Estimated effects (E) and Pillai's trace p-values (p) for a two-way MANOVA scheme comprising simple and interaction effects of PLV and FIVC, for each day of analysis.

a<p>Significant results are in bold.</p>b<p>This value is not significant if the MANOVA is run without imputing the values (p = 0.06).</p

Pearson's coefficients of the ten strongest (absolute) pair-wise correlations between immunological responses for each day of analysis.

a<p>Pair-wise correlations that increase (bold) or decrease (italics) are highlighted.</p>b<p>The sign indicates whether a pair co-varies positively or negatively.</p>c<p>All coefficients are statistically significant (p-values substantially smaller than 0.05) using a test for association/correlation between paired sample, except for the two negative correlations of Lymph with TNFα and IL10 at day 52, (TNFα p-value  = 0.13, IL10 p-value  = 0.12).</p

Total variances within infection status groups on different days.

a<p>The total variance for all cats (last row) is 11 on each day because of normalization.</p

(A) Box-plots of (absolute) pair-wise correlations and (B) Scree plots from PCA.

<p>(A) The horizontal axis indicates the four days of analysis, and the vertical axis shows the distribution of absolute pair-wise correlations among the 11 immunological response parameters. Open circles indicate parameter pairs with particularly strong correlations (“outliers” in the box-plots). Note that on day 52, the majority of parameters have lower correlations overall, but there are several that are highly correlated (anti-correlated). (B) The ordered PCA components are shown on the horizontal axis (EV stands for eigenvector). The vertical axis shows the share of overall variability explained by each component, in different colors for each of the four days of analysis.</p

Bi-plots from LDA, for days 31 (upper left), 37 (upper right), 52 (lower left) and 59 (lower right).

<p>The horizontal and vertical axes on each panel represent first and second LDA direction, respectively. The arrows depict loadings of the 11 responses; their orientation and length indicate the role of each immunological parameter in relation to the LDA directions and the different infection groups, on each of the analysis days. The insets in each panel show first, second and third LDA eigenvalue (discriminatory importance of the corresponding LDA directions).</p

List of immunological response parameters used in the analysis.

<p>List of immunological response parameters used in the analysis.</p