71 research outputs found
Spectral tuning of plasmon-enhanced silicon quantum dot luminescence
In the presence of nanoscale silver island arrays, silicon quantum dots exhibit up to sevenfold luminescence enhancements at emission frequencies that correspond to the collective dipole plasmon resonance frequency of the Ag island array. Using electron-beam lithography to alter the pitch and particle diameter, this wavelength-selective enhancement can be varied as the metal array resonance wavelength is tuned from 600 to 900 nm. The luminescence intensity enhancement upon coupling is attributed to an increase in the radiative decay rate of the silicon quantum dots
Phosphine Functionalization of GaAs(111)A Surfaces
Phosphorus-functionalized GaAs surfaces have been prepared by exposure of Cl-terminated GaAs(111)A surfaces to triethylphosphine (PEt3) or trichlorophosphine (PCl3), or by the direct functionalization of the native-oxide terminated GaAs(111)A surface with PCl3. The presence of phosphorus on each functionalized surface was confirmed by X-ray photoelectron spectroscopy. High-resolution, soft X-ray photoelectron spectroscopy was used to evaluate the As and Ga 3d regions of such surfaces. On PEt3 treated surfaces, the Ga 3d spectra exhibited a bulk Ga peak as well as peaks that were shifted to 0.35, 0.92 and 1.86 eV higher binding energy. These peaks were assigned to residual Cl-terminated Ga surface sites, surficial Ga2O and surficial Ga2O3, respectively. For PCl3-treated surfaces, the Ga 3d spectra displayed peaks ascribable to bulk Ga(As), Ga2O, and Ga2O3, as well as a peak shifted 0.30 eV to higher binding energy relative to the bulk signal. A peak corresponding to Ga(OH)3, observed on the Cl-terminated surface, was absent from all of the phosphine-functionalized surfaces. After reaction of the Cl-terminated GaAs(111)A surface with PCl3 or PEt3, the As 3d spectral region was free of As oxides and As0. Although native oxide-terminated GaAs surfaces were free of As oxides after reaction with PCl3, such surfaces contained detectable amounts of As0. Photoluminescence measurements indicted that phosphine-functionalized surfaces prepared from Cl-terminated GaAs(111)A surfaces had better electrical properties than the native-oxide capped GaAs(111)A surface, while the native-oxide covered surface treated with PCl3 showed no enhancement in PL intensity
Single-Molecule Super-Resolution Imaging of TcpP Dynamics in Vibrio Cholerae in Response to Virulence Pathway Deactivation by Increased Cell Density
Enhanced Radiative Emission Rate and Quantum Efficiency in Coupled Silicon Nanocrystal-Nanostructured Gold Emitters
We report local-field-enhanced light emission from silicon nanocrystals close to a film of nanoporous gold. We resolve photoluminescence as the goldāSi nanocrystal separation distance is varied between 0 and 20 nm and observe a fourfold luminescence intensity enhancement concomitant with increases in the coupled silicon nanocrystal/nanoporous gold absorbance cross section and radiative decay rate. A detailed analysis of the luminescence data indicated a local-field-enhanced quantum efficiency of 58% for the Si nanocrystals coupled to the nanoporous gold layer
Singleāmolecule tracking in live Vibrio cholerae reveals that ToxR recruits the membraneābound virulence regulator TcpP to the toxT promoter
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110815/1/mmi12834.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/110815/2/mmi12834-sup-0001-si.pd
Addressing the Requirements of HighāSensitivity SingleāMolecule Imaging of LowāCopyāNumber Proteins in Bacteria
Singleāmolecule fluorescence superāresolution imaging and tracking provide nanometerāscale information about subcellular protein positions and dynamics. These singleāmolecule imaging experiments can be very powerful, but they are best suited to highācopy number proteins where many measurements can be made sequentially in each cell. We describe artifacts associated with the challenge of imaging a protein expressed in only a few copies per cell. We image live Bacillus subtilis in a fluorescence microscope, and demonstrate that under standard singleāmolecule imaging conditions, unlabeled B. subtilis cells display punctate red fluorescent spots indistinguishable from the few PAmCherry fluorescent protein single molecules under investigation. All Bacillus species investigated were strongly affected by this artifact, whereas we did not find a significant number of these background sources in two other species we investigated, Enterococcus faecalis and Escherichia coli. With singleāmolecule resolution, we characterize the number, spatial distribution, and intensities of these impurity spots.Bright spots: A singleāmoleculeālike fluorescent background signal is reported in Bacillus subtilis cells, and the density and fluorescence intensity of these spots are quantified in several Bacillus species and other Gramānegative and Gramāpositive organisms.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144710/1/cphc201600035_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144710/2/cphc201600035.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144710/3/cphc201600035-sup-0001-misc_information.pd
Chip-scale photonics with plasmonic components
In this talk we will describe recent opportunities presented by plasmonics for chip-scale integration of photonic and electronic devices, including i)design of metal-insulator-metal plasmon waveguides that optimize the trade-off between mode localization and propagation loss in the visible and near-infrared ii) on-chip Si CMOS compatible light near-infrared light sources for coupling into plasmonic networks iii) plasmon-enhanced emission from quantum dots, and iv) opportunities for active plasmonic devices based on electro-optic and all-optical modulation of plasmon propagation
Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes
Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies
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