rPA antibody-specific Toxin Neutralization Assay (TNA) and ED50 values (μg/mL).

<p>(A) generated from vaccinated wild type and (B) mutant rPA (geometric mean with error bars representing the 95% confidence interval).</p

Kaplan-Meier Survival Curve of vaccinated, unvaccinated control, and sham vaccinated control rabbits.

<p>Kaplan-Meier Survival Curve of vaccinated, unvaccinated control, and sham vaccinated control rabbits.</p

Recovery of <i>B</i>. <i>anthracis</i> from Select Tissues of vaccinated, unvaccinated control, and sham vaccinated control rabbits.

<p>Recovery of <i>B</i>. <i>anthracis</i> from Select Tissues of vaccinated, unvaccinated control, and sham vaccinated control rabbits.</p

Experimental Procedures and Schedule.

<p>^aThrice daily observations performed on Days 72–74. Twice daily observations performed other days.</p><p>^bBody weight obtained at randomization, on Day 0 and weekly thereafter.</p><p>^cAnimals vaccinated via intramuscular injection on Day 0 or Days 0 and 28 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130952#pone.0130952.t001" target="_blank">Table 1</a>).</p><p>^dBlood collected and sera isolated for TNA and ELISA on Days -6, 14, 28, 42 and 65 ± 4, when moribund euthanized or at terminal euthanasia (Day 84).</p><p>^eRabbits challenged with 200 x ± 50 LD₅₀<i>B</i>. <i>anthracis</i> (Ames) spores. The published inhalation LD₅₀ for NZW rabbits is 1.1 x 10⁵ spores.</p><p>^fBlood collected for CBC and differential.</p><p>^gBlood collected and sera isolated for clinical chemistry parameters.</p><p>^hBlood collected for quantitative bacteriology.</p><p>ⁱBlood collected and sera isolated for electrochemiluminescence assay (ECL).</p><p>^jMoribund euthanized or found dead rabbits received a limited gross necropsy.</p><p>^kEuthanized or found dead rabbits received a gross necropsy and select tissues collected for bacteriology or histopathology.</p><p>Experimental Procedures and Schedule.</p

Study Summary of Fate and Immune Status Relevant to B. anthracis Challenge on Day 70.

<p>^aLower limit of quantitation < 30 μg/mL.</p><p>^bSamples which returned an incalculable ED50 (by Gen 5 software) were assigned an ED50 value of 1.</p><p>Study Summary of Fate and Immune Status Relevant to B. anthracis Challenge on Day 70.</p

p Values Associated with Comparison of Ig anti-PA Values between Vaccine Groups as Analyzed by Repeated Measures ANOVA.

<p>Ig anti-PA levels from blood draws between day -1 and 65 were analyzed by repeated measures ANOVA.</p><p>The Ig anti-PA response of non-vaccinated groups (None and Vehicle, not shown above) were significantly lower (p < 0.0001) than every other group except each other.</p><p>^↓Indicates the Ig anti-PA response of the vaccine group listed on the left is significantly less than the corresponding vaccine group listed on the top of the table.</p><p>^↑Indicates the Ig anti-PA response of the vaccine group listed on the left is significantly greater than the corresponding vaccine group listed on the top of the table.</p><p>NS, no statistical significance between the two groups.</p><p>p Values Associated with Comparison of Ig anti-PA Values between Vaccine Groups as Analyzed by Repeated Measures ANOVA.</p