Effect of prolactin on the phosphorylation of Ca-dependent PKC isoforms

<p><b>Copyright information:</b></p><p>Taken from "Prolactin stimulates the proliferation of normal female cholangiocytes by differential regulation of Ca-dependent PKC isoforms"</p><p>http://www.biomedcentral.com/1472-6793/7/6</p><p>BMC Physiology 2007;7():6-6.</p><p>Published online 19 Jul 2007</p><p>PMCID:PMC1939715.</p><p></p> Immunoblots for PKC-α, PKC-β-I, PKC-β-II and PKC-γ in normal female cholangiocytes stimulated for 90 minutes at 37°C with 0.2% BSA (basal value) or prolactin (100 nM) with 0.2% BSA. When cholangiocytes were treated with prolactin, there was an increase in the phosphorylation of PKCβ-I and a marked decrease in PKCα phosphorylation; no significant changes in the phosphorylation of PKCβ-II and PKCγ were observed in normal female cholangiocytes treated with prolactin or 0.2% BSA. Data are mean ± SEM of 3 experiments. * p < 0.05 vs. corresponding basal values. PKC = protein kinase C

Measurement of the number of top panel PCNA- and lower panel CK-19-positive cholangiocytes in liver sections (5 μm, 3 slides analyzed per group) and c PCNA protein expression in purified female cholangiocytes from NaCl- or prolactin-treated rats

<p><b>Copyright information:</b></p><p>Taken from "Prolactin stimulates the proliferation of normal female cholangiocytes by differential regulation of Ca-dependent PKC isoforms"</p><p>http://www.biomedcentral.com/1472-6793/7/6</p><p>BMC Physiology 2007;7():6-6.</p><p>Published online 19 Jul 2007</p><p>PMCID:PMC1939715.</p><p></p> Administration of prolactin to normal female rats increased the number of PCNA-positive cholangiocytes (arrows) and CK-19-positive cholangiocytes compared with normal rats treated with NaCl. Orig. magn., ×20 (PCNA) and ×10 (CK-19). Data are mean ± SEM of 5 values obtained from the 3 slides evaluated per each group of animal. * p < 0.05 vs. the corresponding value of NaCl-treated rats