycline; further, 45.0 and 4.6% were intermediate resistant and resistant to ciprofloxacin, respectively. The rise of antibiotic resistance in L. monocytogenes suggests that stricter regulations should be formulated to restrict the use of antibiotic agents in human listeriosis treatment and livestock breeding

Bacillus cereus Isolated From Vegetables in China: Incidence, Genetic Diversity, Virulence Genes, and Antimicrobial Resistance

Bacillus cereus is a food-borne opportunistic pathogen that can induce diarrheal and emetic symptoms. It is widely distributed in different environments and can be found in various foods, including fresh vegetables. As their popularity grows worldwide, the risk of bacterial contamination in fresh vegetables should be fully evaluated, particularly in vegetables that are consumed raw or processed minimally, which are not commonly sterilized by enough heat treatment. Thereby, it is necessary to perform potential risk evaluation of B. cereus in vegetables. In this study, 294 B. cereus strains were isolated from vegetables in different cities in China to analyze incidence, genetic polymorphism, presence of virulence genes, and antimicrobial resistance. B. cereus was detected in 50% of all the samples, and 21/211 (9.95%) of all the samples had contamination levels of more than 1,100 MPN/g. Virulence gene detection revealed that 95 and 82% of the isolates harbored nheABC and hblACD gene clusters, respectively. Additionally, 87% of the isolates harbored cytK gene, and 3% of the isolates possessed cesB. Most strains were resistant to rifampicin and β-lactam antimicrobials but were sensitive to imipenem, gentamicin, ciprofloxacin, kanamycin, telithromycin, ciprofloxacin, and chloramphenicol. In addition, more than 95.6% of the isolates displayed resistance to three kinds of antibiotics. Based on multilocus sequence typing, all strains were classified into 210 different sequence types (STs), of which 145 isolates were assigned to 137 new STs. The most prevalent ST was ST770, but it included only eight isolates. Taken together, our research provides the first reference for the incidence and characteristics of B. cereus in vegetables collected throughout China, indicating a potential hazard of B. cereus when consuming vegetables without proper handling

Fault-Affected Fluid Circulation Revealed by Hydrochemistry and Isotopes in a Large-Scale Utilized Geothermal Reservoir

A new significant aspect in the utilization of hydrothermal energy in China is the large-scale exploitation using multiwells from a single geothermal site. This requires detailed hydrogeochemical investigations to gain insight about deep groundwater circulation. At the Xiongxian karst geothermal site in North China, where the demonstration project of large-scale utilization was conducted, 40 boreholes with depths from 1000 to 1800 m were drilled in a region of 50 km2. A total of 25 water samples were collected, and temperature loggings were conducted in 16 of these wells. At the site scale, the hydraulic head was observed to decline from SW to NE, i.e., orthogonal to that at the regional scale. Moreover, the geothermal groundwater temperature, borehole temperature gradient, and heat flow in the caprock all exhibited the same spatial trend with the groundwater head. Based on the hydrogeochemical and temperature logging data, this was explained by mixing of lateral recharging groundwater with ascending thermal fluids through the Xiongxian Fault, after excluding the causes of pumping activities and geologic structure. In addition, geothermal groundwater 81Kr age was estimated to be approximately 760 k yr, which is much older than the 14C age of 20 to 30 k yr. The older 81Kr age implies a low renewability of deep groundwater circulation, which should be considered in terms of sustainable management in relation to the large-scale utilization of geothermal resources

An exploration on investigation and mapping of ground substrate in Ruyi River Basin, Bashang Plateau, Chengde City

