Organismo geneticamente modificado com proteínas derivadas de espécies pertencentes ao gênero crassostrea, processo de biorremediação de um ambiente compreendendo metais pesados, e processo de biomineração de um ambiente compreendendo metais pesados

genetically modified organism with proteins derived from species belonging to the genus crassostrea, process of bioremediation of an environment comprising heavy metals, and process of biomineration of an environment comprising heavy metals the present invention describes genetically modified organisms capable of expressing a synthetic gene with multiple copies of metal binding domains of crassostrea rhizophorae metallothionein. this new organism is capable of removing heavy metals from environments such as soils, natural and industrial fluids. the present invention also describes a product for bioremediation that contains this organism and a process for bioremediation of environments comprising heavy metals. the present invention further describes a product for the biomineration that contains this organism and for a heavy metal biormination process

A hybrid-hierarchical genome assembly strategy to sequence the invasive golden mussel Limnoperna fortunei

ABSTRACT Background: For more than 25 years, the golden mussel Limnoperna fortunei has aggressively invaded South American freshwaters, having travelled more than 5,000 km upstream across five countries. Along the way, the golden mussel has outcompeted native species and economically harmed aquaculture, hydroelectric powers, and ship transit. We have sequenced the complete genome of the golden mussel to understand the molecular basis of its invasiveness and search for ways to control it. Findings: We assembled the 1.6 Gb genome into 20548 scaffolds with an N50 length of 312 Kb using a hybrid and hierarchical assembly strategy from short and long DNA reads and transcriptomes. A total of 60717 coding genes were inferred from a customized transcriptome-trained AUGUSTUS run. We also compared predicted protein sets with those of complete molluscan genomes, revealing an exacerbation of proteinbinding domains in L. fortunei. Conclusions: We built one of the best bivalve genome assemblies available using a cost-effective approach using Illumina pair-end, mate pair, and PacBio long reads. We expect that the continuous and careful annotation of L. fortunei's genome will contribute to the investigation of bivalve genetics, evolution, and invasiveness, as well as to the development of biotechnological tools for aquatic pest control

Gene Discovery through Transcriptome Sequencing for the Invasive Mussel <i>Limnoperna fortunei</i>

<div><p>The success of the Asian bivalve <i>Limnoperna fortunei</i> as an invader in South America is related to its high acclimation capability. It can inhabit waters with a wide range of temperatures and salinity and handle long-term periods of air exposure. We describe the transcriptome of <i>L. fortunei</i> aiming to give a first insight into the phenotypic plasticity that allows non-native taxa to become established and widespread. We sequenced 95,219 reads from five main tissues of the mussel <i>L. fortunei</i> using Roche’s 454 and assembled them to form a set of 84,063 unigenes (contigs and singletons) representing partial or complete gene sequences. We annotated 24,816 unigenes using a BLAST sequence similarity search against a NCBI nr database. Unigenes were divided into 20 eggNOG functional categories and 292 KEGG metabolic pathways. From the total unigenes, 1,351 represented putative full-length genes of which 73.2% were functionally annotated. We described the first partial and complete gene sequences in order to start understanding bivalve invasiveness. An expansion of the hsp70 gene family, seen also in other bivalves, is present in <i>L. fortunei</i> and could be involved in its adaptation to extreme environments, e.g. during intertidal periods. The presence of toll-like receptors gives a first insight into an immune system that could be more complex than previously assumed and may be involved in the prevention of disease and extinction when population densities are high. Finally, the apparent lack of special adaptations to extremely low O₂ levels is a target worth pursuing for the development of a molecular control approach.</p></div

Quantity of contigs by number of reads assembled.

<p>Most contigs (2,687, representing 58% of total) resulted from the assembly of two single reads. Only nine contigs were formed by 40 or more reads. Triangles represent <i>L. fortunei</i> contigs.</p

Functional annotation of the <i>L. fortunei</i> transcriptome.

<p>Functional annotation of the <i>L. fortunei</i> transcriptome.</p

Number of annotated transcripts related to cellular defense.

<p>Number of annotated transcripts related to cellular defense.</p

Summary of the cleaning procedure for <i>L. fortunei</i> transcriptomic data.

a<p>Crossmatch data, after cleaning.</p