Wild mice were sampled from across the southern UK.

<p>The 12 sampling sites are shown by 2-letter designations, with the number of animals obtained at each site shown in parentheses.</p

Mice vary in their immunological distance.

<p>The immunological distance among mice (i) within each sample site is shown as the size of the coloured circles, and the immune distance among sites is shown as the 2-dimensional immunological distance following multidimensional scaling (MDS). Within sample site and among sample sites are therefore shown on different scales. Sample sites are colour coded, and 2-letter designations are as shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.g001" target="_blank">Fig 1</a>.</p

Mice have strongly genetically structured populations.

<p>A neighbour-joining tree showing the relationship among mice. The site colour coding and 2-letter site designations are as <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.g001" target="_blank">Fig 1</a>. The scale is the number of nucleotide differences among individuals. Mice numbers 1–12 are control laboratory mice (LAB), where 1 and 2 are L88 and L90 C57BL/6 mice as in [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.ref001" target="_blank">1</a>], 3 is C57/BL6J, 4 is SJL/J, 5 is FVB/NJ, 6 is NOD/LJ, 7 is BALB/cJ, 8 is AKR/J, 9 is DBA/J, 10 is C3H/HeJ, 11 is CBA/J, and 12 is 129S1/SvlmJ and where 3–12, inclusive, are data obtained from <a href="http://support.illumina.com/array/array_kits/mouse_md_linkage/downloads.html" target="_blank">http://support.illumina.com/array/array_kits/mouse_md_linkage/downloads.html</a>.</p

Immunological distance is not explained by genetic or physical distance.

<p>Tanglegrams based on unweighted pair group method with arithmetic mean (UPGMA) trees of immunological distance, genetic distance, as F_st, and geographical distance among sites, where the scales are the relevant measures (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.s013" target="_blank">S1</a>, <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.s014" target="_blank">S2</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.s015" target="_blank">S3</a> Tables) of (A) immunological and genetic, (B) immunological and geographical, and (C) genetic and geographical distance among mice from the different sites. The site colour coding is as in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003538#pbio.2003538.g001" target="_blank">Fig 1</a>.</p

Mycobacteria-specific MBC responses in BCG vaccinated and unvaccinated donors.

<p>Frequencies of antigen-specific MBCs were determined by ELISPOT and are presented as a percentage of the total IgG secreting cells. (<b>A</b>) and (<b>B</b>) Frequencies of mycobacteria-specific MBCs in BCG vaccinated (closed circles) and unvaccinated (open circles) donors, (<b>C</b>) MBC responses in vaccinated donors with (closed circles) or without (open circles) any travel history to <i>M. tuberculosis</i> endemic areas. (<b>D</b>) PPD-specific MBC responses in BCG vaccinated individuals without detectable ESAT-6 or CFP-10 MBC responses. ***, P<0.001; **, P<0.01.</p

Mycobacteria-specific antibody responses in BCG vaccinated and unvaccinated donors.

<p>Antibody responses were determined by ELISA. (<b>A</b>) PPD, TT and DT specific antibody levels in BCG vaccinated (closed circles) and unvaccinated donors (open circles). (<b>B</b>) Mycobacteria (Ag85A, Ag85B, ESAT-6, CFP-10 and PPD) specific antibody levels in BCG vaccinated (closed circles) and unvaccinated donors (open circles). (<b>C</b>) Antibody levels in vaccinated donors with (closed circles) or without (open circles) any travel history to <i>M. tuberculosis</i> endemic areas. (<b>D</b>) PPD-specific antibody levels in BCG vaccinated individuals without detectable ESAT-6 or CFP-10 antibody responses.</p

Mycobacteria-specific IFN-γ responses in BCG vaccinated and unvaccinated donors.

<p>Responses were measured by stimulating whole blood for 6 days and assaying the culture supernatants for IFN-γ by ELISA. (<b>A</b>) Mycobacteria-specific IFN-γ production in BCG vaccinated (closed circles) unvaccinated (open circles) donors. (<b>B</b>) IFN-γ responses in BCG vaccinated donors with (closed circles) or without (open circles) any travel history to <i>M. tuberculosis</i> endemic areas. (<b>C</b>) PPD-specific IFN-γ in BCG vaccinated donors lacking ESAT-6 or CFP-10 responses. *, P<0.05, ***, P<0.001.</p