The investigation and mapping of ground substrate is a whole new field of natural resources survey and monitoring system in the current situation, which has not been evolved into a unified guideline on action. In this paper, based on the summarization of the current situation of the Earth’s critical zone theory and ground substrate survey, fully drawing on the investigation methods and map expression styles of regional geology, engineering geology, Quaternary geology, hydrogeology, environmental geology, etc., and taking Ruyi River Basin in Bashang Plateau of Chengde City as the research area, we establish the method of the investigation and mapping of ground substrate based on the theory of the Earth’s critical zone. Based on the field investigation results, the ground substrate in Ruyi River Basin can be divided into nine types as follows: basalt, andesite, tuff, rhyolite, alluvial-proluvial gravel, eluvial-proluvial gravel, aeolian sand, lacustrine silt and marsh silt. The distribution characteristics as well as the investigation and monitoring indicators in different types of ground substrates are also studied. The spatial distribution map of aeolian sand thickness, coupling relationship profile between the ground substrate and the ground covered layer, and ground substrate map are drew. The spatial distribution map of ground substrate thickness can show the spatial distribution characteristics of the thickness of different types of surface substrate layers. The coupling relationship profile of ground substrate and surface covered layer can directly describe the constraint of the profile structural characteristics and physical and chemical properties of ground substrate on the ecological elements of the covered layer. The ground substrate map can systematically reflect the structure, distribution, characteristics and coupling relationship between ground substrate and ground covered layer. The series maps of ground substrate are the reflection of the interaction of multiple cycles in the Earth’s critical zone, such as the rock, the soil, the water, and the organism. The results of this study can provide references for the nationwide investigation and mapping of the ground substrate

The Genesis Mechanism and Health Risk Assessment of High Boron Water in the Zhaxikang Geothermal Area, South Tibet

The natural discharge of geothermal water containing harmful components affects the water quality of the surrounding environment and brings security risks to drinking water safety. The geothermal water in Tibet is characterized by high boron content, but the water pollution caused by the discharge of this high boron geothermal water is not clear. In this study, we collected geothermal water and surface water from the Zhaxikang geothermal system in southern Tibet to investigate the causes of high boron geothermal water and the water pollution of water quality by its discharge. The results indicate that the hydrochemical type of geothermal water was HCO3-Cl-Na, while that of cold spring water, mine water, river water, and lake water was SO4-HCO3-Ca-Mg. Hydrogen and oxygen isotopes show that the recharge source of cold groundwater was mainly snow-melting water and meteoric water, while in addition to that, there is magmatic water for hot springs. The boron content of geothermal water in the study area is as high as 42.36 mg/L, far exceeding the World Health Organization limit for drinking water (0.5 mg/L). The analysis of ion components and PHREEQC modeling indicated that the dissolution of silicate minerals and cation exchange controlled the composition of groundwater, and the boron in groundwater mainly came from the volatilization of magmatic components and the leaching of shallow sediments. The entropy weight water quality index was used to evaluate the water quality of the study area; about 42.9% of the groundwater samples are of good quality and can be used for drinking, mainly cold water that has not been mixed with geothermal water in the upstream. With the discharge of geothermal water into the river (with a mix ratio of ~20%), the downstream water quality gradually deteriorated. The health risk assessment of drinking water in the study area showed that the hazard index (HI) of drinking water in the mixed area was higher than 1 (with an average of 1.594 for children and 1.366 for adults), indicating that children are at a higher health risk than adults. Geothermal water with high boron content has been found all over the world, and the adverse effects of its natural drainage cannot be ignored

Differentiation of Bacillus cereus and Bacillus thuringiensis Using Genome-Guided MALDI-TOF MS Based on Variations in Ribosomal Proteins

Bacillus cereus and B. thuringiensis are closely related species that are relevant to foodborne diseases and biopesticides, respectively. Unambiguous differentiation of these two species is crucial for bacterial taxonomy. As genome analysis offers an objective but time-consuming classification of B. cereus and B. thuringiensis, in the present study, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to accelerate this process. By combining in silico genome analysis and MALDI-TOF MS measurements, four species-specific peaks of B. cereus and B. thuringiensis were screened and identified. The species-specific peaks of B. cereus were m/z 3211, 6427, 9188, and 9214, and the species-specific peaks of B. thuringiensis were m/z 3218, 6441, 9160, and 9229. All the above peaks represent ribosomal proteins, which are conserved and consistent with the phylogenetic relationship between B. cereus and B. thuringiensis. The specificity of the peaks was robustly verified using common foodborne pathogens. Thus, we concluded that genome-guided MALDI-TOF MS allows high-throughput differentiation of B. cereus and B. thuringiensis and provides a framework for differentiating other closely related species