Alignment of five Hsp70 isoforms from <i>E</i>. <i>superba</i> and <i>E</i>. <i>crystallorophias</i>.

<p>In red: Hsp70 diagnostic motifs; in black: possible glycosylation sites; in blue: hydrophobic linker between Nucleotide Binding Domain and Substrate Binding Domain.</p

Curves representing the loss of mobility of a population of krill subjected to gradual temperature increase (0.1.min^-1) (<i>E</i>. <i>crystallorophias</i> n = 130 blue curve; <i>E</i>. <i>superba</i> n = 43 red curve).

<p>CT_max is the temperature at which 50% of mobile animals are reached.</p

Expression levels of <i>hsp70</i> genes obtained by qPCR in two krill species: <i>E</i>. <i>superba</i> (red solid line) and <i>E</i>. <i>crystallorophias</i> (blue dotted line) during a heat shock of 3°C and 6°C whereas 0h group is control at 0°C.

<p>Heat shocks were carried out during 3h and 6h, and hsp70 expressions measured at those time on n = 6 to 10 individuals per group. Hsp70 expressions for both species were normalized by the <i>18S</i> gene expression. To compare values, a Kruskal-Wallis test followed by a Dunn’s test were used to compare control groups (0h) and shocked groups (3h and 6h). Significant differences between mean normalized expressions are indicated by asterisks (*).</p

Phylogeny of the HSP70 family in Eucrustacea based on Maximum Likelihood and Bayesian analyses of amino acid data set.

<p>Numbers above branches are posterior probabilities and bootstrap values (based on 100 replicated).</p

Number of synonymous and non-synonymous mutations between the two krill species.

<p>N.dN and S.dS estimates obtained between <i>E</i>. <i>superba</i> and <i>E</i>. <i>crystallorophias</i> for the 5 paralogous <i>Hsp70</i> genes using the Nei-Gojobori method [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121642#pone.0121642.ref079" target="_blank">79</a>] to make evolutionary-rate comparisons under the assumption of a global molecular clock.</p><p>Number of synonymous and non-synonymous mutations between the two krill species.</p

Non-synonymous (dN) tree of the 11 Hsp70 genes found in <i>E</i>. <i>superba</i> and <i>E</i>. <i>crystallorophias</i> obtained using the free-ratio (M1) branch model of CodeML according to the Goldman & Yang (1994) model of codon substitution.

<p>Above internal branches: dN/dS (ω) ratio, below internal branches: number of non-synonymous and synonymous mutations estimated from the ancestral sequence reconstruction. * indicates saturation at the synonymous sites. The green colour represents branches under putative selective relaxation and the red one, branches (mostly terminal) under positive selection. Next to external (terminal) branches also corresponds to the ω ratio and the number of non-synonymous and synonymous mutations obtained from the ancestral sequence reconstruction using the M_2A branch-site model of selection. If any, a list of amino-acid replacements that corresponds to positively selected sites with a BEB probability greater than 90% is provided together with these values.</p

<i>E</i>. <i>crystallorophias Hsp70</i> isoform expression values and associated Blast matches.

<p>comp ID: sequences assembled with Trinity. Size (aa): deduced coding sequences. Size (pb): comp or contig sizes in pair bases. FPKM = Fragments Per Kilobase of exon per Million fragments mapped. Values of the isoform TPM or FPKM are in bold.</p><p><i>E</i>. <i>crystallorophias Hsp70</i> isoform expression values and associated Blast matches.</p